A5026970161- Congenital heart defects research
- Pluripotent Stem Cells Research
- Renal and related cancers
- Pregnancy and preeclampsia studies
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Angiogenesis and VEGF in Cancer
- Tissue Engineering and Regenerative Medicine
- Neonatal Respiratory Health Research
- Mesenchymal stem cell research
- RNA modifications and cancer
- Hemoglobinopathies and Related Disorders
- Fungal and yeast genetics research
- Reproductive System and Pregnancy
- Connective tissue disorders research
- CRISPR and Genetic Engineering
- Apelin-related biomedical research
- Congenital Diaphragmatic Hernia Studies
- MicroRNA in disease regulation
- Signaling Pathways in Disease
- Proteoglycans and glycosaminoglycans research
- Cancer Cells and Metastasis
University of Wisconsin–Madison
2011-2022
Karen Hospital
2015
University of Oxford
1991-1995
University of California, San Francisco
1989
University of Illinois Chicago
1980-1985
ABSTRACT We describe and illustrate a set of morphological landmarks for classifying mouse embryos by gross morphology from before gastrulation to the beginning organogenesis. These are visible at resolution dissecting microscope in that intact except reflexion Reichert’s membrane. Adoption these criteria should facilitate interpretation both expression patterns genes consequences experimental manipulation during early postimplantation development.
We have defined the histone acetylation pattern of endogenous murine β-globin domain, which contains erythroidspecific genes. The locus control region (LCR) and transcriptionally active promoters were enriched in acetylated histones fetal liver relative to brain, whereas inactive hypoacetylated. In contrast, LCR both hyperacetylated yolk sac. Hypersensitive site two was also embryonic stem cells, Thus, varied at different developmental stages. Histone deacetylase inhibition selectively...
The chorio-allantoic placenta forms through the fusion of allantois (progenitor tissue umbilical cord), with chorionic plate. murine contains high levels hematopoietic stem cells, and is therefore a cell niche. However, it not known whether site emergence, or cells originate from other sites in conceptus then colonize placenta. Here, we show that chorion, isolated prior to establishment circulation, have potential give rise myeloid definitive erythroid following explant culture. We further...
myc and N-myc are related genes whose similar protein products may be used for different purposes in vertebrate organisms. We have explored this possibility by using hybridization situ to examine the expression of during gastrulation mouse embryos. Throughout gastrulation, RNA was most abundant extraembryonic cells; contrast, found at highest levels expanding primitive streak other portions embryonic mesoderm. Differentiation mesoderm epithelioid cells accompanied diminished N-myc....
The LIM domain-binding protein 1 (Ldb1) is found in multi-protein complexes containing various combinations of LIM-homeodomain, LIM-only, bHLH, GATA and Otx transcription factors. These proteins exert key functions during embryogenesis. Here we show that targeted deletion the Ldb1 gene mice results a pleiotropic phenotype. There no heart anlage head structures are truncated anterior to hindbrain. In about 40% mutants, posterior axis duplication observed. also severe defects mesoderm-derived...
At the blastocyst stage of pre-implantation mouse development, close contact polar trophectoderm with inner cell mass (ICM) promotes proliferation undifferentiated diploid trophoblast. However, ICM/polar intimacy is not maintained during post-implantation raising question how growth trophoblast controlled this time. The search for cellular basis in development was addressed an vitro spatial and temporal analysis fibroblast factor 4-dependent stem potential. Two derivatives – early-streak...
ABSTRACT The aim of this study was to determine whether the blood vessels murine allantois are formed by vasculogenesis or angiogenesis. Morphological analysis revealed that differentiation allantoic mesoderm into an outer layer mesothelium and inner vascular network begins in distal region allantois, which is most remote from other tissues, as early late neural plate stage (approximately 7.75 days postcoitum). Nascent were not found base until 4-somite pairs had fetus 8.25 postcoitum),...
Erythroid Krüppel-like factor (EKLF) is an erythroid cell-specific transcription that mediates activation via binding to a 9 base pair sequence encompasses the CACCC element, one of trio evolutionarily conserved motifs are functionally important for red genes. Molecular analyses have delineated specificity its interaction and through CAC site at adult beta-globin promoter. However, expression distribution during murine ontogeny not been established. To address these issues, we focused on...
The murine allantois is the future umbilical component of placenta. base also thought to contain germ line. We have examined fate and developmental potency cells within during gastrulation. lacZ-expressing headfold-stage allantoises (approximately 8.0 days postcoitum; dpc) were subdivided into three proximodistal regions transplanted sites in synchronous non-transgenic host embryos: primitive streak at level prospective paraxial mesoderm, lateral plate allantois. After 23 hours culture,...
Abstract Oct‐3/4 was localized to the mouse conceptus between onset of gastrulation and 16‐somite pairs (‐s; ∼6.5–9.25 days postcoitum, dpc). Results revealed in a continuum morphologically distinct epiblast‐derived embryonic extraembryonic tissues. In allantois, distal‐to‐proximal diminution domain over time co‐localization with Flk‐1 angioblasts accorded role vascular differentiation presence stem cell reservoir. addition, visceral endoderm exhibited dynamic salt‐and‐pepper distribution,...
ABSTRACT Culture of postimplantation conceptuses was used in conjunction with microsurgery to investigate the timing, mechanism and developmental regulation chorioallantoic fusion mouse. The timing determined both freshly recovered those that had been cultured from as early mid-streak stage. Attachment allantois chorion found have occurred most by 6-somite stage, irrespective whether they cultured. In investigating fusion, we wished determine it depended on directed growth allantoic bud or...
Mouse conceptuses homozygous for mutations in brachyury (T)exhibit a short, misshapen allantois that fails to fuse with the chorion. Ultimately, mutant embryos die during mid-gestation. In 60 years since this discovery, role of T allantoic development has remained obscure. protein was recently identified several new sites mouse gastrulation, including core allantois, where its function is not known. Here, using molecular, genetic and classical techniques embryology,we have investigated...
Abstract The whereabouts and properties of the posterior end primitive streak have not been identified in any species. In mouse, streak's terminus is assumed to be confined embryonic compartment, give rise allantois, which links embryo its mother during pregnancy. this study, we refined our understanding biology murine relation allantois. Through a combination immunostaining morphology, demonstrate that spans extraembryonic regions at onset neural plate stage (∼7.0 days postcoitum, dpc)....
Abstract While much progress has been made in the resolution of cellular hierarchy underlying cardiogenesis, our understanding chamber-specific myocardium differentiation remains incomplete. To better understand ventricular differentiation, we targeted ventricle-specific gene, Irx4, mouse embryonic stem cells to generate a reporter cell line. Using an antibiotic-selection approach, purified Irx4+ vitro from differentiating embryoid bodies. The isolated proved be highly proliferative and...
Hypoblast/visceral endoderm assists in amniote nutrition, axial positioning and formation of the gut. Here, we provide evidence, currently limited to humans non-human primates, that hypoblast is a purveyor extraembryonic mesoderm mouse gastrula. Fate mapping unique segment visceral associated with allantoic component primitive streak, referred as "AX", revealed supplies placentae mesoderm. Exfoliation AX was dependent upon contact which modulated Hedgehog signaling. Resolution AX's...
Despite its importance as the source of one three major vascular systems in mammalian conceptus, little is known about murine allantois, which will become umbilical cord chorio-allantoic placenta. During gastrulation, allantois grows into exocoelomic cavity a mesodermal extension posterior primitive streak. On basis morphology, gene expression and/or function, cell types have been identified allantois: an outer layer mesothelial cells, whose distal portion transformed chorio-adhesive and...