A5046514018- Microbial Metabolic Engineering and Bioproduction
- GABA and Rice Research
- Enzyme Structure and Function
- Probiotics and Fermented Foods
- Extracellular vesicles in disease
- Cancer Research and Treatments
- Bacterial Genetics and Biotechnology
- Photosynthetic Processes and Mechanisms
- Biochemical and Molecular Research
- Genomics and Phylogenetic Studies
- Biosensors and Analytical Detection
- Porphyrin Metabolism and Disorders
- CRISPR and Genetic Engineering
- Algal biology and biofuel production
- Viral Infections and Vectors
- RNA modifications and cancer
- Polyamine Metabolism and Applications
- Bacteriophages and microbial interactions
- Microbial Natural Products and Biosynthesis
- Protein purification and stability
- Lattice Boltzmann Simulation Studies
- Microfluidic and Bio-sensing Technologies
- Polysaccharides and Plant Cell Walls
- Microplastics and Plastic Pollution
- Escherichia coli research studies
Hunan University
2024
Shenzhen University
2024
State Key Laboratory of Chemobiosensing and Chemometrics
2024
Collaborative Innovation Center of Chemistry for Energy Materials
2020-2024
Xiamen University
2016-2024
National Cheng Kung University
2020-2024
Yueyang Changling Equipment Research Institute (China)
2024
Ministry of Natural Resources
2023
Second Institute of Oceanography
2023
It has been demonstrated recently that extracellular vesicles (EVs) carry DNA; however, many fundamental features of DNA in EVs (EV-DNA) remain elusive. In this study, a laboratory-built nano-flow cytometer (nFCM) can detect single as small 40 nm diameter and fragments 200 bp upon SYTO 16 staining was used to study EV-DNA at the single-vesicle level. Through simultaneous side-scatter fluorescence (FL) detection particles with combination enzymatic treatment, present revealed that: (1) naked...
Abstract Mitochondrial permeability transition (mPT)-mediated mitochondrial dysfunction plays a pivotal role in various human diseases. However, the intricate details of its mechanisms and sequence events remain elusive, primarily due to interference caused by Bax/Bak-induced outer membrane permeabilization (MOMP). To address these, we have developed methodology that utilizes nano-flow cytometry (nFCM) quantitatively analyze opening pore (mPTP), dissipation potential ( $$\Delta$$ <mml:math...
Correlated analysis of multiple biochemical parameters at the single-particle level and in a high-throughput manner is essential for insights into diversity functions biological nanoparticles (BNPs), such as bacteria subcellular organelles. To meet this challenge, we developed highly sensitive spectral nano-flow cytometer (S-nFCM) by integrating recording module to laboratory-built nFCM that 4–6 orders magnitude more side scattering detection 1–2 fluorescence than conventional flow...
The refractive index (RI) is a fundamental physical property of materials. Although measurement the RI biological nanoparticles (BNPs) in aqueous media great importance to basic research and biomedical applications, it hampered by their tiny size, large intrinsic heterogeneity, weak scattering. Here, we report development label-free technique that can determine individual viruses small extracellular vesicles (sEVs) with high precision an analysis rate up 10 000 particles per minute. This was...
Nasopharyngeal carcinoma (NPC) is a malignant tumor commonly associated with Epstein–Barr virus (EBV) infection, and its early diagnosis as well differentiation from nasopharyngitis (NPG) remains challenging due to the insufficient sensitivity of routine screening methods in clinical practice. To date, circulating extracellular vesicles (EVs, 40–1000 nm) have shown appealing potential liquid biopsy for cancer prognosis. Herein, nanoflow cytometry (nFCM) capable single EV analysis was applied...
5-Aminolevulinic acid (ALA) is an imperative compound that has been widely applied in the agricultural and medical fields. The ALA synthesis Escherichia coli via C4 pathway depends on synthase (ALAS) releases CO2 a single-step reaction. However, enhancement of production by increasing protein's quality quantity with simultaneous reduction never reported. In this study, expression activity ALAS were improved employing chaperone rare tRNAs. Moreover, pdxY gene was introduced for pyridoxal...
Protein aggregation is one of the greatest challenges in biopharmaceuticals as it could decrease therapeutic efficacy, induce immunogenicity, and reduce shelf life protein drugs. However, there lacks high-throughput methods than can count size aggregates nanometer range, especially for those smaller 100 nm. Employing a laboratory-built nano-flow cytometer (nFCM) that enables light scattering detection single silica nanoparticles small 24 nm with sizing resolution accuracy comparable to...
Blue carbon ecosystems have been widely recognized for their sequestration function to mitigate climate changes. However, both natural processes (e.g., interspecific competition) and human activities could alter the ability of blue storage. To clarify impacts vegetation succession reclamation on stock changes, field investigation laboratory analysis were conducted in a saltmarsh within Hangzhou Bay. Remote sensing data indicated zonation pattern: exotic species Spartina alterniflora...
Pyridoxal 5'-phosphate (pyridoxal phosphate, PLP) is an essential cofactor for multiple enzymatic reactions in industry. However, engineering based on PLP regeneration and related to the performance of enzymes chemical production has rarely been discussed. First, we found that MG1655 strain was sensitive nitrogen source relied different amino acids, thus biomass significantly reduced when excess medium. Then, six KEIO collection strains were applied find out prominent gene...