A5058840415- Zebrafish Biomedical Research Applications
- Hematopoietic Stem Cell Transplantation
- T-cell and B-cell Immunology
- Aquaculture disease management and microbiota
- Immune Cell Function and Interaction
- Prostate Cancer Treatment and Research
- Advanced Graph Theory Research
- Inflammatory Bowel Disease
- Computational Geometry and Mesh Generation
- Single-cell and spatial transcriptomics
- Gastrointestinal Bleeding Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Pluripotent Stem Cells Research
- Diverticular Disease and Complications
- Complexity and Algorithms in Graphs
- Mathematical Dynamics and Fractals
- Computability, Logic, AI Algorithms
- Biosensors and Analytical Detection
- Immune cells in cancer
- Dysphagia Assessment and Management
- Microscopic Colitis
- Cardiomyopathy and Myosin Studies
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Cardiac Valve Diseases and Treatments
Royal Victoria Regional Health Centre
2025
Cleveland Clinic
2024
Lucile Packard Children's Hospital
2024
Donald & Barbara Zucker School of Medicine at Hofstra/Northwell
2024
Feinstein Institute for Medical Research
2024
Essex Cardiothoracic Centre
2021-2023
Howard Hughes Medical Institute
2016-2023
Boston Children's Hospital
2014-2023
Dana-Farber Cancer Institute
2017-2023
Harvard Stem Cell Institute
2017-2023
Androgen receptor (AR) signaling is a key driver of prostate cancer, and androgen-deprivation therapy (ADT) standard treatment for patients with advanced metastatic disease. However, receiving ADT eventually develop incurable castration-resistant cancer (CRPC). Here, we report that the chromatin modifier LSD1, an important regulator AR transcriptional activity, undergoes epigenetic reprogramming in CRPC. LSD1 this setting activated subset cell-cycle genes, including CENPE, centromere binding...
Abstract Tunneling nanotubes (TNTs) are actin-based membranous structures bridging distant cells for intercellular communication. We define roles TNTs in stress adaptation and treatment resistance prostate cancer (PCa). Androgen receptor (AR) blockade metabolic induce TNTs, but not normal prostatic epithelial or osteoblast cells. Co-culture assays reveal enhanced TNT formation between stressed unstressed PCa as well from to osteoblasts. Stress-induced chaperones clusterin YB-1 localize...
Natural killer (NK) cells recognize and eliminate infected malignant cells. Their life histories are poorly understood, particularly in humans, due to lack of informative models endogenous clonal markers. Here, we apply transplantation barcoded rhesus macaque hematopoietic interrogate the landscape NK cell production, expansion, at a level long term after proliferative challenge. We identify oligoclonal populations CD56-CD16+ that characterized by marked expansions contractions over time yet...
We present a clinical case highlighting high-definition intravascular ultrasound (HD-IVUS)-guided tip detection-antegrade dissection and re-entry (ADR). This novel technique in percutaneous coronary intervention of chronic total occlusions was pioneered Japan using specially designed catheter (AnteOwl WR IVUS, Terumo) replicated this off-the-shelf equipment after antegrade retrograde attempts with conventional methods were unsuccessful. To the best our knowledge, marks first successful...
The microenvironment is an important regulator of hematopoietic stem and progenitor cell (HSPC) biology. Recent advances marking fluorescent HSPCs have allowed exquisite visualization in the caudal tissue (CHT) developing zebrafish. Here, we show that chemokine cxcl8 its receptor, cxcr1, are expressed by zebrafish endothelial cells, identify cxcl8/cxcr1 signaling as a positive HSPC colonization. Single-cell tracking experiments demonstrated this result increases HSPC–endothelial “cuddling,”...
The hematopoietic niche is a supportive microenvironment composed of distinct cell types, including specialized vascular endothelial cells that directly interact with stem and progenitor (HSPCs). molecular factors specify orchestrate HSPC homeostasis remain largely unknown. Using multi-dimensional gene expression chromatin accessibility analyses in zebrafish, we define conserved signature cis-regulatory landscape are unique to sinusoidal the niche. enhancer mutagenesis transcription factor...
The geographic distribution of hematopoiesis at a clonal level is interest in understanding how hematopoietic stem and progenitor cells (HSPCs) their progeny interact with bone marrow (BM) niches during regeneration. We tagged rhesus macaque autologous HSPCs genetic barcodes, allowing tracking over time space after transplantation. found marked segregation CD34+ for least 6 mo posttransplantation, followed by very gradual mixing different BM sites subsequent months to years. Clonal mapping...
Introduction: Saliva represents a less invasive alternative to nasopharyngeal swab (NPS) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection. SalivaDirect is nucleic acid extraction-free method detecting SARS-CoV2 in saliva specimens. Studies evaluating the concordance of gold standard NPS and newly developed protocols are limited. The aim our study was assess as an COVID-19 testing. Methods: Matching samples were analysed from cohort symptomatic (n=127) asymptomatic...
Xenografts of the hematopoietic system are extremely useful as disease models and for translational research. Zebrafish xenografts have been widely used to monitor blood cancer cell dissemination homing due optical clarity embryos larvae, which allow unrestricted in vivo visualization migratory events. Here, we developed a xenotransplantation technique that transiently generates hundreds tissue chimeric by transplanting murine bone marrow cells into zebrafish blastulae. In contrast previous...
<ns4:p><ns4:bold>Introduction</ns4:bold>: Saliva represents a less invasive alternative to nasopharyngeal swab (NPS) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection. SalivaDirect is nucleic acid extraction-free method detecting SARS-CoV2 in saliva specimens. Studies evaluating the concordance of gold standard NPS and newly developed protocols are limited. The aim our study was assess as an COVID-19 testing.</ns4:p><ns4:p> <ns4:bold>Methods</ns4:bold>: Matching...
Surgical parabiosis of two animals different genetic backgrounds creates a unique scenario to study cell-intrinsic versus cell-extrinsic roles for candidate genes interest, migratory behaviors cells, and secreted signals in distinct settings. Because parabiotic share common circulation, any blood or blood-borne factor from one animal will be exchanged with its partner vice versa. Thus, cells molecular factors derived background can studied the context second background. Parabiosis adult mice...
It is well known that all numbers are normal of order k in base b also orders less than k. Another basic fact every real number if and only it simply for This may be interpreted to mean a blocks digits occur with the desired relative frequency along infinite arithmetic progression. We reinterpret these theorems Q-Cantor series expansions show they no longer true particularly strong way. The main theoretical result this paper will reduce problem constructing certain pathological properties...
Goal: The goal of this study was to evaluate the inpatient mortality risk among geriatric patients with inflammatory bowel disease (IBD). Background: challenges caring for elderly IBD will increase aging US population. Given complications hospitalization, we set examine if age older than 65 were at higher mortality. Materials and Methods: All ulcerative colitis (UC) or Crohn’s (CD) in National Inpatient Sample (NIS) from 2016 2017 as primary diagnosis secondary an IBD-related cause admission...
Ineffective esophageal motility (IEM) is the most common disorder identified on high-resolution manometry (HRM), but patients with this finding may be asymptomatic. Therefore, we aimed to identify specific HRM findings predictive of symptoms in IEM.Adult (≥18 y) who underwent between March 2016 and July 2019 were retrospectively evaluated reclassified according Chicago Classification 4.0 (CC4.0). Demographic information, parameters, gastroesophageal reflux disease Health-Related Quality Life...
To compare the effects of prophylactic indomethacin versus expectant management on short-term respiratory outcomes in extremely low-birth-weight (ELBW) infants.This was a retrospective cohort study ELBW infants with gestational age less than 28 weeks, born at level III neonatal intensive care unit from 2004 to 2009. Patients were grouped based whether they received or treatment. The key outcome cumulative number days mechanical ventilation. Other supplemental oxygen and continuous positive...
ABSTRACT Xenografts of the hematopoietic system are extremely useful as disease models and for translational research. Zebrafish xenografts have been widely used to monitor blood cancer cell dissemination homing due optical clarity embryos larvae, which allow unrestricted in vivo visualization migratory events. To broaden scope xenotransplantation studies zebrafish, we developed a technique that transiently generates tissue chimeras by transplanting murine bone marrow cells into zebrafish...