A5032684810- SARS-CoV-2 and COVID-19 Research
- Neuroscience and Neuropharmacology Research
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Neurotransmitter Receptor Influence on Behavior
- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Ion channel regulation and function
- SARS-CoV-2 detection and testing
- Protein Structure and Dynamics
- Cardiac, Anesthesia and Surgical Outcomes
- Parkinson's Disease Mechanisms and Treatments
- Vaccine Coverage and Hesitancy
- Anesthesia and Pain Management
- Animal Virus Infections Studies
- Genomics and Phylogenetic Studies
- Animal Disease Management and Epidemiology
- Enhanced Recovery After Surgery
- COVID-19 Clinical Research Studies
- RNA Interference and Gene Delivery
- Protein purification and stability
- Protist diversity and phylogeny
- Extracellular vesicles in disease
Fudan University
2024
Harbin Veterinary Research Institute
2020-2023
Chinese Academy of Agricultural Sciences
2020-2023
Wuhan University
2023
Peking University
2019-2022
Center for Life Sciences
2019-2022
State Key Laboratory of Protein and Plant Gene Research
2019-2022
Air Force Medical University
2013-2022
Chiba University
2017-2022
People's Liberation Army 401 Hospital
2021
As the emerging variants of SARS-CoV-2 continue to drive worldwide pandemic, there is a constant demand for vaccines that offer more effective and broad-spectrum protection. Here, we report circular RNA (circRNA) vaccine elicited potent neutralizing antibodies T cell responses by expressing trimeric RBD spike protein, providing robust protection against in both mice rhesus macaques. Notably, circRNA enabled higher durable antigen production than 1mΨ-modified mRNA proportion distinct...
Journal Article Improved methods for structure probing in large RNAs: a rapid 'heterologous' sequencing approach is coupled to the direct mapping of nudease accessible sites. Application 5′nal domain eukaryotic 28S rRNA Get access Liang Hu Qu, Qu Centre de Recherches Biochimie et Génétique Cellulaires du CNRS118, route Narbonne, 31062 Toulouse Cédex, France Search other works by this author on: Oxford Academic PubMed Google Scholar Bernard Michot, Michot Jean-Pierre Bachellerie * *To whom...
Significance African swine fever virus (ASFV) causes a severe and highly contagious disease in pigs wild boars, but no commercial vaccines or antivirals are available currently. Understanding the mutual antagonism between host factors during ASFV infection may facilitate development of new antivirals. Our work profiled transcriptomes macrophages infected with through single-cell RNA-sequencing technology. Identified dynamic transcriptome events viral genes provide molecular characteristics...
Using a rapid rRNA sequencing technique, we have determined the sequence of 400 nucleotides located at 5' end large subunit molecule from eight species unicellular eukaryotes (protists). This region contains pair conservative domains well-suited for long-range phylogenetic evaluations among eukaryotes, due both to their substantia, length and intrinsic rate variation during evolution. It also comprises central more rapidly evolving portion, which allows fine tuning distance evaluation...
Abstract NVSI-06-08 is a potential broad-spectrum recombinant COVID-19 vaccine that integrates the antigens from multiple SARS-CoV-2 strains into single immunogen. Here, we evaluate safety and immunogenicity of as heterologous booster dose in BBIBP-CorV recipients randomized, double-blind, controlled, phase 2 trial conducted United Arab Emirates (NCT05069129). Three groups healthy adults over 18 years age (600 participants per group) who have administered two doses 4-6-month, 7-9-month...
The increased coronavirus disease 2019 (COVID-19) breakthrough cases pose the need of booster vaccination. We conducted a randomised, double-blinded, controlled, phase 2 trial to assess immunogenicity and safety heterologous prime-boost vaccination with an inactivated COVID-19 vaccine (BBIBP-CorV) followed by recombinant protein-based (NVSI-06-07), using homologous boost BBIBP-CorV as control. Three groups healthy adults (600 individuals per group) who had completed two-dose vaccinations 1-3...
Abstract Chimeric Antigen Receptor (CAR) T cell therapy has shown promise in treating hematologic malignancies. However, it is limited to individualized and faces challenges, including high costs, extended preparation time, efficacy against solid tumors. Here, we generated circular RNAs (circRNAs) encoding transmembrane proteins, referred as circRNA CAR , which mediated remarkable tumor killing both cells macrophages. In addition, macrophages exhibited efficient phagocytosis of...
Objective: To prospectively evaluate the efficacy of a neurosurgical enhanced recovery after surgery (ERAS) protocol on management postoperative pain elective craniotomies.Methods: This randomized controlled trial was conducted in center Tangdu Hospital (Fourth Military Medical University, Xi'an, China).A total 129 patients undergoing craniotomies between October 2016 and July 2017 were enrolled clinical comparing an ERAS to conventional care regimen.The primary outcome score assessed by...
Efficient and eco-friendly disinfection of air-borne human respiratory RNA viruses is pursued in both public environment portable usage. The AlGaN-based deep ultraviolet (DUV) light-emission diode (LED) has high practical potentials because its advantages variable wavelength, rapid sterilization, environmental protection, miniaturization. Therefore, whether the emission wavelength effects on as well device feasible to sterilize various under conditions crucial. Here, we fabricate DUV LEDs...
Abstract How does SARS-CoV-2 cause lung microenvironment disturbance and inflammatory storm is still obscure. We here performed the single-cell transcriptome sequencing from lung, blood, bone marrow of two dead COVID-19 patients detected cellular communication among them. Our results demonstrated that infection increase frequency between alveolar type I cells (AT1) or II (AT2) myeloid triggering immune activation inflammation then induce disorder fibroblasts, club, ciliated cells, which may...
Antibodies are one of the most important protein molecules in biopharmaceutics. Due to recent advance technology for producing monoclonal antibodies, many structural data available on antigen–antibody complexes. To characterize molecular interaction recognition, we computationally analyzed 500 complex structures by mechanics calculations. The presence Ser and Tyr is markedly large complementarity-determining regions (CDRs). Although abundant CDRs, its contribution binding score not large....
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its emerging variants of concern (VOC), such as Delta (B.1.617.2) Omicron (B.1.1.529), has continued to drive the worldwide pandemic. Therefore, there is a high demand for vaccines with enhanced efficacy, thermostability, superior design flexibility, fast manufacturing speed. Here, we report circular RNA (circRNA) vaccine that encodes trimeric RBD SARS-CoV-2 Spike protein. Without need nucleotide modification,...
Classical swine fever virus (CSFV) is a member of the genus Pestivirus in Flaviviridae family. To date, host factors required for CSFV entry remain poorly characterized. identify functional membrane protein(s) involved infection, we analyzed transcriptomic data from previous studies describing gene expression profiles CSFV, and found twelve novel candidate proteins. One these proteins, MERTK, significantly reduced protein by RNA interference screening using recombinant that contains...
Accurate estimation of ligand-receptor binding affinity is indispensable for computer-assisted drug discovery and structure-based design. Many computational scoring functions estimating have been proposed. Every function reported so far, however, has strengths weaknesses depending on the chemical properties ligands feature site receptor. Hence, potential that can be used many kinds target proteins are required. In this work, we developed a software program based Morse-type enables evaluation...
Abstract Endoscopic endonasal transsphenoidal resection has been accepted as a routine therapy for pituitary adenoma, but the postoperative hospital stay is typically several days long. With advantages of reduced cost and improved patient satisfaction, application ambulatory surgery (AS) developed rapidly. However, AS was still rarely adopted in neurosurgery. Here we designed an treatment protocol adenoma with endoscopic approach (EEA), reported our initial experiences regarding safety...
Antibodies are crucial biomolecules that bring high therapeutic efficacy in medicine and accurate molecular detection diagnosis. Many studies have been devoted to analyzing the antigen–antibody interaction from importance of understanding antibody recognition mechanism. However, most previous examined characteristic for interaction. It is also informative clarify significant antigen residues contributing binding. To characterize antigens, we computationally analyzed 350 complex structures by...
Rationale: Neuroadaptations in the medial prefrontal cortex (mPFC) and Nucleus Accumbens (NAc) play a role disruption of control-reward circuits opioid addiction.Small Conductance Calcium-Activated Potassium (SK) channels mPFC have been implicated neuronal excitability changes during morphine withdrawal.However, mechanism that modulates SK withdrawal is still unknown.Methods: Rats were exposed for one week to daily injections (10 mg•kg -1 s.c.) followed by conditional place preference (CPP)...