A5100699622- HER2/EGFR in Cancer Research
- Monoclonal and Polyclonal Antibodies Research
- Metabolism, Diabetes, and Cancer
- Estrogen and related hormone effects
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Histone Deacetylase Inhibitors Research
- Enhanced Recovery After Surgery
- PI3K/AKT/mTOR signaling in cancer
- Glioma Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Growth Hormone and Insulin-like Growth Factors
- Cardiac, Anesthesia and Surgical Outcomes
- Cell death mechanisms and regulation
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Protein Degradation and Inhibitors
- Glycosylation and Glycoproteins Research
- Anesthesia and Pain Management
- Cancer-related molecular mechanisms research
- Brain Metastases and Treatment
- MicroRNA in disease regulation
- Cancer, Lipids, and Metabolism
- Cancer-related gene regulation
Louisiana State University Health Sciences Center New Orleans
2019-2025
Institute of Food Science and Technology
2024-2025
Chinese Academy of Agricultural Sciences
2024-2025
Hefei University of Technology
2016-2025
Stanley Foundation
2025
Anhui Provincial Center for Disease Control and Prevention
2020-2024
Lanzhou Jiaotong University
2024
Zhengzhou University
2024
Beijing Institute of Technology
2021-2024
Peking University Cancer Hospital
2024
The anti-diabetic drug metformin reduces human cancer incidence and improves the survival of patients, including those with breast cancer. We studied activity against diverse molecular subtypes cell lines in vitro. Metformin showed biological all estrogen receptor (ER) positive negative, erbB2 normal abnormal tested. It inhibited cellular proliferation, reduced colony formation caused partial cycle arrest at G1 checkpoint. did not induce apoptosis (as measured by DNA fragmentation PARP...
AbstractTriple negative (TN) breast cancer is more frequent in women who are obese or have type II diabetes, as well young Women of Color. These cancers do not express receptors for the steroid hormones estrogen progesterone, receptor tyrosine kinase (RTK) Her-2 but upregulation basal cytokeratins and epidermal growth factor (EGFR). data suggest that aberrations glucose fatty acid metabolism, signaling through EGFR genetic factors may promote development TN cancers. The anti-type diabetes...
Trastuzumab shows remarkable efficacy in treatment of ErbB2-positive breast cancers when used alone or combination with other chemotherapeutics. However, acquired resistance develops most treated patients, necessitating alternate strategies. Increased aerobic glycolysis is a hallmark cancer and inhibition may offer promising strategy to preferentially kill cells. In this study, we investigated the antitumor effects trastuzumab inhibitors cancer. We found that inhibits via downregulation heat...
Non-small cell lung cancer (NSCLC) is the leading cause of death, reflecting need for better understanding oncogenesis, and developing new diagnostic therapeutic targets malignancy. Emerging evidence suggests that small nucleolar RNAs (snoRNAs) have malfunctioning roles in tumorigenesis. Our recent study demonstrated RNA 42 (SNORA42) was overexpressed tumors. Here, we investigate role SNORA42 tumorigenesis NSCLC. We simultaneously assess genomic dosages expression levels its host gene,...
Abstract Primary and acquired resistance to the breast cancer drug trastuzumab (Herceptin) is a significant clinical problem. Here, we report enhanced activation of downstream signaling pathways emanating from growth factor receptors erbB2, erbB3, insulin-like factor-I receptor (IGF-IR) in trastuzumab-resistant cells. Interactions between IGF-IR erbB2 or erbB3 occur exclusively cells, where erbB2-erbB3 interactions are also observed. Moreover, these three form heterotrimeric complex...
Abstract Clioquinol, a metal chelator, has been used for many years as an antimicrobial agent and more recently potential treatment Alzheimer's disease. Because it binds copper zinc, metals essential the activity of enzyme superoxide dismutase-1 (SOD1), target anticancer drug development, we investigated its effects on human cancer cells. Treatment with clioquinol reduced viability eight different cell lines in concentration-dependent manner, IC50 values low micromolar range. Biochemical...
A distinct group of breast cancers, called "basal" or "triple-negative" (TN) cancers express both basal cytokeratins and the epidermal growth factor receptor, but fail to estrogen receptors, progesterone receptors HER2 have stem-like mesenchymal features. They are particularly aggressive, frequently chemo-resistant, with p53 mutation, up-regulation IL-6 Stat3. Because TN cells sensitive anti-diabetic agent metformin, we hypothesized that it may target JAK2/Stat3 signaling. The effects...
Perlecan, a multidomain heparan sulfate proteoglycan (PG), is an intrinsic component of basement membranes and extracellular matrices. We used prokaryotic expression vector to generate fusion proteins encoding various domains human perlecan protein core these recombinant were as immunogens produce mouse anti-human monoclonal antibodies (MAb). One MAb, designated 7B5, was characterized by Western blotting ELISA shown react specifically with the laminin-like region (Domain III) but not two...
Significance We describe the smallest (220 nt) replicating covalently closed circular (CCC) RNA (nanogenome) that contains numerous unique and novel properties of coding translation. It is all viroids virusoids only one to encode proteins. No noncoding sequences could be identified. Initiation termination codons are combined in UGAUGA, read-throughs generate larger This CCC directly translated by eukaryotic ribosomes. also an internal ribosome entry site it defies classical Kozak scanning...
Single-hole hollow polymer microspheres (see Figure) have been synthesized through the direct polymerization and cross-linking reactions occurring at surface of carboxyl-capping polystyrene colloids, followed by removal core particles. The molecular imprinting these single-hole towards specific high-capacity uptake target species opens new routes to promising applications.
Abstract Receptor tyrosine kinase activity is essential for erbB2 (HER2/ neu ) promotion of breast carcinogenesis, metastasis and therapeutic resistance. can be activated by dimerization with another erbB receptor, most which bind ligands. Of these, the erbB2/erbB3 heterodimer potent oncogenic complex. reportedly requires erbB3 to promote cellular proliferation, although this may occur without changes in some model systems. Our investigations focus on role(s) erbB2‐associated tamoxifen Using...
AbstractWe have shown that erbB2 altered breast cancer cells are less sensitive to the anti-proliferative effects of metformin than triple negative cells, and described differences molecular mechanisms action by tumor subtypes. We hypothesized may be more effective against trastuzumab-resistant erbB2-overexpressing because it targets critical signaling pathways with resistance. BT474, SKBR3 derived sublines BT474-HR20 (HR20) SKBR3-pool2 (pool2) were used test this hypothesis. Metformin...
Metformin treatment has been associated with a decrease in breast cancer risk and improved survival. induces complex cellular changes, resulting decreased tumor cell proliferation, reduction of stem cells, apoptosis. Using carcinogen-induced rodent model mammary tumorigenesis, we recently demonstrated that overfeeding obese animals is 50% increase glucose uptake, increased reprogramming to an "aggressive" metabolic state. significantly inhibited these pro-tumorigenic effects. We hypothesized...
We reported that the class I HDAC inhibitor entinostat induced apoptosis in erbB2-overexpressing breast cancer cells via downregulation of erbB2 and erbB3. Here, we study molecular mechanism by which dual-targets erbB2/erbB3. Treatment with had no effect on erbB2/erbB3 mRNA, suggesting a transcription-independent mechanism. Entinostat decreased endogenous but not exogenous erbB2/erbB3, indicating it did alter their protein stability. hypothesized might inhibit translation specific miRNAs....
Abstract Long noncoding RNA (lncRNA) plays pivotal roles in cancer development. To date, only a small number of lncRNAs have been characterized at functional level. Here, we discovered novel lncRNA termed GAS5-AS1 as tumor suppressor non-small cell lung (NSCLC). The expression NSCLC tumors was much lower than that the adjacent normal tissues. reduced significantly correlated with larger tumors, higher TNM stages and lymph node metastasis patients. While ectopic or specific knockdown had no...
OBJECTIVE Although enhanced recovery after surgery (ERAS) programs have gained acceptance in various surgical specialties, no established neurosurgical ERAS protocol for patients undergoing elective craniotomy has been reported the literature. Here, authors describe design, implementation, safety, and efficacy of a novel tertiary care medical center located China. METHODS A multidisciplinary was developed based on best available evidence. total 140 between October 2016 May 2017 were enrolled...
Abstract Resistance to Herceptin represents a significant challenge for successful treatment of HER2-positive breast cancer. Here, we show that in Herceptin-sensitive cells, FOXO3a regulates specific miRNAs control IGF2 and IRS1 expression, retaining basic IGF2/IGF-1R/IRS1 signaling. The activity maintains expression PPP3CB, subunit the serine/threonine-protein phosphatase 2B, restrict phosphorylation (p-FOXO3a), inducing - -targeting miRNAs. However, Herceptin-resistant p-FOXO3a levels are...
Abstract Resistance to HER2-targeted therapy represents a significant challenge for the successful treatment of patients with breast cancer HER2-positive tumors. Through global mass spectrometry–based proteomics approach, we discovered that expression N6-methyladenosine (m6A) demethylase ALKBH5 was significantly upregulated in therapy-resistant cells. Elevated sufficient confer resistance therapy, and specific knockdown rescued efficacy trastuzumab lapatinib resistant Mechanistically,...
Wild-type erbB-2/neu transgenic mice were used to study the interactions between tamoxifen and dietary phytoestrogens (or isoflavones) by dose form in vivo. Mice randomized one of four formulas implanted with an 8-week continuous-release or placebo pellet at 8 weeks age. In placebo-treated mice, soy meal diet (but not diets supplemented low-dose high-dose isoflavones a casein diet) resulted prolongation tumor latency. tamoxifen-treated fed meal, casein, isoflavone enriched diets, majority...