A novel cell-targeting, pH-sensitive polymeric carrier was employed in this study for delivery of the anticancer drug bortezomib (BTZ) to cancer cells. Our strategy is based on facile conjugation BTZ catechol-containing carriers that are designed be taken up selectively by cells through cell surface receptor-mediated mechanisms. The polymer used as a building block poly(ethylene glycol), which chosen its ability reduce nonspecific interactions with proteins and catechol moiety exploited bind...

On the spot: Carbohydrate microarrays, in concert with surface plasmon resonance, are valuable tools rapid identification of carbohydrate–protein interactions, like those between high-mannose oligosaccharides and cyanovirin-N (see picture). By utilizing chemical methods amenable to current high-throughput screening technologies, high-density carbohydrate microarrays can be prepared screened by using conventional widely accessible DNA microarray instrumentation.

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbon-hydrogen insertions in the reactions of Fischer carbene complexes with ketene acetalsSiu Ling B. Wang, Jing Su, William D. Wulff, and Karst HoogsteenCite this: J. Am. Chem. Soc. 1992, 114, 26, 10665–10666Publication Date (Print):December 1, 1992Publication History Published online1 May 2002Published inissue 1 December 1992https://pubs.acs.org/doi/10.1021/ja00052a089https://doi.org/10.1021/ja00052a089research-articleACS PublicationsRequest...

Peptide and carbohydrate biochips can be assayed with MALDI mass spectrometry. Self-assembled monolayers (SAMs) that are modified ligands provide a platform for identifying selective protein–ligand enzyme–substrate interactions. provides rapid sensitive method detection avoids the need to label protein analytes fluorescent or radioactive tags. The picture shows spectrum of SAM presenting carbohydrate.

This work describes a method for patterning gold substrate with multiple, aligned self-assembled monolayers (SAMs) using light at different wavelengths. It the synthesis and characterization of an alkanethiolate SAM that is photosensitive to both 220 365 nm. A photomask acts as area-selective filter nm, single set exposures these two wavelengths through one photomask, without steps alignment between exposures, can produce three SAMs on substrate. We demonstrate versatility this...

Herein we describe a strategy that combines peptide chips with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to evaluate kinase activities rapidly and semi-quantitatively.We have already shown monolayers of alkanethiolates on gold are wellsuited as substrates for MALDI-TOF MS, could be used measure enzyme perform high-throughput screenings. [1,2] significant aspect this approach-which term SAMDI (self-assembled MALDI) MS-is it avoids the use...

We describe the use of native chemical ligation (NCL) reaction to covalently cross-link soluble polymers into hydrogels. Macromonomers consisting a four-armed poly(ethylene glycol) (PEG) core end-functionalized with either thioester or N-terminal cysteine peptide were designed and synthesized. Upon mixing aqueous solutions macromonomers, rigid hydrogels formed within minutes. The gelation time was affected by choice buffer, pH, polymer concentration, temperature, composition conjugate....

This paper demonstrates that matrix-assisted laser desorption/ionization and time-of-flight mass spectrometry (MALDI-TOF MS) is very well suited for characterizing self-assembled monolayers (SAMs) of alkanethiolates on gold and, in particular, analyzing chemical reactions monolayers. Three examples establish the generality MALDI-TOF this purpose. The first example characterizes Diels−Alder reaction a cyclopentadiene to maleimide groups monolayer. second immobilization glutamine tert-butyl...

This paper reports the use of microfluidic networks (μFNs) to both prepare peptide microarrays and carry out label-free enzyme assays on self-assembled monolayers (SAMs) alkanethiolates gold. A poly(dimethylsiloxane) (PDMS) stamp fabricated with microchannels is used immobilize a linear array cysteine-terminated peptides onto SAMs presenting maleimide groups. The then reapplied SAM in perpendicular direction introduce solutions so that each solution can interact an identical immobilized...

This paper describes a label-free assay for measuring endogenous caspase protease activities in cell lysates. The format, termed SAMDI-MS (self-assembled monolayers matrix assisted laser desorption ionization time-of-flight mass spectrometry), is based on the enzymatic modification of peptides immobilized to monolayer substrates, followed by direct detection products with spectrometry. Monolayers presenting peptide substrates either caspase-3 or -8 were treated lysates from Jurkat cells that...

Limiting the precise study of biochemical impact whole molecule extracellular matrix (ECM) proteins on stem cell differentiation is lack 3D in vitro models that can accommodate many different types ECM. Here we sought to generate such a system while maintaining consistent mechanical properties and supporting survival. To this end, used native chemical ligation cross-link poly(ethylene glycol) macromonomers under mild conditions entrapping ECM (termed composites) cells. Sufficiently low...

Masse als Erkennungskriterium: Mit einer Methode, die Peptidchips und MALDI-TOF-Massenspektrometrie (MS) kombiniert, lassen sich Kinasen simultan analysieren. Dabei werden selbstorganisierte Monoschichten verwendet, einen Satz von Peptiden präsentieren, denen jedes für eine Kinase selektiv ist. Die phosphoryliert das Peptid, was dessen ändert. Dies lässt durch MS-Analyse der Oberfläche Peptid nachweisen.

The reactions of several alkynyl carbene complexes [(CO)5MC(OMe)CCR1, M = Cr, W, R1 Me, Me3Si, Ph, i-Pr, t-Bu) with a variety acyclic enol ethers and ketene acetals [CH2C(OR2)R3, R2 Et, i-Bu, SiMe2t-Bu, R3 H, EtO, p-MeC6H4] are examined. These occur to give [2 + 2] cycloaddition products in all cases except Me3Si where ene predominate. cyclobutenyl produced the cycloadditions undergo rapid electrocyclic ring-opening at room temperature when H butadienyl as isolated products. cyclic derived...

This paper describes a model system for studying the autocatalytic phosphorylation of an immobilized substrate by kinase enzyme. work uses self-assembled monolayers (SAMs) alkanethiolates on gold to present peptide planar surface. Treatment monolayer with Abl results in substrate. The phosphorylated then serves as ligand SH2 adaptor domain and thereby directs activity nearby substrates. directed reaction is intramolecular proceeds faster rate than does initial, intermolecular reaction,...

Rosmarinic acid (RA) is a phenolic originally isolated from the herb medicine Rosmarinus officinalis. The purpose of this study was to identify metabolites RA. RA incubated with human liver microsomes in presence β-nicotinamide adenine dinucleotide phosphate tetrasodium salt and/or uridine diphosphate glucuronic using glutathione (GSH) as trapping agent. After 60-min incubation, samples were analyzed high-resolution liquid chromatography tandem mass spectrometry. Under current conditions, 14...

Utilizing confinement effects, TBTQ-C6 safeguards acetylcholine and butyrylcholine from enzymatic breakdown by AChE BChE through host–guest interactions.

The synthesis of the 2-bromoanthracyclinone 3 containing tetracyclic core menogaril is achieved with a strategy that formulated around benzannulation reaction Fischer carbene complex and an alkyne. This requires 2,5-dimethoxyphenyl bears additional functional group at 4-position nascent 2-bromo substituent in 3. evaluation 4-bromo- 4-trimethysilyl-substituted complexes 1-pentyne revealed was more efficient silyl complex. methoxy acetoxy substituents C-9 position, which begins methoxy-...

Abstract Three chromium(III) 1,2,4-diazaphospholide complexes were prepared: 3,5-di- tert -butyl-1,2,4-diazaphospholide dichloride [( η 2 -3,5- t Bu dp)Cr(THF) Cl ] ( 1 ), 3,5-di-phenyl-1,2,4-diazaphospholide -3,5-Ph and 2,6-bis( N -1,2,4-diazaphosphol-1-yl)pyridine trichloride {[2,6-bis( -1,2,4-diazaphosphol-1-yl)pyridine]CrCl 3 } ). X-ray diffraction analysis has shown that these six-coordinate each have a pseudo-octahedral configuration. Electron paramagnetic resonance (EPR) spectroscopy...

Abstract Solid-liquid phase transfer catalytic elimination reaction for the preparation of acyclic and cyclic ketene acetals has been developed.

A rapid method for the determination of inor-ganic phosphate in serum and milk by an ion-based chromogenic is described. Serum samples were detected directly our system, was also after degreased through centrifugation. By this procedure are not diluted. Mean inorganic concentration found healthy indi-vidual 1.14mmol/L. Values good agreement with those previously re-ported. concentra-tion from foremilk commercial 2.5mmol/L 12.5mmol/L respectively.

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at glance that was extracted from about 100 leading journals. To access of an article which published elsewhere, please select “Full Text” option. The original trackable via the “References”

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at glance that was extracted from about 100 leading journals. To access of an article which published elsewhere, please select “Full Text” option. The original trackable via the “References”

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at glance that was extracted from about 100 leading journals. To access of an article which published elsewhere, please select “Full Text” option. The original trackable via the “References”