A5100610884- Pulmonary Hypertension Research and Treatments
- Extracellular vesicles in disease
- Cardiovascular Function and Risk Factors
- RNA modifications and cancer
- Nerve injury and regeneration
- Dialysis and Renal Disease Management
- Congenital heart defects research
- Cancer, Hypoxia, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA and protein synthesis mechanisms
- Protein Interaction Studies and Fluorescence Analysis
- RNA Research and Splicing
- Temporomandibular Joint Disorders
- ATP Synthase and ATPases Research
- Bone Metabolism and Diseases
- Osteoarthritis Treatment and Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Peroxisome Proliferator-Activated Receptors
- Congenital limb and hand anomalies
- Machine Learning in Bioinformatics
- Kruppel-like factors research
- Genomics, phytochemicals, and oxidative stress
- Liver Disease Diagnosis and Treatment
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
2024-2025
Chinese People's Liberation Army
2024
Jiangxi Maternal and Child Health Hospital
2023-2024
Chinese PLA General Hospital
2023-2024
University of Arizona
2021-2024
University of Phoenix
2020-2024
Guangxi University of Chinese Medicine
2023-2024
Sichuan University
2023-2024
Zhongshan Hospital of Xiamen University
2023-2024
Xiamen University
2023-2024
<h3>Objectives</h3> In this study, we aim to determine the effect of metformin on osteoarthritis (OA) development and progression. <h3>Methods</h3> Destabilisation medial meniscus (DMM) surgery was performed in 10-week-old wild type AMP-activated protein kinase (AMPK)α1 knockout (KO) mice. Metformin (4 mg/day drinking water) given, commencing either 2 weeks before or after DMM surgery. Mice were sacrificed 6 12 OA phenotype analysed by micro-computerised tomography (μCT), histology...
Abstract Background Nerve guide conduits are a promising strategy for reconstructing peripheral nerve defects. Improving the survival rate of seed cells in is still challenge and microcarriers an excellent three-dimensional (3D) culture scaffold. Here, we investigate effect 3D on biological characteristics adipose mesenchymal stem (ADSCs) to evaluate efficacy chitosan filled with loaded ADSCs repairing Methods In vitro, prepared porous microspheres by modified emulsion cross-linking method...
The mechanisms underlying myricetin-induced cancer cell apoptosis remain to be elucidated. Certain previous studies have shown that myricetin induces through the mitochondrial pathway. Apoptosis, however, can also induced by other classical pathways, including endoplasmic reticulum (ER) stress and DNA double‑strand breaks (DSBs). aim of present study was assess whether these two apoptotic pathways are involved in myricetin‑induced death SKOV3 ovarian cells. results revealed treatment with...
Inflammation and oxidative stress are common features of patients with chronic kidney disease (CKD) many uremic solutes retained in these could be involved processes, among which protein-bound such as indoxyl sulfate (IS). White adipose tissue recently gained attention an important source inflammation stress. To examine the effect IS on adipocytes, 3T3-L1 cells were incubated to mimic conditions encountered patients. Incubation increased reactive oxygen species production generated mainly...
Abstract 15‐Lipoxygenase/15‐hydroxyeicosatetraenoic acid (15‐LO/15‐HETE) is known to modulate pulmonary vascular medial hypertrophy and intimal endothelial cells migration angiogenesis after hypoxia. However, it unclear whether 15‐HETE affects the adventitia of arterial wall. We performed immunohistochemistry, fibrosis, artery fibroblasts phenotype extracellular matrix (ECM) deposition determine role in hypoxia‐induced remodeling. Our studies showed that O 2 deprivation induced arteries with...
The Antarctic continent has extreme natural environment and fragile ecosystem. Mosses are one of the dominant floras in continent. However, their genomic features adaptation processes to environments remain poorly understood. Here, we assembled high-quality genome sequence moss ( Pohlia nutans ) with 698.20 Mb 22 chromosomes. We found that high proportion repeat sequences a recent whole-genome duplication (WGD) contribute large size P. when compared other bryophytes. harbors signatures...
Flowers of Carthamus tinctorius L. are traditionally used in China to treat cerebrovascular and cardiovascular diseases. Hydroxysafflor yellow A (HSYA), the main constituent flowers, is known for its multiple biological activities. In present study, HSYA was isolated from flowers by a macroporous resin adsorption chromatography method coupled with Waters high-throughput auto-purification system it's vasodilatation effects on pulmonary artery (PA) were explored an assay tension study rat...
Nitrative stress is a characteristic feature of the pathology human pulmonary arterial hypertension. However, role nitrative in pathogenesis obliterative vascular remodelling and severe hypertension remains largely unclear.Our recently identified novel mouse model (Egln1Tie2Cre, Egln1 encoding prolyl hydroxylase 2 (PHD2)) has right heart failure, making it an excellent to use this study examine remodelling.Nitrative was markedly elevated whereas endothelial caveolin-1 (Cav1) expression...
Cell therapy based on mesenchymal stem cells (MSCs) alleviate muscle atrophy caused by diabetes and aging; however, the impact of human umbilical cord following nerve injury underlying mechanisms remain unclear. In this study, we evaluated therapeutic efficacy MSCs (hucMSCs) hucMSC-derived exosomes (hucMSC-EXOs) for identified molecular mechanisms. Sciatic crush in rats induction myotubes L6 were used to determine ameliorating effect hucMSCs hucMSC-EXOs atrophy. Q-PCR Western blot analyses...
This trail was conducted to study the effect of L-proline on growth performance, and blood parameter in weaned lipopolysaccharide (LPS)-challenged pigs. Thirty six pigs (9.13±0.85 kg) were assigned randomly dietary treatments a 2×3 factorial arrangement 20-d assay. Factors intraperitoneal injection with saline or LPS, three supplement levels (0%, 0.5%, 1.0%). On d 10, samples collected at 3 h after LPS (100 μg LPS/kg body weight [BW]) injection. 20 trial, all orally administrated D-xylose...
Background: Tanshinone IIA inhibits the proliferation of pulmonary artery smooth muscle cells (PASMCs), but potential mechanisms its effects on PASMCs apoptosis remain unclear. Methods: Rat were subjected to hypoxia for 9 days with or without treatment. exposed conditions 2% O2 and 93% N2 24 h in vitro. Hematoxylin eosin (HE) staining was used evaluate vascular remodeling. The Cell viability determined using cell fluorescence MTT assays, assessed flow cytometry. Protein expression quantified...
DNA variants in APOL1 associate with kidney disease, but the pathophysiologic mechanisms remain incompletely understood. Model organisms lack gene, limiting degree to which disease states can be recapitulated. Here we present single-cell RNA sequencing (scRNA-seq) of genome-edited human organoids as a platform for profiling effects risk diverse nephron cell types.We performed footprint-free CRISPR-Cas9 genome editing induced pluripotent stem cells (iPSCs) knock high-risk G1 at native genomic...
Axin1 is a negative regulator of β-catenin signaling and its role in osteoblast precursor cells remains undefined. In the present studies, we determined changes postnatal bone growth by deletion analyzed newborn Axin1Osx mice found that hypertrophic cartilage area was largely expanded KO mice. A larger number chondrocytes unabsorbed matrix were marrow cavity Osteoclast formation metaphyseal subchondral areas significantly decreased, demonstrated decreased TRAP-positive cell numbers,...
Endothelial autocrine signaling is essential to maintain vascular homeostasis. There limited information about the role of endothelial in regulating severe pulmonary remodeling during onset arterial hypertension (PAH). In this study, we employed first (PH) mouse model, Egln1Tie2Cre (Tie2Cre-mediated disruption Egln1) mice, identify novel mediating cell (PVEC) proliferation and pathogenesis PAH. PVECs isolated from lung expressed upregulation many growth factors or angiocrine such as CXCL12,...