Sidharth Mahapatra
A5053360052- Glioma Diagnosis and Treatment
- Respiratory Support and Mechanisms
- MicroRNA in disease regulation
- Brain Metastases and Treatment
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- Respiratory viral infections research
- Lung Cancer Research Studies
- Cancer Mechanisms and Therapy
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Sepsis Diagnosis and Treatment
- Neonatal Respiratory Health Research
- Ferroptosis and cancer prognosis
- Nerve injury and regeneration
- Neuroblastoma Research and Treatments
- RNA Interference and Gene Delivery
- Peptidase Inhibition and Analysis
- Alzheimer's disease research and treatments
- Protein Degradation and Inhibitors
- Radiopharmaceutical Chemistry and Applications
- Advanced Glycation End Products research
- Pancreatic and Hepatic Oncology Research
- Glycosylation and Glycoproteins Research
University of Nebraska Medical Center
2016-2025
American Federation for Medical Research
2024
Children's Hospital & Medical Center
2019-2024
Nebraska Medical Center
2019-2023
Lucile Packard Children's Hospital
2010-2014
Stanford University
2011
Rosalind Franklin University of Medicine and Science
2007-2009
Abstract Background Small cell lung cancer (SCLC) is an aggressive subtype that associated with high recurrence and poor prognosis. Due to lack of potential drug targets, SCLC patients have few therapeutic options. MicroRNAs (miRNAs) provide interesting repertoire molecules; however, the identification miRNAs regulating growth metastasis their precise regulatory mechanisms are not well understood. Methods To identify novel SCLC, we performed miRNA-sequencing from donor/patient serum samples...
<h3>Importance</h3> Hyperosmolar agents are cornerstone therapies for pediatric severe traumatic brain injury. Guideline recommendations 3% hypertonic saline (HTS) based on limited numbers of patients, and no study to date has supported a recommendation mannitol. <h3>Objectives</h3> To characterize current use hyperosmolar in injury assess whether HTS or mannitol is associated with greater decreases intracranial pressure (ICP) and/or increases cerebral perfusion (CPP). <h3>Design, Setting,...
Of the four primary subgroups of medulloblastoma, most frequent cytogenetic abnormality, i17q, distinguishes Groups 3 and 4 which carry highest mortality; haploinsufficiency 17p13.3 is a marker for particularly poor prognosis. At terminal end this locus lies miR-1253, brain-enriched microRNA that regulates bone morphogenic proteins during cerebellar development. We hypothesized miR-1253 confers novel tumor-suppressive properties in medulloblastoma. Using two different cohorts medulloblastoma...
Coinfection with influenza virus and methicillin-resistant Staphylococcus aureus (MRSA) causes life-threatening necrotizing pneumonia in children. Sporadic incidence precludes evaluation of antimicrobial efficacy. We assessed the clinical characteristics outcomes critically ill children influenza-MRSA evaluated antibiotic use.We enrolled (<18 years) infection respiratory failure across 34 pediatric intensive care units 11/2008-5/2016. compared baseline characteristics, courses, therapies...
<h3>Importance</h3> Diversion of cerebrospinal fluid (CSF) has been used for decades as a treatment children with severe traumatic brain injury (TBI) and is recommended by evidenced-based guidelines. However, these recommendations are based on limited studies. <h3>Objective</h3> To determine whether CSF diversion associated improved Glasgow Outcome Score–Extended Pediatrics (GOS-EP) decreased intracranial pressure (ICP) in TBI. <h3>Design, Setting, Participants</h3> This observational...
Cancer stem cells (CSCs), which mediate drug resistance and disease recurrence in several cancers, are therapeutically relevant to ovarian cancer (OC), wherein approximately 80% of patients manifest with tumor recurrence. While there markers for CSCs (OCSCs), the mechanism their self-renewal maintenance by unique driver/markers is poorly understood. Here, we evaluated role hPaf1/PD2, a core component RNA Polymerase II-Associated Factor (PAF) complex, OCSCs through marker functional analyses,...
Abstract Purpose: Breast cancer (BC) brain metastasis (BrM) remains a significant clinical problem. Mucins have been implicated in metastasis; however, if they are also involved BCBrM unknown. We queried BrM patient databases and found Mucin 5AC (MUC5AC) to be upregulated therefore sought define the role of MUC5AC BCBrM. Experimental design: In-silico dataset analysis, RNA-sequence profiling on patients cell lines, analysis patients’ serum samples, in-vitro/vivo knockdown experiments were...
<p>Supplementary Figure 2. MUC5AC IHC staining using Aperio Digital Imaging revealed strong to medium scores in BrM patient samples. Tumor areas were defined by annotations (green color) and necrotic or not corresponding tumor excluded as negative (blue color). Pixel Positive algorithm was with 4 scales: 1. Negative (blue), Weak positive (yellow), 3. Medium (orange) 4. Strong (red). Controls for development DAB. A. Annotation (tumor area) tissue of pancreas cancer without antibody...
<p>Supplementary Figure 3. MUC5AC promotes brain metastasis. A. Western blot for HER2 expression in MDA-231BR cells. B. showing knockout of the HCC1954BR C-F. Wound healing assay and quantification reduced migration shMUC5AC cells (C D) KO (E F). Representative images (G) (H) trans-endothelial cell SCR (HCC1954BR). I. Schematic representation 3D ex-vivo culture J. fluorescence microscopy slices (organotypic culture) GFP+ MDA-231P, invasion into tissue. Data presented as mean ± SD from...
<p>Supplementary Figure 6. PLB1001 inhibit cMET and CD44v6 expression. A. Representative IHC images showing MUC5AC expression in G5-3 PDXs B. fluorescence microscopy GFP+ C. of MUC5AC, cMET, cleaved caspase3 PDX treated with vehicle or (50mg/kg).</p>
<p>Supplementary Figure 5. MUC5AC silencing inhibits cMET and CD44v6 expression Astrocytes induce in breast cancer cells. A-B. Western blots showing knockdown of MDA-231BR HER2 (A) knockout HCC1954BR (B) reduces CD44v6. C. IHC staining for on brain tissue sections from mice injected with shSCR shMUC5AC D. SKBR3 cells were treated human astrocyte conditioned media 24h lysed subjected expression. HGF induced signaling (E) (F); (100ng/mL) indicated times. After treatment p-cMET using...
<div>AbstractPurpose:<p>Breast cancer brain metastasis remains a significant clinical problem. Mucins have been implicated in metastasis; however, whether they are also involved breast unknown. We queried databases of patients with and found mucin 5AC (MUC5AC) to be upregulated therefore sought define the role MUC5AC metastasis.</p>Experimental Design:<p><i>In silico</i> dataset analysis, RNA-sequence profiling patient samples cell lines, analysis serum...
<p>Supplementary Figure 1. Expression of MUC5AC is high in breast cancer brain metastasis. A. Heatmap showing the expression upregulated genes BC BrM cells as compared to primary (MDA-231BR vs MDA-231P). B. Comparison (z-score) between and metastasis samples, using online available Gene Omnibus (GEO) public datasets, probe (214385_s_at). C. goblet marker cell lines Data represents upregulation proteins. D. Pie chart representing H-score for tissues originating from (Breast cancer), LC...
<p>Supplementary Figure 4. MUC5AC silencing inhibits brain metastasis. A. Fluorescence microscopy images showing Cytokeratin 8/18 and in the tissue sections of mice intracardially injected with shSCR shMUC5AC KD MDA-231BR HER2 cells. B. The IHC show expression cMET. C. H-score quantification cMET different cohorts BC BrM patients. D. CD44v6. E. Clustering gene interaction network analysis (http://string-db.org/) shows MUC5AC, CD44v6 interaction.</p>
Abstract Background MYC amplification or overexpression is common in Group 3 medulloblastoma and associated with the worst prognosis. Recently, protein arginine methyl transferase (PRMT) 5 expression has been closely aberrant function various cancers, including brain tumors such as glioblastoma. However, role of PRMT5 its association have not explored. Here, we report a novel regulator implicate potential therapeutic target MYC-driven medulloblastoma. Methods Expression between primary were...
Background Influenza virus is responsible for a large global burden of disease, especially in children. Multiple Organ Dysfunction Syndrome (MODS) life-threatening and fatal complication severe influenza infection. Methods We measured RNA expression 469 biologically plausible candidate genes children admitted to North American pediatric intensive care units with infection without MODS. Whole blood samples from 191 influenza-infected (median age 6.4 years, IQR: 2.2, 11) were collected median...