A5100318396- Microbial Natural Products and Biosynthesis
- Oral microbiology and periodontitis research
- Genomics and Phylogenetic Studies
- Dental materials and restorations
- Dental Implant Techniques and Outcomes
- Fungal Biology and Applications
- Salivary Gland Disorders and Functions
- Dental Health and Care Utilization
- Dental Radiography and Imaging
- Endodontics and Root Canal Treatments
- Heat shock proteins research
- Microbial Community Ecology and Physiology
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Bacteriophages and microbial interactions
- Bone Tissue Engineering Materials
- Microbial Metabolism and Applications
- Actinomycetales infections and treatment
- Dental Research and COVID-19
- Machine Learning in Bioinformatics
- Antifungal resistance and susceptibility
- Identification and Quantification in Food
- Genetic diversity and population structure
- Immune Response and Inflammation
- Dental Erosion and Treatment
Southwest University
2024-2025
University of Illinois Chicago
2019-2025
Shandong University of Traditional Chinese Medicine
2019-2025
Capital University
2024
Capital Medical University
2020-2024
University of Shanghai for Science and Technology
2023-2024
Beijing Tongren Hospital
2024
Subsurface Insights (United States)
2024
Jiangsu University
2024
Affiliated Hospital of Jiangsu University
2024
Abstract Objective This study sought to utilise machine learning methods in artificial intelligence select the most relevant variables classifying presence and absence of root caries evaluate model performance. Background Dental is one prevalent oral health problems. Artificial can be used develop models for identification risk gain valuable insights, but it has not been applied dentistry. Accurately identifying may guide treatment decisions, leading better outcomes. Methods Data were...
An actinomycete, strain FXJ46 T , was isolated from cypress forest soil in northern China and shown to have chemotaxonomic morphological properties consistent with streptomycetes. It developed greyish aerial mycelium pinkish-brown substrate on oatmeal agar. Phylogenetic analyses based an almost complete 16S rRNA gene sequence of the 120 nucleotide variable γ -region this molecule showed that it formed a distinct (but closely associated) line Streptomyces griseoaurantiacus DSM 40430 trees....
Pyrrolobenzodiazepines, a class of natural products produced by actinomycetes, are sequence selective DNA alkylating compounds with significant antitumor properties. Among the pyrrolo[1,4]benzodiazepines (PBDs) sibiromycin, one two identified glycosylated PBDs, displays highest affinity for and most potent Despite promising properties clinical trials sibiromycin were precluded cardiotoxicity effect in animals attributed to presence C-9 hydroxyl group. As first step toward development...
Regarding targeted disruption of epigenetic regulators, histone methyltransferase and deacetylase in a plant endophytic fungus Pestalotiopsis fici have been uncovered as an unexplored chemical repertoire. Manipulation regulators led to the isolation 15 new polyketides, including pestaloficiols T–W (1–3 5), well 11 macrodiolide ficiolides A–K (6–16). Ficiolide K (16) was found contain very rare 1,6-anhydro-pyranose moiety. Finally, biosynthetic origin characterized by isotope-labeling experiments.
Meta-omics approaches have been increasingly used to study the structure and function of microbial communities. A variety large-scale collaborative projects are being conducted encompass samples from diverse environments habitats. This change has resulted in enormous demands for long-term data maintenance capacity analysis. The Global Catalogue Metagenomics (gcMeta) is a part 'Chinese Academy Sciences Initiative Microbiome (CAS-CMI)', which focuses on studying human environmental microbiome,...
Abstract Background Host immunity plays an important role against oral microorganisms in periodontitis. Methods This study assessed the infiltrating immune cell subtypes 133 healthy periodontal and 210 chronic periodontitis tissues from Gene Expression Omnibus (GEO) datasets using CIBERSORT gene signature files. Results Plasma cells, naive B cells neutrophils were all elevated tissues, when compared to those controls. In contrast, memory resting dendritic, mast CD4 as well activated M1 M2...
Tomaymycin produced by Streptomyces achromogenes is a naturally pyrrolobenzodiazepine (PBD). The biosynthetic gene cluster for tomaymycin was identified and sequenced. analysis reveals novel pathway the anthranilate moiety of PBDs. Gene replacement chemical complementation studies were used to confirm proposed pathway.
Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal hematopoietic stem cell malignancies characterized by abnormal maturation, increased apoptosis bone marrow cells, and anemia. They the most common myeloid blood cancers in American adults. The full complement gene mutations that contribute to phenotypes or clinical symptoms MDS is not fully understood. Around 10%-25% patients harbor an interstitial heterozygous deletion on long arm chromosome 5 [del(5q)], creating...
The polarization of macrophages into an anti-inflammatory phenotype is crucial for resolving periodontal inflammation. It has been reported that B10 cells can regulate the immune response during inflammation and are also able to in periodontitis. However, whether cells' regulation function periodontitis related macrophage remains unclear. This study aims investigate
Abstract Lentinan (LNT) is a polysaccharide from Shiitake mushrooms that has significant effects regarding antioxidant, hypoglycemic, anti-inflammatory activities, etc., and this effect influenced by the human intestinal flora. However, interactions between LNT microorganisms, their metabolic processes, remain unclear. In study, fermentation characteristics of its on flora metabolites were studied in vitro fermentation. Our results indicated was effectively utilised microbiota, utilisation...
Abstract This study aims to investigate the role and mechanism of luteolin in inflammation phenotypic switch vascular smooth muscle cells (VSMCs) an arteriovenous fistula (AVF) model, for providing a potential agent prevention therapy AVF neointimal hyperplasia. In vivo, model is created Sprague Dawley rats. vitro, rat VSMCs are treated with platelet‐derived growth factor‐BB (PDGF‐BB) induce VSMCs. Histological changes analyzed using hematoxylin‐eosin. Western blot quantitative real‐time...
The search has been ongoing for safe and effective antimicrobial agents control prevention of oral biofilm associated with disease. Clinical trials specific anti-bacterials are costly often provide inconclusive results. simple approach ex vivo testing these not demonstrated utility, likely due to variability effects observed even a single donor. We show how shed biofilms, easily obtained from donor saliva, tested under optimized conditions, respond reproducibly anti-bacterial challenges...
Abstract Some species of Trichoderma are fungicolous on fungi and have been extensively studied commercialized as biocontrol agents. Multigene analyses coupled with morphology, resulted in the discovery T. hypoxylon sp. nov., which was isolated from surface stroma Hypoxylon anthochroum . The new taxon produces - to Verticillium -like conidiophores hyaline conidia. Phylogenetic based combined ITS, TEF1-α RPB2 sequence data indicated that is a well-distinguished strong bootstrap support...
A cyclodipeptide synthase-containing gene cluster (pcm) was identified in Streptomyces chrestomyceticus NA4264 through genome mining. Heterologous expression of the cryptic pcm albus led to production a new highly modified named purincyclamide (1). Bioinformatic analysis and knockout experiments revealed biosynthetic pathway. The 1 achieved one-pot enzymatic reaction.
Neonates with intrauterine growth retardation (IUGR) are susceptible to decreases in cellular immunity. In recent years, a growing body of evidence indicates that Hsp70 may serve as danger signal the innate immune system and promote receptor-mediated apoptosis. Using neonatal pigs IUGR, we investigated function expression heat shock protein 70 (Hsp70), nuclear factor-kappa B (NF-κB), forkhead box O 3a (FoxO3a) intestinal tract. Samples from blood, duodenum, jejunum, ileum normal weight (NBW)...
Up to date, little is known about the repair mode of microdamage in osteonal cortical bone resulting from screw implantation. In this study, self-tapping titanium screws were inserted into tibial diaphyses 24 adult male rabbits. The animals sacrificed at 1 day, 2 weeks, month and months after surgery. Histomorphometric measurement confocal microscopy performed on basic fuchsin stained sections examine morphological characteristics microdamage, resorption activity spatial relationship between...
A new benzamide (1) and four known compounds (2–5) were isolated from endophytic Streptomyces YIM67086, their structures determined as 2-amino-3,4-dihydroxy-5-methoxybenzamide (1), 4-hydroxy-3-methoxybenzoic acid (2), phenylacetic (3), N-acetyltyramine (4) p-hydroxytruxinic (5). Compound 5 was first found in the microorganism. The antimicrobial activities of 1–5 antioxidant activity compound 1 investigated.
Genome mining of the marine Streptomyces seoulensis A01 enabled identification a giant type I polyketide synthase gene cluster (asm). Heterologous expression cryptic asm using bacterial artificial chromosome vector in heterologous host led to production ansaseomycins A (1) and B (2). plausible biosynthetic pathway was also proposed. Additionally, compounds 1 2 are active against K562 cell lines with IC50 values 13.3 18.1 μM, respectively.
Endophytic fungi have proven to be prolific producers of bioactive secondary metabolites with agricultural applications. In this study, bioassay-guided isolation the endophytic fungus Acremonium vitellinum yielded four anthraquinone derivatives (compounds 1–4), including a previously undescribed dimethylated derivative bipolarin, 6,8-di-O-methylbipolarin (1). Their structures were determined by 1D and 2D nuclear magnetic resonance analysis as well high-resolution electrospray ionization mass...