A5010778688- Muscle Physiology and Disorders
- Cardiomyopathy and Myosin Studies
- Cardiovascular Effects of Exercise
- Adipose Tissue and Metabolism
- Cardiovascular Function and Risk Factors
- Nutrition and Health in Aging
- Muscle activation and electromyography studies
- Cardiovascular and exercise physiology
- Muscle metabolism and nutrition
- Cardiac electrophysiology and arrhythmias
- Mitochondrial Function and Pathology
- Cardiac Fibrosis and Remodeling
- Genetics and Physical Performance
- Exercise and Physiological Responses
- Pulmonary Hypertension Research and Treatments
- Metabolism, Diabetes, and Cancer
- Cellular Mechanics and Interactions
- Advanced Proteomics Techniques and Applications
- Sports injuries and prevention
- Cardiac Arrest and Resuscitation
- Meat and Animal Product Quality
- Blood properties and coagulation
- Adipokines, Inflammation, and Metabolic Diseases
- Biotin and Related Studies
- Fuel Cells and Related Materials
University of Wisconsin–Madison
2015-2024
American College of Surgeons
2009
International College of Applied Kinesiology-USA
2003
University of California, Irvine
1990-1993
Single-cell proteomics has emerged as a powerful method to characterize cellular phenotypic heterogeneity and the cell-specific functional networks underlying biological processes. However, significant challenges remain in single-cell for analysis of proteoforms arising from genetic mutations, alternative splicing, post-translational modifications. Herein, we have developed highly sensitive functionally integrated top–down comprehensive single cells. We applied this muscle fibers (SMFs)...
This study examined the relationship between contractile and isomyosin changes occurring in rat soleus (SOL) plantaris (PLAN) muscles after 28 days of hindlimb suspension. SOL from suspended animals exhibited a 45% decline muscle weight compared with controls (P less than 0.05) accompanied by 49% decrease peak twitch tension (Pt) 59% reduction tetanic (Po). Smaller reductions were observed weight, Pt, Po (12, 43, 24%, respectively) for PLAN. Maximal shortening velocity (Vmax) unloaded...
The loss of skeletal muscle function with age, known as sarcopenia, significantly reduces independence and quality life can have significant metabolic consequences. Although exercise is effective in treating sarcopenia it not always a viable option clinically, currently, there are no pharmacological therapeutic interventions for sarcopenia. Here, we show that chronic treatment pan-adiponectin receptor agonist AdipoRon improved male mice by mechanism linked to metabolism tissue remodeling. In...
Cachexia is commonly seen in cancer and characterized by severe muscle wasting, but little known about the effect of cachexia on expression contractile protein isoforms such as myosin. Other causes atrophy shift myosin toward increased fast (type II) isoform expression. We injected mice with murine C-26 adenocarcinoma cells, a tumor cell line that has been shown to cause wasting. Mice were killed 21 days after injection, hindlimb muscles removed. Myosin heavy chain (MHC) light (MLC) content...
Although endurance training has been shown to profoundly affect the oxidative capacity of skeletal muscle, little information is available concerning impact on muscle isomyosin expression across a variety fiber types. Therefore, 10-wk running program (1 h/day, 5 days/wk, 20% grade, 1 mile/h) was conducted ascertain effects in soleus, vastus intermedius (VI), plantaris (PLAN), red and white medial gastrocnemius (RMG WMG), lateralis muscles (RVL WVL). Evidences were noted by presence resting...
Sarcopenia, the loss of skeletal muscle mass and function with advancing age, is a significant cause disability independence in elderly thus, represents formidable challenge for aging population. Nevertheless, molecular mechanism(s) underlying sarcopenia-associated dysfunction remain poorly understood. In this study, we employed an integrated approach combining top-down targeted proteomics mechanical measurements to dissect age-related dysfunction. Top-down proteomic analysis uncovered...
The aim of this study was to contrast competing influences, hypothyroidism and hindlimb suspension, on myosin heavy chain (MHC) expression studied at the protein level mRNA level. Female Sprague-Dawley rats were assigned either normal control (NC), suspended (NS), or hypothyroid (thyroidectomized) (TC) (TS) groups. NS TS animals for 14 days following which myofibrils total RNA purified from muscles. In soleus vastus intermedius (VI), there an increase in type I MHC a decrease IIa both TC...
Sarcopenia, the age-related loss of skeletal muscle mass and strength, is a significant cause morbidity in elderly major burden on health care systems. Unfortunately, underlying molecular mechanisms sarcopenia remain poorly understood. Herein, we utilized top-down proteomics to elucidate sarcopenia-related changes fast- slow-twitch muscles aging rats with focus sarcomeric proteome, which includes both myofilament Z-disc proteins-the proteins that constitute contractile apparatuses. Top-down...
Previous studies have shown that endurance exercise training increases myocardial contractility. We previously described training-induced alterations in contractile function at the cellular level, including an increase Ca 2+ sensitivity of tension. To determine molecular mechanism(s) these changes, oligonucleotide microarrays were used to analyze gene expression profile ventricles from endurance-trained rats. 11-wk treadmill protocol we results increased contractility cardiac myocytes. After...
The heart is known to respond a program of chronic exercise in ways that enhance cardiac function. However, the cellular mechanisms involved training-induced improvements contractile function myocardium are not known. In this study we tested hypothesis increased contractility associated with training due, part, increases Ca(2+) sensitivity steady-state tension. Female Sprague-Dawley rats were randomly divided into sedentary control (C) and exercise-trained (T) groups. T underwent 11 wk...
Determining changes in protein expression and post-translational modifications (PTMs) is crucial for elucidating cellular signal transduction disease mechanisms. Conventional antibody-based approaches have inherent problems such as the limited availability of high-quality antibodies batch-to-batch variation. Top-down mass spectrometry (MS)-based proteomics has emerged most powerful method characterization quantification modifications. Nevertheless, robust methods to simultaneously determine...
Sarcomeric proteins, including myofilament and Z-disk play critical roles in regulating muscle contractile properties. A variety of isoforms post-translational modifications (PTMs) sarcomeric proteins have been shown to be associated with modulation functions the occurrence diseases. Non-human primates (NHPs) are excellent research models for sarcopenia, a disease alterations due their marked similarities humans. However, NHP skeletal not well characterized. To gain deeper understanding...
Abstract Patients undergoing cardiopulmonary bypass procedures require inotropic support to improve hemodynamic function and cardiac output. Current inotropes such as dobutamine, can promote arrhythmias, prompting a demand for improved with little effect on intracellular Ca 2+ flux. Low‐dose carbon monoxide (CO) induces effects in perfused hearts. Using the CO‐releasing pro‐drug, oCOm‐21, we investigated if this results from an increase myofilament sensitivity. Male Sprague Dawley rat left...
This study examined the role of specific types contractile activity in regulating myosin heavy chain (MHC) isoform expression rodent soleus. A combination hindlimb suspension (SN) and two programmed training paradigms, either isometric (ST-IM) or high-load slowly shortening isovelocity activity, were utilized. Both paradigms increased muscle mass compared with SN alone. However, only ST-IM resulted a partial prevention suspension-induced decrease type I MHC. With use fluorescently labeled...
Myocardial function is enhanced by endurance exercise training, but the cellular mechanisms underlying this improved remain unclear. The ability of myocardium to perform external work a critical aspect ventricular function, previous studies myocardial adaptation training have been limited measurements isometric tension or unloaded shortening velocity, conditions in which output zero. We measured force-velocity properties single permeabilized myocyte preparations determine effect on loaded...
Myocardial function is enhanced by endurance exercise training, but the cellular mechanisms underlying this improved remain unclear. A number of studies have shown that characteristics cardiac myocytes vary across width ventricular wall. We previously training alters Ca 2+ sensitivity tension as well contractile protein isoform expression in rat myocytes. tested hypothesis these effects are not uniform wall more pronounced subendocardial (Endo) region myocardium. Female Sprague-Dawley rats...
Myocardial function is enhanced by endurance exercise training, but the cellular mechanisms underlying this improved remain unclear. Exercise training increases sensitivity of rat cardiac myocytes to activation Ca 2+ , and has been shown be highly dependent on sarcomere length. We tested hypothesis that length dependence in myocytes. Female Sprague-Dawley rats were divided into sedentary control (C) exercise-trained (T) groups. The T underwent 11 wk progressive treadmill exercise. Heart...