A5004715887- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Streptococcal Infections and Treatments
- Chronic Lymphocytic Leukemia Research
- Immune cells in cancer
- Immunotherapy and Immune Responses
- RNA regulation and disease
- Acute Lymphoblastic Leukemia research
- Cancer Immunotherapy and Biomarkers
- Neurobiology and Insect Physiology Research
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Glioma Diagnosis and Treatment
- Invertebrate Immune Response Mechanisms
- Antimicrobial Resistance in Staphylococcus
- Cancer Genomics and Diagnostics
- Animal Behavior and Reproduction
- Histone Deacetylase Inhibitors Research
- Hematopoietic Stem Cell Transplantation
- Bacterial biofilms and quorum sensing
- thermodynamics and calorimetric analyses
- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- Biosimilars and Bioanalytical Methods
- Ubiquitin and proteasome pathways
Oregon Health & Science University
2017-2025
University of California, Los Angeles
2020-2024
University of Southern California
2024
Methodist Hospital
2023-2024
Houston Methodist
2023-2024
University of Portland
2024
Stanford University
2019
Family Research Institute
2015
Abstract Although previous studies have investigated the role of IL-27/WSX-1 interactions in regulation Th1 responses, little is known about their regulating Th2-type responses. Studies presented this work identify a direct for negative type 2 responses independent effects on 1 cytokines. WSX-1−/− mice infected with gastrointestinal helminth Trichuris muris displayed accelerated expulsion parasites and development exaggerated goblet cell hyperplasia mastocytosis gut due to increased...
Abstract Although long-read RNA-seq is increasingly applied to characterize full-length transcripts it can also enable detection of nucleotide variants, such as genetic mutations or RNA editing sites, which significantly under-explored. Here, we present an in-depth study detect and analyze sites in RNA-seq. Our new method, L-GIREMI, effectively handles sequencing errors read biases. Applied PacBio data, L-GIREMI affords a high accuracy identification. Additionally, our analysis uncovered...
Dedifferentiation programs are commonly enacted during breast cancer progression to enhance tumor cell fitness. Increased cellular plasticity within the neoplastic compartment of tumors correlates with disease aggressiveness, often culminating in greater resistance cytotoxic therapies or augmented ability metastasize distant organs. Here we report that subpopulations dedifferentiated epithelial cells express canonical leukocyte surface receptor proteins and have thus named this program...
Abstract Dedifferentiation programs are commonly enacted during breast cancer progression to enable novel cellular phenotypes. Increased plasticity within the neoplastic compartment of tumors correlates with disease aggressiveness, often culminating in greater resistance cytotoxic therapies or augmented ability metastasize distant organs. Here we report that subpopulations dedifferentiated epithelial cells express canonical leukocyte cell surface receptor proteins and have named this unique...
Abstract The human pathogen Streptococcus pyogenes secretes a short peptide (leaderless communication peptide, LCP) that mediates intercellular and controls bacterial virulence through interaction with its receptor, RopB. Here, we show LCP RopB homologues are present in other Firmicutes. We experimentally validate LCPs distinct codes act as signals regulate gene expression salivarius , porcinus Enterococcus malodoratus Limosilactobacillus reuteri . Our results indicate more widespread than...
The squamates (lizards and snakes) are close relatives of birds mammals, with more than 10,000 described species that display extensive variation in a number important biological traits, including coloration, venom production, regeneration. Due to lack genomic tools, few genetic studies have been carried out. leopard gecko, Eublepharis macularius , is popular companion animal, displays variety coloration patterns. We took advantage large breeding colony used linkage analysis, synteny,...
Abstract Allo CAR T cells represent an “off-the-shelf” treatment option that addresses the unmet need for immediately available, potentially curative therapies. CRISPR Therapeutics’ first generation allo cell products, CTX110® (CD19-directed) and CTX130TM (CD70-directed), have shown encouraging efficacy in ongoing clinical trials (NCT04035434, NCT04502446, NCT04438083), including complete durable responses B- T-cell lymphoma renal carcinoma (RCC). However, exhaustion, occurring even prior to...
In complex, highly unstable genomes such as in osteosarcoma, targeting aberrant checkpoint processes (metabolic, cell cycle or immune) may prove more successful than specific kinase growth factor signaling pathways. Here, we establish a comparative oncology approach characterizing the most lethal osteosarcomas identified biorepository of tumors from three different species: human, mouse and canine. We describe development genetically-engineered model establishment primary cultures fatal...
Abstract The squamates (lizards and snakes) are close relatives of birds mammals, with more than 10,000 described species that display extensive variation in a number important biological traits, including coloration, venom production, regeneration. Due to lack genomic tools, few genetic studies have been carried out. leopard gecko 1 , Eublepharis macularius, is popular companion animal, displays variety coloration patterns. We took advantage large breeding colony used linkage analysis,...
Abstract Dedifferentiation programs are commonly enacted during breast cancer progression where they enable novel cellular phenotypes. Increased plasticity within the neoplastic compartment of tumors correlates with disease aggressiveness, often manifesting as greater resistance to cytotoxic therapies or increased ability metastasize distant organs. Here we report that subpopulations ostensibly stem-like epithelial cells express canonical leukocyte cell surface receptor proteins, and have...
The interplay between host nutritional immune mechanisms and bacterial nutrient uptake systems has a major impact on the disease outcome. factor calprotectin (CP) limits availability of essential transition metals, such as manganese (Mn) zinc (Zn), to control growth invading pathogens. We previously demonstrated that competition CP human pathogen group A streptococcus (GAS) for Zn impacts GAS pathogenesis. However, contribution Mn sequestration by in infection role acquisition overcoming...
ABSTRACT Cancer patients with advanced disease exhaust available clinical regimens and lack actionable genomic medicine results, leaving a large patient population without effective treatments options when their inevitably progresses. To address the unmet need for evidence-based therapy assignment standard approaches have failed, we developed probabilistic computational modeling approach which integrates sequencing data functional assay to develop patient-specific combination cancer...
Abstract The gram-positive bacteria use quorum sensing secreted peptides to communicate and control critical bacterial phenotypes. All the are produced in a longer inactive form that is processed into shorter active peptides. Recently, we discovered new class of peptide human pathogen Streptococcus pyogenes , leaderless communication (LCP), encoded as an 8 amino acid long without secretion signal sequence processing sites. Nevertheless, LCP mediates intercellular controls virulence. However,...
<h3>Background</h3> CTX110, a CD19-directed first-generation CRISPR-edited CAR T cell therapeutic candidate for B-cell lymphoma, has induced clinical responses and durable remissions (2+ years) in some patients (CARBON trial, NCT04035434). CTX112 contains the same edits as CTX110 to disrupt B2M locus immune evasion TRAC prevent GvHD allow transgene integration. Additionally, disruptions TGFBR2 gene avoid suppression of activity by cells tumor environment Regnase-1 increase functional...
Abstract Autologous CAR T therapeutics have recently been approved for use in B-cell malignancies. While responses impressive using CD19 directed cells there has a lack of comparable success at solid tumor antigens. In an effort to address the need effective and durable off-the shelf therapies both hematologic tumors we developed allogeneic targeting CD70 antigen. is expressed malignancies as well cancers such renal cell carcinoma (RCC), while its expression normal tissues restricted subset...