A5088615597- Influenza Virus Research Studies
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Viral Infections and Vectors
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- Immune cells in cancer
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- COVID-19 epidemiological studies
- Diabetes and associated disorders
- Neuroblastoma Research and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Zoonotic diseases and public health
- RNA and protein synthesis mechanisms
- Angiogenesis and VEGF in Cancer
- vaccines and immunoinformatics approaches
- Pneumonia and Respiratory Infections
- Viral Infections and Outbreaks Research
St. Jude Children's Research Hospital
2016-2025
Children's Research Hospital
2020-2024
Ducks Unlimited
2021-2023
University of Tennessee Health Science Center
2019-2023
Yale University
2023
Abbott (Sweden)
2023
University of Chile
2015-2020
Centers for Disease Control and Prevention
2005-2020
University of North Carolina at Chapel Hill
2017
UW Health University Hospital
2011
The Spanish influenza pandemic of 1918 to 1919 swept the globe and resulted in deaths at least 20 million people. basis pulmonary damage high lethality caused by H1N1 virus remains largely unknown. Recombinant viruses bearing hemagglutinin (HA) neuraminidase (NA) glycoproteins were rescued genetic background human A/Texas/36/91 (H1N1) (1918 HA/NA:Tx/91) virus. Pathogenesis experiments revealed that HA/NA:Tx/91 was lethal for BALB/c mice without prior adaptation is usually required A viruses....
Thrombospondin (TSP) forms specific complexes with transforming growth factor-beta (TGF-beta) in the alpha granule releasate of platelets and these TSP-TGF-beta inhibit bovine aortic endothelial cells (BAE). In studies, we report that TSP stripped associated TGF-beta (sTSP) retained inhibitory activity which was partially reversed by a neutralizing antibody for TGF-beta. Since BAE secrete latent TGF-beta, determined whether sTSP activates secreted cells. Cells were cultured or without then...
Transforming growth factor-beta (TGF-beta) is a potent regulatory protein secreted by virtually all cells in latent form. A major mechanism of regulating TGF-beta activity occurs through factors that control the processing to biologically active form molecule. We have shown previously thrombospondin 1 (TSP1), platelet alpha-granule and extracellular matrix protein, activates via protease- cell-independent localized binding/activation region type repeats TSP1. now report recombinant human...
G3BP1/2 are paralogous proteins that promote stress granule formation in response to cellular stresses, including viral infection. The nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) inhibits assembly and interacts with via an ITFG motif, residue F17, the N protein. Prior studies examining impact G3PB1-N interaction on SARS-CoV-2 replication have produced inconsistent findings, role this pathogenesis is unknown. Here, we use structural biochemical...
One of the primary points regulation transforming growth factor-β (TGF-β) activity is control its conversion from latent precursor to biologically active form. We have identified thrombospondin-1 as a major physiological regulator TGF-β activation. Activation dependent on interaction specific sequence in (K412RFK415) with complex. Platelet thrombospon-din-1 has and immunoreactive mature associated it. now report that latency-associated peptide (LAP) complex also interacts part...
Transforming growth factor-beta (TGF-beta) is a potent regulatory protein normally secreted by cells in latent form. Primary regulation of TGF-beta activity occurs through factors which control the processing to biologically active molecule. Thrombospondin (TSP), platelet alpha-granule and extracellular matrix protein, forms specific complexes with releasate activates endogenous endothelial via cell- protease-independent mechanism. In order better understand TSP-mediated activation...
Thrombospondin (TSP) was demonstrated to inhibit the growth of bovine aortic endothelial cells, an activity that not neutralized by antibodies TSP or other agents block TSP-cell interactions but partially reversed a neutralizing antibody transforming factor-beta (TGF-beta). Similar TGF-beta, supported NRK-49F colonies in soft agar dose-dependent manner, which required epidermal factor and anti-TGF-beta antibody. Chromatography preparation did separate TGF-beta-like NRK colony-forming from...
Thrombospondin (TSP) is complexed with transforming growth factor-beta (TGF-beta) in the alpha-granules of stimulated platelets. TSP stripped associated TGF-beta activity (sTSP) activates latent secreted by bovine aortic endothelial cells (BAE) culture. To better understand interactions TGF-beta, we investigated which region sTSP interacts TGF-beta. The chymotrypsin-resistant core TSP, contains procollagen-like and properdin-like type 1 repeats, activated both BAE a recombinant form small...
Transforming growth factor beta (TGF-beta) is a family of proteins secreted by virtually all cells in biologically inactive form. TGF-beta levels increase during many pathophysiological situations, including viral infection. The mechanism for increased activity infection not understood. We observed an active within 1 day mice infected with influenza virus. Further studies showed that the neuraminidase glycoprotein A and B viruses directly activates latent vitro. There are sufficient...
Obese adults have a greater risk of morbidity and mortality from infection with pandemic H1N1 influenza A virus (pH1N1). The objective the present study was to elucidate specific mechanisms by which obesity overweight impact cellular immune response pH1N1.Peripheral blood mononuclear cells healthy weight, overweight, obese individuals were stimulated ex vivo live pH1N1 then markers activation function measured using flow cytometry cytokine secretion cytometric bead array assays.CD4(+) CD8(+)...
ABSTRACT Influenza A viruses continue to cause widespread morbidity and mortality. There is an added concern that the highly pathogenic H5N1 influenza viruses, currently found throughout many parts of world, represent a serious public health threat may result in pandemic. Intervention strategies halt epidemic or pandemic are high priority, with emphasis on vaccines antiviral drugs. In these studies, we demonstrate 20-amino-acid peptide (EB, for entry blocker) derived from signal sequence...
Myelosuppression-related infections remain important causes of morbidity and mortality in children with acute lymphoblastic leukemia (ALL). By analyzing fecal samples collected at diagnosis after each the initial 3 phases chemotherapy, we evaluated role gut microbiota predicting 199 newly diagnosed ALL. The bacterial 16S rRNA gene was analyzed by high-depth sequencing to determine diversity composition microbiome. After induction reinduction I microbial decreased significantly relative...
Transforming growth factor-beta (TGF-β), a multifunctional cytokine regulating several immunologic processes, is expressed by virtually all cells as biologically inactive molecule termed latent TGF-β (LTGF-β). We have previously shown that activity increases during influenza virus infection in mice and suggested the neuraminidase (NA) protein mediates this activation. In current study, we determined mechanism of activation LTGF-β NA from A/Gray Teal/Australia/2/1979 mobility shift enzyme...