A5078310248- Advanced Proteomics Techniques and Applications
- Cardiomyopathy and Myosin Studies
- Mass Spectrometry Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- CAR-T cell therapy research
- Metabolomics and Mass Spectrometry Studies
- Cardiovascular Effects of Exercise
- Cancer Immunotherapy and Biomarkers
- Cardiac Fibrosis and Remodeling
- Immunotherapy and Immune Responses
- Pluripotent Stem Cells Research
- Nutrition and Health in Aging
- Muscle metabolism and nutrition
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
- CRISPR and Genetic Engineering
- Electrochemical sensors and biosensors
- Calcium signaling and nucleotide metabolism
- Muscle Physiology and Disorders
- Peptidase Inhibition and Analysis
- Nicotinic Acetylcholine Receptors Study
- Biotin and Related Studies
- Signaling Pathways in Disease
Guangzhou Institutes of Biomedicine and Health
2017-2024
State Key Laboratory of Respiratory Disease
2017-2024
Chinese Academy of Sciences
2017-2024
Bridge University
2024
University of Calgary
2024
University of Wisconsin–Madison
2014-2023
Emory University
2023
Shanghai Ocean University
2021
Madison Group (United States)
2017
Indiana University – Purdue University Indianapolis
2015-2016
Top-down mass spectrometry (MS)-based proteomics is arguably a disruptive technology for the comprehensive analysis of all proteoforms arising from genetic variation, alternative splicing, and posttranslational modifications (PTMs). However, complexity top-down high-resolution spectra presents significant challenge data analysis. In contrast to well-developed software packages available in bottom-up proteomics, tools remain underdeveloped. Moreover, despite recent efforts develop algorithms...
Mass spectrometry (MS)-based top-down proteomics is a powerful method for the comprehensive analysis of proteoforms that arise from genetic variations and post-translational modifications (PTMs). However, MS high molecular weight (MW) proteins remains challenging mainly due to exponential decay signal-to-noise ratio with increasing MW. Size exclusion chromatography (SEC) favored size-based separation biomacromolecules but typically suffers low resolution. Herein, we developed serial size...
Heart failure (HF) is a leading cause of morbidity and mortality worldwide most often precipitated by myocardial infarction. However, the molecular changes driving cardiac dysfunction immediately after infarction remain poorly understood. Myofilament proteins, responsible for contraction relaxation, play critical roles in signal reception transduction HF. Post-translational modifications myofilament proteins afford mechanism beat-to-beat regulation function. Thus it paramount importance to...
Significance Hypertrophic cardiomyopathy (HCM) is a common genetic heart disease and leading cause of sudden cardiac death in young adults. HCM has been linked to mutations genes encoding sarcomeric proteins, but how different can result similar clinical phenotype unknown. Analysis surgical tissue samples from patients with severe outflow track obstruction using high-resolution mass spectrometry–based top-down proteomics revealed pattern altered proteoforms across tissues compared...
The rapid advancements in mass spectrometry (MS) instrumentation, particularly Fourier transform (FT) MS, have made the acquisition of high-resolution and high-accuracy measurements routine. However, software tools for interpretation MS data are underdeveloped. Although several algorithms automatic processing available, there is still an urgent need a user-friendly interface with functions that allow users to visualize validate computational output. Therefore, we developed MASH Suite,...
Human pluripotent stem cell (hPSC)-derived cardiomyocytes exhibit the properties of fetal cardiomyocytes, which limits their applications. Various methods have been used to promote maturation hPSC-cardiomyocytes; however, there is a lack an unbiased and comprehensive method for accurate assessment maturity hPSC-cardiomyocytes.
Analysis of protein phosphorylation remains a significant challenge due to the low abundance phosphoproteins and stoichiometry phosphorylation, which requires effective enrichment phosphoproteins. Here we have developed superparamagnetic nanoparticles (NPs) whose surface is functionalized by multivalent ligand molecules that specifically bind phosphate groups on any These NPs enrich from complex cell tissue lysates with high specificity as confirmed SDS-PAGE analysis phosphoprotein-specific...
Calcins are peptides from scorpion venom with the unique ability to cross cell membranes, gaining access intracellular targets. Ryanodine Receptors (RyR) ion channels that control release of Ca 2+ endoplasmic and sarcoplasmic reticulum. target RyRs induce long-lived subconductance states, whereby single-channel currents decreased. We used cryo–electron microscopy reveal binding structural effects imperacalcin, showing it opens channel pore causes large asymmetry throughout cytosolic assembly...
Sarcopenia, the loss of skeletal muscle mass and function with advancing age, is a significant cause disability independence in elderly thus, represents formidable challenge for aging population. Nevertheless, molecular mechanism(s) underlying sarcopenia-associated dysfunction remain poorly understood. In this study, we employed an integrated approach combining top-down targeted proteomics mechanical measurements to dissect age-related dysfunction. Top-down proteomic analysis uncovered...
Sarcopenia, the age-related loss of skeletal muscle mass and strength, is a significant cause morbidity in elderly major burden on health care systems. Unfortunately, underlying molecular mechanisms sarcopenia remain poorly understood. Herein, we utilized top-down proteomics to elucidate sarcopenia-related changes fast- slow-twitch muscles aging rats with focus sarcomeric proteome, which includes both myofilament Z-disc proteins-the proteins that constitute contractile apparatuses. Top-down...
Background In patients with hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), virus-specific cytotoxic T lymphocytes (CTLs) fail to eliminate HCC cells expressing HBV antigens. As the expression of viral antigen in HBV-associated may decrease allow tumor escape immune attacks, we hypothesized that an surface (HBsAg)-specific affinity-improved-T-cell receptor (TCR) will enable target more effectively than corresponding wild-type-TCR. We also postulated TCR promiscuity can be...
An integrated top-down phosphoproteomics strategy enabled by functionalized cobalt ferrite nanoparticle enrichment and online LC/MS/MS for identification, quantification, characterization of low abundance phosphoproteins is presented.
// Zhaoduan Liang 1 , Ye Tian Wenxuan Cai Zhiming Weng 3 Yanyan Li Huanling Zhang 1,2 Yifeng Bao and Yi 1,3 State Key Laboratory of Respiratory Disease, Guangzhou Institutes Biomedicine Health, Chinese Academy Sciences, Guangzhou, China 2 School Life University Science Technology China, Hefei, XiangXue Sciences Research Center, Pharmaceutical Co. Ltd., Correspondence to: Li, email: Keywords : high affinity, programmed cell death protein (PD-1), PD-ligand (PD-L1), soluble PD-L1, T cell,...
Myosin heavy chain (MHC), the major component of myosin motor molecule, plays an essential role in force production during muscle contraction. However, a comprehensive analysis MHC proteoforms arising from sequence variations and post-translational modifications (PTMs) remains challenging due to difficulties purifying (∼223 kDa) achieving complete coverage. Herein, we have established strategy effectively purify comprehensively characterize heart tissue by combining size-exclusion...
Determining changes in protein expression and post-translational modifications (PTMs) is crucial for elucidating cellular signal transduction disease mechanisms. Conventional antibody-based approaches have inherent problems such as the limited availability of high-quality antibodies batch-to-batch variation. Top-down mass spectrometry (MS)-based proteomics has emerged most powerful method characterization quantification modifications. Nevertheless, robust methods to simultaneously determine...
Abstract Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) exhibit a fetal phenotype that limits in vitro and therapeutic applications. Strategies to promote cardiomyocyte maturation have focused interventions on differentiated hPSC-CMs, but this study tests priming of early cardiac progenitor cells (CPCs) with polyinosinic-polycytidylic acid (pIC) accelerate maturation. CPCs were from hPSCs using monolayer differentiation protocol defined small molecule Wnt temporal modulation,...
The inhibitory checkpoint molecule programmed death (PD)-1 plays a vital role in maintaining immune homeostasis upon binding to its ligands, PD-L1 and PD-L2. Several recent studies have demonstrated that soluble PD-1 (sPD-1) can block the interaction between membrane enhance antitumor capability of T cells. However, affinity natural sPD-1 is too low permit therapeutic applications. Here, variant with approximately 3000-fold 70-fold increase bind PD-L2, respectively, was generated through...
Postinfarction remodeling and expansion of the peri-infarct border zone (BZ) directly correlate with mortality following myocardial infarction (MI); however, cellular molecular mechanisms underlying processes in BZ remain unclear. Herein, we utilized a label-free quantitative proteomics approach combination immunohistochemical analyses to gain better understanding contributing postinfarction swine model MI reperfusion. Our analysis uncovered significant down-regulation proteins involved...