A5042835626- Burkholderia infections and melioidosis
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Immune cells in cancer
- Plant Pathogenic Bacteria Studies
- Gut microbiota and health
- Brucella: diagnosis, epidemiology, treatment
- Cancer Research and Treatments
- Acute Myeloid Leukemia Research
- Cancer, Hypoxia, and Metabolism
- Genomics and Rare Diseases
- Chronic Lymphocytic Leukemia Research
- Genomics and Chromatin Dynamics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer-related gene regulation
- Galectins and Cancer Biology
- Bacterial biofilms and quorum sensing
- Genetic and Kidney Cyst Diseases
- HER2/EGFR in Cancer Research
- Renal and related cancers
- Barrier Structure and Function Studies
- Cancer Immunotherapy and Biomarkers
- Chemical Looping and Thermochemical Processes
- Renal cell carcinoma treatment
National Cancer Institute
2020-2024
Center for Cancer Research
2020-2024
USA Mitchell Cancer Institute
2016-2024
University of South Alabama
2014-2024
Northern Arizona University
2012-2016
During routine screening for
Lipopolysaccharide (LPS) is one of the most important virulence and antigenic components Burkholderia pseudomallei, causative agent melioidosis. LPS diversity in B. pseudomallei has been described as typical, atypical or rough, based upon banding patterns on SDS-PAGE. Here, we studied genetic molecular basis these phenotypic differences. Bioinformatics was used to determine genes known predicted be involved biosynthesis O-antigenic moiety its near-relative species. Multiplex-PCR assays were...
Abstract Change within the intratumoral microbiome is a common feature in lung and other cancers may influence inflammation immunity tumor microenvironment, affecting growth metastases. We previously characterized cancer patients identified Acidovorax temperans as enriched tumors. Here, we instilled A. an animal model driven by mutant K-ras Tp53. This revealed accelerates development burden through infiltration of proinflammatory cells. Neutrophils exposed to displayed mature,...
While dysregulation of RNA splicing has been recognized as an emerging target for cancer therapy, the functional significance and individual factors in brain tumors is poorly understood. Here, we identify SON a master regulator that activates PTBP1-mediated oncogenic while suppressing RBFOX2-mediated non-oncogenic neuronal glioblastoma multiforme (GBM). overexpressed GBM patients knockdown causes failure intron removal from PTBP1 transcript, resulting downregulation inhibition its downstream...
The pangenomic diversity in Burkholderia pseudomallei is high, with approximately 5.8% of the genome consisting genomic islands. Genomic islands are known hotspots for recombination driven primarily by site-specific associated tRNAs. However, rates other portions also a feature we expected to disrupt gene order. We analyzed pangenome 37 isolates B. and demonstrate that 'open', 136 new genes identified each sequenced, global core consists 4568±16 homologs. Genes metabolism were statistically...
The study of the tumor microbiome has been garnering increased attention. We developed a computational pipeline (CSI-Microbes) for identifying microbial reads from single-cell RNA sequencing (scRNA-seq) data and analyzing differential abundance taxa. Using series controlled experiments analyses, we performed first systematic evaluation efficacy recovering unique molecular identifiers by multiple scRNA-seq technologies, which identified newer 10x chemistries (3' v3 5') as best suited...
Abstract Background Burkholderia pseudomallei is the etiological agent of melioidosis and a CDC category B select with no available effective vaccine. Previous immunizations in mice have utilized lipopolysaccharide (LPS) as potential vaccine target because it known one most important antigenic epitopes . Complicating this strategy are four different B. LPS O-antigen types: A, B, B2, rough. Sero-crossreactivity common among O-antigens species. Here, we identified presence multiple types...
Emerging evidence has shown that active enhancers are abundantly transcribed, generating long non-coding RNAs, called enhancer RNAs (eRNAs). While putative eRNAs often observed from RNA sequencing, the roles of most remain largely unknown. Previously, we identified regions at MALAT1 locus form chromatin-chromatin interactions under hypoxia, and one these is located about 30 kb downstream NEAT1 gene -20 upstream ( –20 enhancer). Here, report a novel eRNA, named e NE AT1 - MA LAT1 L ocus...
We describe the complete genome sequence of Burkholderia pseudomallei MSHR305, a clinical isolate taken from fatal encephalomyelitis case, rare form melioidosis. This will be used for comparisons to identify genes that are involved in neurological cases.
Here, we describe the draft genome sequence of Burkholderia pseudomallei NCTC 13392. This isolate has been distributed as K96243, but distinct genomic differences have identified. The this will provide context for previously conducted functional studies.
Abstract Burkholderia pseudomallei is the causative agent of melioidosis and endemic to Southeast Asia northern Australia. There no available vaccine accurate diagnosis difficult, time-consuming labor intensive. Early an important part successful treatment current serological tests are inadequate based upon multiple antigens. Identifying specific immunogenic proteins which highly seroreactive may yield potential diagnostic targets for detecting antibodies antigens melioidosis. We have used...
Abstract The study of the tumor microbiome has been garnering increased attention. We developed a computational pipeline (CSI-Microbes) for identifying microbial reads from single-cell RNA sequencing (scRNA-seq) data. Using series controlled experiments and analyses, we performed first systematic evaluation efficacy recovering UMIs by multiple scRNA-seq technologies, which identified newer 10x chemistries (3’ v3 5’) as best suited approach. Based on these findings, analyzed patient...
Abstract Cellular growth and metabolism are tightly regulated by nutrient availability, yet cancer cells frequently lose these controls, enabling unchecked proliferation even in the absence of sufficient nutrients. While non-coding RNAs known to regulate metabolic enzyme expression, their direct role nutrient-sensing pathways remains unclear. MALAT1-associated small cytoplasmic RNA (mascRNA) is a 61-nucleotide with tRNA-like structure, cleaved from 3’ end long MALAT1. mascRNA has been shown...
Abstract Lung cancer is the most common cause of cancer-related death worldwide in both men and women. The overall survival lung remains poor due to limitations diagnostic therapeutic strategies. Therefore, development novel biomarkers urgently needed improve patient prognosis. Growing evidence has demonstrated alterations normal microbiome cancer. Dysbiosis may alter microbiota-host interactions induce immune dysregulation, therefore contribute promotion tumorigenesis. Although multiple...
Abstract Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a long non-coding RNA overexpressed in many solid tumors such as breast, colorectal, liver, lung, and pancreatic. Its expression progressively increases, first comparison of adjacent normal tissue to the primary tumor again from secondary metastatic sites. It has been reported that MALAT1 controls gene through regulation alternative mRNA splicing well chromatin binding histone modification. These processes result...
Abstract SON is a poorly characterized nuclear protein that particularly abundant in hematopoietic cells/organs and embryonic stem cells. This was recently identified as splicing co-factor required for proper cell cycle progression maintenance of pluripotency. Although SON's function RNA highlighted, originally DNA-binding potential regulator transcription. However, the mechanism by controls transcription its disease relevance are completely unknown. To investigate genome-wide gene...