A5100772092- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Plant Disease Resistance and Genetics
- Actinomycetales infections and treatment
- Plant Pathogens and Fungal Diseases
- RNA regulation and disease
- Microbial Community Ecology and Physiology
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Microbial Natural Products and Biosynthesis
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- interferon and immune responses
- Fungal Biology and Applications
- Mycobacterium research and diagnosis
- Viral Infections and Immunology Research
- Plant-Microbe Interactions and Immunity
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Liver physiology and pathology
- Yeasts and Rust Fungi Studies
- Molecular Biology Techniques and Applications
- PARP inhibition in cancer therapy
Peking University
2017-2025
Center for Life Sciences
2018-2025
Shenzhen Second People's Hospital
2021-2025
Harbin Institute of Technology
2023-2024
Shenzhen University Health Science Center
2024
Soochow University
2023-2024
Second Hospital of Tianjin Medical University
2024
Tianjin Medical University
2024
Liaocheng People's Hospital
2012-2023
State Key Laboratory of Protein and Plant Gene Research
2018-2023
The high mortality rate of immunocompromised patients with fungal infections and the limited availability highly efficacious safe agents demand development new antifungal therapeutics. To rapidly discover such agents, we developed a high-throughput synergy screening (HTSS) strategy for novel microbial natural products. Specifically, product library was screened hits that synergize effect low dosage ketoconazole (KTC) alone shows little detectable fungicidal activity. Through approximately...
Abstract Although long-read RNA-seq is increasingly applied to characterize full-length transcripts it can also enable detection of nucleotide variants, such as genetic mutations or RNA editing sites, which significantly under-explored. Here, we present an in-depth study detect and analyze sites in RNA-seq. Our new method, L-GIREMI, effectively handles sequencing errors read biases. Applied PacBio data, L-GIREMI affords a high accuracy identification. Additionally, our analysis uncovered...
An actinomycete, strain FXJ46 T , was isolated from cypress forest soil in northern China and shown to have chemotaxonomic morphological properties consistent with streptomycetes. It developed greyish aerial mycelium pinkish-brown substrate on oatmeal agar. Phylogenetic analyses based an almost complete 16S rRNA gene sequence of the 120 nucleotide variable γ -region this molecule showed that it formed a distinct (but closely associated) line Streptomyces griseoaurantiacus DSM 40430 trees....
We report a new method using re-designed guide RNAs with internal barcodes (iBARs) embedded in their loop regions. Our iBAR approach outperforms the conventional by producing screening results much lower false-positive and false-negative rates especially high multiplicity of infection (MOI). Importantly, reduces starting cells at MOI significantly greatly improved efficiency accuracy compared canonical CRISPR screens low MOI. This system is particularly useful when source limited or it...
Abstract Anti-PD-1/PD-L1 immune checkpoint blockade (ICB) therapy has revolutionized clinical cancer treatment, while abnormal PD-L1 or HLA-I expression in patients can significantly impact the therapeutic efficacy. Somatic mutations cells that modulate these critical regulators are closely associated with tumor progression and ICB response. However, a systematic interpretation of immune-related is still lacking. Here, we harnessed ABEmax system to establish large-scale sgRNA library...
A Gram-positive, rod-shaped, non-motile bacterium, designated strain wged11 T , was isolated from the root of ginseng, and its taxonomic position established using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that this organism formed robust clade with recognized species genus Leifsonia . Strain characterized by high content ω -cyclohexylundecanoic anteiso- iso-branched saturated fatty acids, MK-11 as major menaquinone dl -2,4-diaminobutyric acid in...
A high-G+C-content, Gram-positive bacterium, strain D10 T , was isolated from the root of Oroxylum indicum a Chinese medicinal plant. Based on 16S rRNA gene sequence analysis, member genus Pseudonocardia and most closely related, albeit loosely, to halophobica . Morphological chemotaxonomic characteristics support affiliation Results DNA–DNA hybridization physiological biochemical tests allowed genotypic phenotypic differentiation related species. Strain (=CGMCC 4.3143 =DSM 44984 ) therefore...
The taxonomic status of six neutrotolerant acidophilic streptomycetes isolated from acidic soils in Yunnan Province, China, was established using a polyphasic approach. morphological and chemotaxonomic characteristics revealed that the isolates belong to genus Streptomyces. Almost complete 16S rRNA gene sequences were determined aligned with available corresponding representatives family Streptomycetaceae; phylogenetic trees inferred four tree-making algorithms. formed distinct, albeit...
The taxonomic positions of two gamma- and UV-ray-resistant strains isolated from radiation-polluted soil in north-west China were determined a polyphasic study. organisms, designated R12 T R13 , Gram-stain-positive, non-spore-forming cocci, which contained MK-8 as the major respiratory quinone C 16 : 1 ω 7 c 0 fatty acids. cell walls ornithine. Phylogenetic analysis based on 16S rRNA gene sequences DNA–DNA hybridizations showed that are members novel species belonging to genus Deinococcus,...
A thermotolerant, Gram-stain-positive, aerobic, sporangium-forming actinomycete, strain RA45(T), was isolated from a desert region in Xinjiang Uigur Autonomous Region, north-western China. Comparative analysis of the 16S rRNA gene sequence and phenotypic characterization revealed that RA45(T) belonged phylogenetically to family Pseudonocardiaceae suborder Pseudonocardineae. Strain showed more than 5 % divergence recognized species genera Pseudonocardiaceae, forming distinct lineage within...
Abstract Background Breast cancer is the most common and leading cause of mortality in women worldwide. Hypoxia an important factor involved progression solid tumors has been associated with various indicators tumor metabolism, angiogenesis metastasis. But little known about contribution Hypoxia-Inducible Factor-2a (HIF-2a) to drug resistance clinicopathological characteristics breast cancer. Methods Immunohistochemistry was employed on tissue microarray paraffin sections surgically removed...
The strawberry is an important fruit worldwide. development of the industry limited by fungal disease. Anthracnose caused pathogen Colletotrichum gloeosporioides and leads to large-scale losses in terms quality production. Different cultivars have different response this pathogen. However, genetic mechanisms underlying difference transcriptional infection with C. poorly understood. In present study, leaf transcriptome 'Yanli' 'Benihoppe' were deep sequenced via RNA-seq analysis study...
Background Thiopental is frequently used for the treatment of intracranial hypertension after severe head injury. Its long-term administration increases incidence nosocomial infections, which contributes to high mortality rate these patients. However, mechanism its immunosuppressing effect remains unknown. Methods The thiopental (200-1000 microg/ml) on activation nuclear transcription factor kappaB (NF-kappaB; electrophoretic mobility shift assays), NF-kappaB-driven reporter gene activity...