A5072286154- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Viral Infections and Outbreaks Research
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Insect symbiosis and bacterial influences
- Viral Infectious Diseases and Gene Expression in Insects
- Hepatitis B Virus Studies
- Transgenic Plants and Applications
- Bacteriophages and microbial interactions
- HIV Research and Treatment
- Virology and Viral Diseases
- Protein Kinase Regulation and GTPase Signaling
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Metabolism, Diabetes, and Cancer
- Glycosylation and Glycoproteins Research
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- Biosensors and Analytical Detection
- HIV/AIDS drug development and treatment
- Enzyme Structure and Function
- Endoplasmic Reticulum Stress and Disease
- Microbial Metabolic Engineering and Bioproduction
International Centre for Genetic Engineering and Biotechnology
2015-2024
Translational Health Science and Technology Institute
2015-2023
International Centre for Genetic Engineering and Biotechnology
2000-2022
Northern Health and Social Care Trust
2022
Centro de Ingeniería Genética y Biotecnología
2009-2020
Emory University
2015-2018
Birla Institute of Technology and Science - Hyderabad Campus
2013
Birla Institute of Technology and Science, Pilani
2013
University of Turku
2008-2010
Weatherford College
2008-2010
Abstract Background Pichia pastoris is an established eukaryotic host for the production of recombinant proteins. Most often, protein under control strong methanol-inducible aox1 promoter. However, detailed information about physiological alterations in P. accompanying shift from growth on glycerol to methanol-induced industrial relevant conditions missing. Here, we provide analysis response GS115 high-level Hepatitis B virus surface antigen (HBsAg). High product titers and retention...
The prevalence of diabetes is predicted to rise significantly in the coming decades. A recent analysis projects that by year 2030 there will be ~366 million diabetics around world, leading an increased demand for inexpensive insulin make this life-saving drug also affordable resource poor countries.A synthetic precursor (IP)-encoding gene, codon-optimized expression P. pastoris, was cloned frame with Saccharomyces cerevisiae alpha-factor secretory signal and integrated into genome pastoris...
Hepatitis B is a serious global public health concern. Though safe and efficacious recombinant vaccine available, its use in several resource-poor countries limited by cost. We have investigated the production of virus surface antigen (HBsAg) using yeast Pichia pastoris GS115 inserting HBsAg gene into alcohol oxidase 1 locus.Large-scale was optimized developing simple fed-batch process leading to enhanced product titers. Cells were first grown rapidly high-cell density batch defined medium...
Abstract Background A protective immune response against Hepatitis B infection can be obtained through the administration of a single viral polypeptide, surface antigen (HBsAg). Thus, vaccine is generated utilization recombinant DNA technology, preferentially by using yeast-based expression systems. However, polypeptide needs to assemble into spherical particles, so-called virus-like particles (VLPs), elicit required response. So far, no clear evidence has been presented showing whether...
Background Dengue is one of the fastest spreading vector-borne diseases, caused by four antigenically distinct dengue viruses (DENVs). Antibodies against DENVs are responsible for both protection as well pathogenesis. A vaccine that safe and efficacious in all people irrespective their age domicile still an unmet need. It becoming increasingly apparent design must eliminate epitopes implicated induction infection-enhancing antibodies. Methodology/principal findings We report a Pichia...
Background Dengue, a mosquito-borne viral disease, poses significant global public health risk. In tropical countries such as India where periodic dengue outbreaks can be correlated to the high prevalence of mosquito vector, circulation all four viruses (DENVs) and population density, drug for is being increasingly recognized an unmet need. Methodology/Principal findings Using knowledge traditional Indian medicine, Ayurveda, we developed systematic bioassay-guided screening approach explore...
There is currently no vaccine to prevent dengue (DEN) virus infection, which caused by any one of four closely related serotypes, DEN-1, DEN-2, DEN-3, or DEN-4. A DEN must be tetravalent, because immunity a single serotype does not offer cross-protection against the other serotypes. We have developed novel tetravalent chimeric protein fusing receptor-binding envelope domain III (EDIII) This was expressed in yeast, Pichia pastoris, and purified near homogeneity high yields. Antibodies induced...
Scatchard analysis of equilibrium dialysis studies have revealed that in the presence 3.0 mM MgCl2 and 150 KCl, calregulin has a single binding site for Ca2+ with an apparent dissociation constant (apparent Kd) 0.05 microM 14 sites Zn2+ Kd(Zn2+) 310 microM. to induces 5% increase intensity intrinsic fluorescence 2-3-nm blue shift emission maximum. causes dose-dependent about 250% its red maximum 11 nm. Half-maximal wavelength occurs at 0.4 mol Zn2+/mol calregulin, 100% is complete 2...
Dengue is a mosquito-borne viral disease with global prevalence. It caused by four closely-related dengue viruses (DENVs 1–4). A vaccine that can protect against all an unmet public health need. Live attenuated development efforts have encountered unexpected interactions between the viruses, raising safety concerns. This has emphasized need to explore non-replicating options. Virus-like particles (VLPs) which elicit robust immunity in absence of infection offer potential promise for...
Abstract Detection of hepatitis B Virus surface antigen (HBsAg) is an established method for diagnosing both acute and chronic virus (HBV) infection. In addition to enzyme immunoassays (EIAs), rapid diagnostic tests (RDTs) are available the detection HBsAg in resource-poor settings. However, RDTs have inadequate sensitivity therefore not suitable diagnosis patients with low levels blood screening. To provide a high-sensitivity RDT, we developed lateral flow immunoassay (LFIA) utilizing...
Abstract Background Dengue is a global public health problem for which no drug or vaccine available. Currently, there increasing interest in developing non-replicating dengue vaccines based on discrete antigenic domain of the major structural protein viruses (DENVs), known as envelope III (EDIII). The use bio-nanoparticles consisting recombinant viral polypeptides, better virus-like particles (VLPs), has emerged potential platform technology development. This work explores feasibility...