A5076425450- Pluripotent Stem Cells Research
- Hematopoietic Stem Cell Transplantation
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- CAR-T cell therapy research
- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Neutropenia and Cancer Infections
- Vascular Anomalies and Treatments
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- Phagocytosis and Immune Regulation
- Pulmonary Hypertension Research and Treatments
- Tracheal and airway disorders
- Sepsis Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Pregnancy and preeclampsia studies
- Skin Protection and Aging
- Hair Growth and Disorders
- Brain Metastases and Treatment
- Cytomegalovirus and herpesvirus research
- Reproductive System and Pregnancy
- Transplantation: Methods and Outcomes
- Insect Resistance and Genetics
Harvard Stem Cell Institute
2021-2024
Dana-Farber Cancer Institute
2021-2024
Boston Children's Hospital
2019-2024
Harvard University
2021-2024
Howard Hughes Medical Institute
2018-2024
Peking University
2015-2023
Peking University People's Hospital
2015-2023
National Clinical Research Center for Digestive Diseases
2023
Peking University Cancer Hospital
2022
University of Wisconsin–Madison
2015-2019
Abstract P‐glycoprotein [P‐gp or the ATP‐binding cassette transporter B1 (ABCB1)] is an important participant in multidrug resistance of cancer cells, yet precise function this arthropod unknown. The aim study was to determine importance P‐gp for susceptibility insecticides beet armyworm ( Spodoptera exigua ) using clustered regularly interspaced short palindromic repeats/CRISPR‐associated 9 (CRISPR/Cas9) gene‐editing technology. We cloned open reading frame (ORF) encoding S. protein...
Human pluripotent stem cell (hPSC)-derived endothelial cells and their progenitors are important for vascular research therapeutic revascularization. Here, we report a completely defined progenitor differentiation platform that uses minimalistic medium consisting of Dulbecco's modified eagle ascorbic acid, lacking albumin growth factors. Following hPSC treatment with GSK-3β inhibitor culture in this medium, protocol generates more than 30% multipotent CD34+ CD31+ can be purified to >95% via...
Adult stem cells are responsible for life-long tissue maintenance. They reside in and interact with specialized microenvironments (niches). Using murine hair follicle as a model, we show that when junctional perturbations the niche disrupt barrier function, adjacent dramatically change their transcriptome independent of bacterial invasion become capable directly signaling to recruiting immune cells. Additionally, these elevate cell cycle transcripts which reduce quiescence threshold,...
The hematopoietic niche is a supportive microenvironment composed of distinct cell types, including specialized vascular endothelial cells that directly interact with stem and progenitor (HSPCs). molecular factors specify orchestrate HSPC homeostasis remain largely unknown. Using multi-dimensional gene expression chromatin accessibility analyses in zebrafish, we define conserved signature cis-regulatory landscape are unique to sinusoidal the niche. enhancer mutagenesis transcription factor...
Abstract Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) exhibit a fetal phenotype that limits in vitro and therapeutic applications. Strategies to promote cardiomyocyte maturation have focused interventions on differentiated hPSC-CMs, but this study tests priming of early cardiac progenitor cells (CPCs) with polyinosinic-polycytidylic acid (pIC) accelerate maturation. CPCs were from hPSCs using monolayer differentiation protocol defined small molecule Wnt temporal modulation,...
Abstract During heart development, epicardial progenitors contribute various cardiac lineages including smooth muscle cells, fibroblasts, and endothelial cells. However, their specific contribution to the human endothelium has not yet been resolved, at least in part due inability expand maintain primary or pluripotent stem cell (hPSC)‐derived Here we first generated CDH5‐2A‐eGFP knock‐in hPSC lines differentiated them into self‐renewing WT1+ which gave rise cells upon VEGF treatment vitro ....
Abstract While much progress has been made in the resolution of cellular hierarchy underlying cardiogenesis, our understanding chamber-specific myocardium differentiation remains incomplete. To better understand ventricular differentiation, we targeted ventricle-specific gene, Irx4, mouse embryonic stem cells to generate a reporter cell line. Using an antibiotic-selection approach, purified Irx4+ vitro from differentiating embryoid bodies. The isolated proved be highly proliferative and...
Abstract Background Cytomegalovirus ( CMV ) enteritis after allogeneic hematopoietic stem cell transplantation (allo‐ HSCT is difficult to diagnose. We aimed evaluate the sensitivity and specificity of detection DNA in feces for predicting enteritis. Methods patients with intestinal graft‐versus‐host disease GVHD were enrolled if they met following criteria: (i) underwent a colonoscopy (ii) peripheral blood specimens available within 24 h colonoscopy. The histology was used as gold standard...
Abstract Stress granules (SGs) are crucial in RNA regulation, affecting cell fate and function. SGs contain RNAs, some of which can be methylated. We studied m 6 A modifications during the human CD34 + HSPCs (hCD34 ) differentiating into erythroid cells found that mRNAs encoding many erythroid-specific proteins had decreased methylation differentiation. Increased levels ALKBH5 demethylase erythropoiesis controls 3’UTR these mRNAs. hCD34 carrying mutations demonstrated a block erythropoiesis,...
The National Heart, Lung, and Blood Institute Progenitor Cell Translational Consortium Meeting was hosted virtually on November 5, 2020, with 93 attendees across 20 research groups. purpose of this meeting to exchange recent findings, discuss current efforts, identify prospective opportunities in the field hematopoietic stem progenitor cell therapeutic discovery.
Abstract The hematopoietic niche is a supportive microenvironment comprised of distinct cell types, including specialized vascular endothelial cells that directly interact with stem and progenitor (HSPCs). molecular factors specify orchestrate HSPC homeostasis remain largely unknown. Using multi-dimensional gene expression chromatin accessibility analyses, we define conserved signature cis -regulatory landscape unique to sinusoidal in the niche. enhancer mutagenesis transcription factor...
While human pluripotent stem cell-derived cardiomyocytes have shown promise in engrafting and maturing animals following myocardial infarction, transplanted cells largely exist as histologically self-contained blocks of with moderate or undetectable functional benefit. We therefore sought to develop methods isolate a proliferative progenitor capable differentiating both ventricular supportive cardiac mesenchymal cells, these may hold for regenerative medicine. To progenitor, fluorescent...
Topic: 22. Stem cell transplantation - Clinical Background: Bronchiolitis obliterans syndrome (BOS) is a common and potentially devastating non-infectious pulmonary complication following allogeneic hematopoietic stem (allo-HSCT). Although BOS rare, affecting only 5% to 12% of HSCT recipients, it significant problem after because the high attributed morbidity mortality. Early treatment, before structural irreversible changes have occurred, crucial reduce disease some advances occurred in...
Abstract Background Pulmonary arteriovenous malformation (PAVM) is an abnormal communication between pulmonary vasculatures and has unclear boundary with surrounding lung tissues. At present, surgeons can only determine its location by preoperative imaging intraoperative palpation, despite soft texture. Indocyanine green(ICG), a near-infrared fluorophore, been demonstrated useful in the accurate identification of vascular tissue. Therefore, we explored application PAVM cases. Case...
Objective: To investigate the potential of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in mitigating adverse prognosis associated with central nervous system leukemia (CNSL) and to assess significance prophylactic intrathecal injection. Methods: A retrospective cohort analysis was conducted involving 30 patients acute who had a history CNSL underwent allo-HSCT at Peking University People's Hospital between September 2012 March 2018 (referred as CNSL-positive group). In...
Cardiomyocytes derived from human induced pluripotent stem cells (hiPS-CMs) hold promise for disease modeling, drug discovery, and therapy, but the challenge remains to create mature cardiomyocytes like those found in adult heart. While groups have increased maturity of hiPS-CMs extended culture with electrical, metabolic, mechanical stimulation, we hypothesized that epigenetic modulation during formation cardiac progenitors (hiPS-CPCs) could enhance their capacity form CMs. We priming...