A5088657051- Genetics, Aging, and Longevity in Model Organisms
- Algebraic Geometry and Number Theory
- Analytic Number Theory Research
- Parkinson's Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Endoplasmic Reticulum Stress and Disease
- Autism Spectrum Disorder Research
- Child Nutrition and Feeding Issues
- Family and Disability Support Research
- Circular RNAs in diseases
- Advanced Algebra and Geometry
- Adipose Tissue and Metabolism
- Cellular transport and secretion
- Congenital heart defects research
- RNA modifications and cancer
- Autophagy in Disease and Therapy
- Extracellular vesicles in disease
- Alzheimer's disease research and treatments
- RNA and protein synthesis mechanisms
- Nuclear Receptors and Signaling
- Cancer-related molecular mechanisms research
- Enzyme Catalysis and Immobilization
- Cryptography and Residue Arithmetic
- CRISPR and Genetic Engineering
Institute of Genetics and Developmental Biology
2018-2025
Chinese Academy of Sciences
2014-2025
University of Chinese Academy of Sciences
2006-2025
Harbin Medical University
2015-2025
University of Pennsylvania
2022-2025
Zhengzhou University
2025
Renmin Hospital of Wuhan University
2019-2024
Wuhan University
2019-2024
Xiamen University
2016-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
ABSTRACT Extracellular vesicles (EVs) have sparked tremendous interest owing to their prominent potential in diagnostics and therapeutics. Isolation of EVs from complex biological fluids with high purity is essential the accurate analysis EV cargo. Unfortunately, generally used isolation techniques do not offer good separation non‐EV contaminants. Hence, it important a standardized method characterise properties preparations, including size distribution, particle concentration, phenotype....
Extracellular vesicles (EVs) have stimulated considerable scientific and clinical interest, yet protein profiling sizing of individual EVs remains challenging due to their small particle size, low abundance proteins, overall heterogeneity. Building upon a laboratory-built high-sensitivity flow cytometer (HSFCM), we report here rapid approach for quantitative multiparameter analysis single down 40 nm with an rate up 10 000 particles per minute. Statistically robust size distribution was...
Vacuolar protein sorting-35 (VPS35) is essential for endosome-to-Golgi retrieval of membrane proteins. Mutations in the VPS35 gene have been identified patients with autosomal dominant PD. However, it remains poorly understood if and how deficiency or mutation contributes to PD pathogenesis. Here we provide evidence that links PD-like neuropathology. was expressed mouse dopamine (DA) neurons substantia nigra pars compacta (SNpc) STR (striatum)--regions are vulnerable. VPS35-deficient mice...
Highlights•Across species, poly-glutamine repeat proteins bind to and influence mitochondria•PolyQ other neuronal mitochondrial stress induce a cell-non-autonomous UPRmt•UPRmt pathway components are required for sensing PolyQ-induced stress•Serotonin synthesis secretion non-autonomous UPRmt inductionSummaryDuring neurodegenerative disease, the toxic accumulation of aggregates misfolded is often accompanied with widespread changes in peripheral metabolism, even cells which aggregating protein...
It has been demonstrated recently that extracellular vesicles (EVs) carry DNA; however, many fundamental features of DNA in EVs (EV-DNA) remain elusive. In this study, a laboratory-built nano-flow cytometer (nFCM) can detect single as small 40 nm diameter and fragments 200 bp upon SYTO 16 staining was used to study EV-DNA at the single-vesicle level. Through simultaneous side-scatter fluorescence (FL) detection particles with combination enzymatic treatment, present revealed that: (1) naked...
Abstract The aggregation and prion-like propagation of α-synuclein are involved in the pathogenesis Parkinson’s disease. However, underlying mechanisms regulating assembly spreading fibrils remain poorly understood. Tau co-deposits with brains disease patients, suggesting a pathological interplay between them. Here we show that tau interacts accelerates its aggregation. Compared pure fibrils, tau-modified enhanced seeding activity, inducing mitochondrial dysfunction, synaptic impairment...
Bloom syndrome is a rare autosomal disorder characterized by predisposition to cancer and genomic instability. BLM, the structural gene mutated in individuals with disorder, encodes DNA helicase belonging RecQ family of helicases. These helicases have been established serve roles both promoting preventing recombination. Mounting evidence has implicated function for BLM during replication; specifically, might be involved rescuing stalled or collapsed replication forks recombination-based...
N6 methylation at adenosine 1832 (m6A1832) of mammalian 18S rRNA, occupying a critical position within the decoding center, is modified by conserved methyltransferase, METTL5. Here, we find that METTL5 shows strong substrate preference toward A1832 motif but not other reported m6A motifs. Comparison with yeast ribosome structural model unmodified this site indicates modification may facilitate mRNA binding inducing conformation changes in ribosomal center. promotes p70-S6K activation and...
Mild mitochondrial stress experienced early in life can have beneficial effects on the span of organisms through epigenetic regulations. Here, we report that acetyl-coenzyme A (CoA) represents a critical signal to regulate aging chromatin remodeling and histone deacetylase complex (NuRD) Caenorhabditis elegans. Upon stress, impaired tricarboxylic acid cycle results decreased level citrate, which accounts for reduced production acetyl-CoA consequently induces nuclear accumulation NuRD...
We have been successful in our efforts to develop a long lived noncytolytic murine IL-10/Fc fusion protein. In the nonobese diabetic mouse (NOD) model, administration of from 5 25 wk age completely prevented occurrence diabetes. Moreover, these mice remained disease-free after cessation therapy. Immunohistochemistry studies show that treatment inhibits expression TNF-alpha, proinflammatory cytokine, as well Th1-type cytokines, IL-2 and IFN-gamma, but promotes IL-4 IL-10, Th2-type by...
Meta-omics approaches have been increasingly used to study the structure and function of microbial communities. A variety large-scale collaborative projects are being conducted encompass samples from diverse environments habitats. This change has resulted in enormous demands for long-term data maintenance capacity analysis. The Global Catalogue Metagenomics (gcMeta) is a part 'Chinese Academy Sciences Initiative Microbiome (CAS-CMI)', which focuses on studying human environmental microbiome,...
Abstract Viruses are by far the most abundant biological entities on our planet, yet existing characterization methods limited either their speed or lack of resolution. By applying a laboratory‐built high‐sensitivity flow cytometer (HSFCM) to precisely quantify extremely weak elastically scattered light from single viral particles, we herein report label‐free analysis viruses with resolution comparable that electron microscopy and throughput cytometry. The detection diameters down 27 nm is...
ABSTRACT Background The deposition of α‐synuclein (α‐Syn) in the brain is pathological hallmark Parkinson's disease (PD). Epidemiological data indicate that exposure to fine particulate matter (≤2.5 μm aerodynamic diameter [PM2.5]) associated with an increased risk for PD. Objective aim this study investigate whether PM2.5 has a direct effect on α‐Syn pathology and how it drives Methods was added into monomers different cell models test can promote fibrillization aggregation α‐Syn. A53T...