A5034464802- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Acute Myeloid Leukemia Research
- Health Systems, Economic Evaluations, Quality of Life
- Congenital Heart Disease Studies
- RNA Interference and Gene Delivery
- Retinoids in leukemia and cellular processes
- Antioxidant Activity and Oxidative Stress
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Renal and related cancers
- Frailty in Older Adults
University of Cincinnati Medical Center
2022-2023
University of Wisconsin–Madison
2018-2019
Abstract Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) exhibit a fetal phenotype that limits in vitro and therapeutic applications. Strategies to promote cardiomyocyte maturation have focused interventions on differentiated hPSC-CMs, but this study tests priming of early cardiac progenitor cells (CPCs) with polyinosinic-polycytidylic acid (pIC) accelerate maturation. CPCs were from hPSCs using monolayer differentiation protocol defined small molecule Wnt temporal modulation,...
Human cells have the remarkable capability to regulate protein production by degrading target mRNA two pathways: RNA interference (RNAi) and micro (miRNA). Central these pathways is Argonaute‐2 (Ago‐2). In RNAi pathway, small RNAs derived from viruses are used Ago‐2 slice virus mRNA, protecting infection. miRNA utilizes naturally occurring cellular mRNAs control production. works binding (~22 nucleotide) regulatory (siRNA miRNA) base pairing. attaches phosphate backbone of RNA, that guides...
Cardiomyocytes derived from human induced pluripotent stem cells (hiPS-CMs) hold promise for disease modeling, drug discovery, and therapy, but the challenge remains to create mature cardiomyocytes like those found in adult heart. While groups have increased maturity of hiPS-CMs extended culture with electrical, metabolic, mechanical stimulation, we hypothesized that epigenetic modulation during formation cardiac progenitors (hiPS-CPCs) could enhance their capacity form CMs. We priming...