A5038564096- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Metal-Organic Frameworks: Synthesis and Applications
- Phytochemicals and Antioxidant Activities
- Metal complexes synthesis and properties
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Phytochemistry and Biological Activities
- Natural Antidiabetic Agents Studies
- Synthesis and biological activity
- Synthesis and Characterization of Heterocyclic Compounds
- Biodiesel Production and Applications
- Essential Oils and Antimicrobial Activity
- Analytical Methods in Pharmaceuticals
- Chemical Synthesis and Reactions
- Crystal structures of chemical compounds
- Inorganic and Organometallic Chemistry
- Electrochemical sensors and biosensors
- Advanced Nanomaterials in Catalysis
- Microbial Metabolism and Applications
- Nanoparticles: synthesis and applications
- Analytical Chemistry and Chromatography
- Click Chemistry and Applications
Government College University, Lahore
2015-2024
Universiti Malaysia Perlis
2024
Universitat Politècnica de València
2022
Nagano University
2022
Pancasila University
2022
Combined Military Hospital
2022
National University of Medical Sciences
2022
University of Lahore
2018-2021
COMSATS University Islamabad
2020
Bandung Institute of Technology
2019
Silver is a poisonous but precious heavy metal that has widespread application in various biomedical and environmental divisions. Wide-ranging usage of the twisted severe apprehensions. Henceforth there cumulative call for progress modest, low-cost and, ecological method remediation silver. In present study, Bacillus cereus was isolated from contaminated soil. Various experimental factors like amount AgNO3, inoculum size, temperature, time, pH were improved by using central composite design...
Abstract Background Pichia pastoris is an established eukaryotic host for the production of recombinant proteins. Most often, protein under control strong methanol-inducible aox1 promoter. However, detailed information about physiological alterations in P. accompanying shift from growth on glycerol to methanol-induced industrial relevant conditions missing. Here, we provide analysis response GS115 high-level Hepatitis B virus surface antigen (HBsAg). High product titers and retention...
The prevalence of diabetes is predicted to rise significantly in the coming decades. A recent analysis projects that by year 2030 there will be ~366 million diabetics around world, leading an increased demand for inexpensive insulin make this life-saving drug also affordable resource poor countries.A synthetic precursor (IP)-encoding gene, codon-optimized expression P. pastoris, was cloned frame with Saccharomyces cerevisiae alpha-factor secretory signal and integrated into genome pastoris...
Hepatitis B is a serious global public health concern. Though safe and efficacious recombinant vaccine available, its use in several resource-poor countries limited by cost. We have investigated the production of virus surface antigen (HBsAg) using yeast Pichia pastoris GS115 inserting HBsAg gene into alcohol oxidase 1 locus.Large-scale was optimized developing simple fed-batch process leading to enhanced product titers. Cells were first grown rapidly high-cell density batch defined medium...
Abstract Background A protective immune response against Hepatitis B infection can be obtained through the administration of a single viral polypeptide, surface antigen (HBsAg). Thus, vaccine is generated utilization recombinant DNA technology, preferentially by using yeast-based expression systems. However, polypeptide needs to assemble into spherical particles, so-called virus-like particles (VLPs), elicit required response. So far, no clear evidence has been presented showing whether...
The current study describes the emphatic use of response surface methodology for optimized biodiesel production using chemical and enzymatic transesterification rice bran sunflower oils. Optimal yields were determined to be 65.3 ± 2.0%, 73.4 3.5%, 96.5 1.6%, 89.3 2.0% 41.7 3.9% oil 65.6 1.2%, 82.1 1.7%, 92.5 2.8%, 72.6 1.6% 50.4 2.5% via catalyzed by NaOH, KOH NaOCH3,NOVOZYME-435 A.n. Lipase, respectively. Based upon analysis variance (ANOVA) Response Surface plots significant impact...
Ammonium perchlorate (AP) stands out as the predominant oxidizer in solid rocket propulsion systems, while sorbitol, frequently employed a fuel sounding rockets, is expected to generate non-hazardous gases during combustion. This study reports on effect of sorbitol potential organic green addition additive metals heat combustion, thermal properties and specific impulse for ammonium based propellant. The was measured using PROPEP 3.0 (Propellant evaluation program). Solid propellant...
The present work aimed to develop and validate a simple, rapid, sensitive, accurate, precise method for simultaneous determination of metformin hydrochloride vildagliptin in tablet biological samples. Isocratic elution both the analytes was performed at 35 °C by injecting 20 μL into Thermo Hypersil ODS C18 column (5 μm, 4.6 mm× 250 mm), while flow rate set 0.8 mL/min. mobile phase comprised methanol, acetonitrile, phosphate buffer (5:30:65, v / , pH 3.5), wavelength selected 212 nm. overall...
The consumer sensitivity toward application of synthetic colors led to exploitation food grade bio-colors from bacteria. Pseudomonas aeruginosa is an opportunistic, gram-negative bacteria that secrete a variety redox-active phenazine pigmented compounds, which are significant for biological activities. Pyocyanin, water soluble blue green pigment producing P. was screened aquatic habitats isolation Pakistan, identified and compared by 16S rRNA gene sequence genetic variability. similarity...
In this study, ZIF-67-based mixed matrix membrane was synthesized with a solution casting method using tetrahydrofuran as the solvent. The as-synthesized ZIF-67 characterized PXRD, TGA, ATR-FTIR, and BET analysis for surface area measurements. minimum 3 wt% loading of incorporated within hydrophobic polymer to evaluate CO2 adsorption performance ZIF-67. stability in pure water inorganic solvents investigated. maximum mixed-matrix (MMM) 0.5 mmol/g at 273 K, which is higher than that polymer....
oC and the fermentation pattern was studied. The maximum extracellular lipase activity of 7.3 U/mL intracellular 320 U/g mycelium were noted after 48 h. Although fungal biomass present at 13.6 g/L 60 h but highest specific growth rate observed between 6 18 in broth purified 134-folds with an overall yield 46% through purification procedure ammonium sulphate precipitation, ion exchange gel permeation chromatography. Km value enzyme for triolein hydrolysis found to be 1.229 m.mole/L.
Potential of sodium sulfide and sulfite, in the presence hydroxide was investigated to pretreat corncob (CC), bagasse (BG), water hyacinth rice husk (RH) for maximum digestibility. Response Surface Methodology employed optimization pretreatment factors such as temperature, time concentration Na₂S Na₂SO₃, which had high coefficient determination (R²) along with low probability value (P), indicating reliable predictability model. At optimized conditions, Na₂SO₃ remove up 97% lignin, from WH...
Diabetes mellitus type II (DMT-2) is a widely spread metabolic disorder both in developed and developing countries. The role of oxidative stress well established DMT-2 pathogenesis. synthetic drugs for are associated with serious side complications. Antioxidant α-glucosidase inhibitory actions phytochemicals from various plant species considered as an alternative to management. present study aimed evaluate the antioxidant activity, potential phytochemical profiling Hyophorbe lagenicaulis.The...