A5091694841- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Bacterial Genetics and Biotechnology
- Protein Hydrolysis and Bioactive Peptides
- Bacteriophages and microbial interactions
- Algal biology and biofuel production
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- RNA and protein synthesis mechanisms
- biodegradable polymer synthesis and properties
- Microbial Metabolites in Food Biotechnology
- Chemical and Physical Properties in Aqueous Solutions
- Biodiesel Production and Applications
- Organic Light-Emitting Diodes Research
- Cancer Research and Treatments
- Electrochemical sensors and biosensors
- Probiotics and Fermented Foods
- Fungal and yeast genetics research
- Studies on Chitinases and Chitosanases
Southern Taiwan University of Science and Technology
2016-2025
National Tsing Hua University
2021-2023
Tamkang University
2020-2022
National Chung Hsing University
2009-2016
National Taiwan University
2010-2016
National Taipei University of Technology
2016
Institute of Biological Chemistry, Academia Sinica
2016
National Taipei University
2016
National Central University
2015
National Changhua University of Education
2014
Microalgal strains of Scenedesmus obliquus have the great potential for production biofuels, CO2 fixation, and bioremediation. However, metabolic engineering S. to improve their useful phenotypes are still not fully developed. In this study, strain CPC2 was genetically engineered promote autotrophic growth lipid productivity. The overexpression plasmid containing type 2 diacylglycerol acyltransferse (DGAT) gene DGTT1 from Chlamydomonas reinhardtii constructed transformed into CPC2, positive...
Abstract Human milk oligosaccharides (HMOs) are multifunctional biomolecules crucial for infant health, with established roles in immune modulation, anti-inflammatory responses, antimicrobial defense, and neurodevelopment. However, their structural complexity limited natural availability necessitate the development of scalable synthetic methods to support advanced biological therapeutic investigations. This review highlights enzymatic synthesis microbial fermentation as key strategies...
Bacillus subtilis is most commonly employed for secretion of recombinant proteins. To circumvent the problems caused by using plasmids, T7 expression system known its high efficiency was rebuilt in B. subtilis. Accordingly, a markerless and replicon-free method developed genomic insertion DNAs. By act homologous recombination via guide DNA, suicidal vector carrying gene interest integrated into loci bacteria. Removal inserted selection marker replicon flanked FRT sites mediated FLP...
Abstract Genetic manipulation of cells for desired traits is the most appreciable strategy implemented in field bioengineering. However, this approach closely relies on use plasmids and commonly afflicted by potential problem plasmid instability safety caution. Meanwhile, it may also lead to spread antibiotic‐resistant markers with replicons environment. issue has long been neglected. In study, we have addressed these subjects developing replicon‐free markerless methods chromosomal insertion...
The emergence of antibiotic resistance has prompted scientists to search for new antibiotics. Transglycosylase (TGase) is an attractive target discovery due its location on the outer membrane bacteria and essential role in peptidoglycan synthesis. Though there have been a few molecules identified as TGase inhibitors past thirty years, none them developed into antibiotics humans. slow pace development perhaps lack continuous, quantitative, high-throughput assay available enzyme. Herein, we...
The pET system is commonly used for producing foreign proteins in Escherichia coli, but its reliance on the costly and metabolically demanding inducer IPTG limits industrial use. This study engineered a low-temperature inducible (LTIS) E. coli Nissle 1917 (EcN) by combining T7 expression with thermal mechanism CI857-λPRPL to generate new LTIS strain, ENL7P. strain ENL7P-sfGFP-Km underwent overnight culture at 37 °C 14–16 h, followed subculturing 30 24 h. resulted notable 5.53-fold increase...
Keratins, insoluble proteins with a robust structure, are major component of epidermal tissue and appendages such as hair, feathers, nails, walls. Keratinous waste mainly emanates from poultry leather industries, thereby severely contaminating the environment. Keratinase can lyse cross-linked structures, keratin, hence be used in animal feed, fertilizer, detergent, leather, pharmaceutical, cosmetic industries. Bacillus polyfermenticus B4, isolated feather compost, secretes keratinase to...
Nattokinase is a potent fibrinolytic enzyme with the potential for fighting cardiovascular diseases. Most recently, new Bacillus subtilis/Escherichia coli (B. subtilis/E. coli) shuttle vector has been developed to achieve stable production of recombinant nattokinase in B. subtilis (Chen; et al. 2007, 23, 808-813). With this strain, design an optimum but cost-effective medium high-level was attempted by using response surface methodology. On basis Plackett-Burman design, three critical...
Escherichia coli Nissle 1917 (EcN) is a probiotic used to treat gastrointestinal diseases. The and endotoxin-free characteristics of EcN support its potential be developed into microbial expression system. With this aim, in study, the powerful T7 system was constructed cryptic plasmid-free (EcNP) generate host ENL6P. concept multiple copies gene cassettes regulated by chromosomal promoter promoted due plasmid instability issues with protein production integration phage attachment sites...
Trehalose is a nonreducing disaccharide and has wide range of applications in food biorelated industry. This sugar can be synthesized from maltose one step by trehalose synthase. In this study, we attempted to overproduce synthase Picrophilus torridus (PTTS), thermoacidophilic archaea, Escherichia coli . However, overproduction PTTS was hampered when the T7 promoter-driven gene (PT7-PTTS) on multicopy plasmid employed E. The factors limiting production were identified systematic way,...