A5035120799- RNA modifications and cancer
- Acute Myeloid Leukemia Research
- Cancer-related molecular mechanisms research
- FOXO transcription factor regulation
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Hematopoietic Stem Cell Transplantation
- PARP inhibition in cancer therapy
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Chronic Myeloid Leukemia Treatments
- Genomics and Chromatin Dynamics
- Medical Imaging and Pathology Studies
- Autophagy in Disease and Therapy
- Obstructive Sleep Apnea Research
- Welding Techniques and Residual Stresses
- Cardiovascular Syncope and Autonomic Disorders
- Intraoperative Neuromonitoring and Anesthetic Effects
- Hemoglobinopathies and Related Disorders
- Pluripotent Stem Cells Research
- Cell death mechanisms and regulation
- Metal Forming Simulation Techniques
University of Florida
2018-2025
University of Florida Health
2020-2024
UF Health Cancer Center
2021
Peking Union Medical College Hospital
2021
Chinese Academy of Medical Sciences & Peking Union Medical College
2021
University of Illinois Chicago
2018-2020
University of Illinois Hospital & Health Sciences System
2018
Wuhan University
2014-2017
China National Offshore Oil Corporation (China)
2014
Abstract Faithful genome integrity maintenance plays an essential role in cell survival. Here, we identify the RNA demethylase ALKBH5 as a key regulator that protects cells from DNA damage and apoptosis during reactive oxygen species (ROS)-induced stress. We find ROS significantly induces global mRNA N6-methyladenosine (m6A) levels by modulating post-translational modifications (PTMs), leading to rapid efficient induction of thousands genes involved variety biological processes including...
Abstract FOXM1 , a known transcription factor, promotes cell proliferation in variety of cancer cells. Here we show that Foxm1 is required for survival, quiescence and self-renewal MLL-AF9 (MA9)-transformed leukemia stem cells (LSCs) vivo. Mechanistically, upregulation activates the Wnt/β-catenin signaling pathways by directly binding to β-catenin stabilizing protein through inhibiting its degradation, thereby preserving LSC quiescence, promoting MLL-rearranged AML. More importantly,...
Regulation of RNA substrate selectivity m6A demethylase ALKBH5 remains elusive. Here, we identify RNA-binding motif protein 33 (RBM33) as a previously unrecognized m6A-binding that plays critical role in ALKBH5-mediated mRNA demethylation subset transcripts by forming complex with ALKBH5. RBM33 recruits to its m6A-marked and activates activity through the removal SUMOylation. We further demonstrate is for tumorigenesis head-neck squamous cell carcinoma (HNSCC). promotes autophagy recruiting...
Constitutively active mutations of KRAS are prevalent in non-small cell lung cancer (NSCLC). However, the relationship between these and resistance to platinum-based chemotherapy underlying mechanisms remain elusive. In this study, we demonstrated that mutants confer platinum NSCLC. Mechanistically, mediate NSCLC cells by activating ERK/JNK signaling, which inhibits ALKBH5 m6A demethylase activity regulating post-translational modifications (PTMs) ALKBH5. Consequently, mutant leads a global...
Background and Purpose The poor prognosis of acute megakaryoblastic leukaemia (AMKL) means there is a need to develop novel therapeutic methods treat this condition. It was recently shown that inducing megakaryoblasts undergo terminal differentiation effective as treatment for AMKL. This encouraged us identify compound induces megakaryocyte differentiation, which could then act potent anti‐leukaemia agent. Experimental Approach effects tetrandrine on the expression CD41 cell morphology were...
MicroRNAs (miRNAs) are emerging as critical regulators of normal and malignant hematopoiesis. In previous studies acute myeloid leukemia miR-9 overexpression was commonly observed. Here, we show that ectopic expression in vitro vivo significantly blocks differentiation erythroid progenitor cells with an increase reactive oxygen species (ROS) production. Consistent this observation, ROS scavenging enzymes, including superoxide dismutase (Sod2), Catalase (Cat), glutathine peroxidase (Gpx1),...
The prognosis of acute megakaryoblastic leukemia (AMKL) is really dismal, which urges for development novel treatment. Baicalein one type flavonoids extracted from Scutellaria baicalensis Georgi (Huang Qin). It inhibited cell proliferation and subcutaneous tumor formation many lines. However, whether baicalein possesses anti-AMKL activities has not been tested.We found that potently multiple AMKL cells including CMK, CMY, Y10, 6133, 6133 MPL/W515L due to apoptosis cycle arrest at G1 phase....
Previously, we reported that ZNF300 might play a role in leukemogenesis. In this study, further investigated the function of K562 cells undergoing differentiation. We found upregulation coincided with megakaryocytic differentiation induced by phorbol-12-myristate-13-acetate (PMA) or erythrocytic cytosine arabinoside (Ara-C), respectively. To test whether promoted differentiation, knocked down and knockdown effectively abolished PMA-induced evidenced decreased CD61 expression. Furthermore,...
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Clonal hematopoiesis plays a critical role in the initiation and development of hematologic malignancies. In patients with del(5q) myelodysplastic syndrome (MDS), transcription factor FOXM1 is frequently downregulated CD34+ cells. this study, we demonstrated that Foxm1 haploinsufficiency disturbed normal conferred competitive repopulation advantage for short period. However, it impaired long-term self-renewal capacity hematopoietic stem cells, recapitulating phenotypes abnormal cells...
In the offshore oil and gas fields, facilities decommissioning research has become a very important topic. The should be deposit abandon after stop production. This paper proposes method of platform by studying this problem.
Abstract Stress granules (SGs) are crucial in RNA regulation, affecting cell fate and function. SGs contain RNAs, some of which can be methylated. We studied m 6 A modifications during the human CD34 + HSPCs (hCD34 ) differentiating into erythroid cells found that mRNAs encoding many erythroid-specific proteins had decreased methylation differentiation. Increased levels ALKBH5 demethylase erythropoiesis controls 3’UTR these mRNAs. hCD34 carrying mutations demonstrated a block erythropoiesis,...
Ecotropic viral integration site 1 (EVI1/MECOM) is frequently upregulated in myeloid malignancies. Here, we present an Evi1-transgenic mouse model with inducible expression hematopoietic stem/progenitor cells (HSPCs). Upon induction of Evi1 expression, mice displayed anemia, thrombocytopenia, lymphopenia, and erythroid megakaryocyte dysplasia a significant expansion committed progenitor cells, resembling human myelodysplastic syndrome/myeloproliferative neoplasm-like (MDS/MPN-like) disease....
Myeloproliferative neoplasms (MPN) are a group of blood cancers that boost normal cell production in the bone marrow. Abnormal mutations stem cells were found accompanying with occurrence MPN. It has been shown MPL (MPL W515L or W515K) involved patients Since tyrosine residues 625 and 630 mediate signaling, whether them affect W515L-induced myeloproliferative (MPNs) is unknown. In this study, we further tested their functions by substituting either both phenylalanine (termed as MPL515/625,...
Retrospective case series.This study aimed to determine the prevalence and risk factors for orthostatic hypotension (OH) in adolescents undergoing posterior spinal fusion deformity correction.The data of 282 consecutive who underwent correction our center over 12 months were retrieved. Patient characteristics, including whether laminectomy or osteotomy was performed during surgery, occurrence postoperative nausea vomiting (PONV), perioperative hemoglobin albumin changes, blood transfusion,...