A5055845932- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Photochromic and Fluorescence Chemistry
- Neuroscience and Neuropharmacology Research
- Genetic Neurodegenerative Diseases
- Cellular transport and secretion
- Ion channel regulation and function
- Ion Channels and Receptors
- Advanced Fluorescence Microscopy Techniques
- Mitochondrial Function and Pathology
- Cannabis and Cannabinoid Research
- Heart Rate Variability and Autonomic Control
- Epilepsy research and treatment
- Gold and Silver Nanoparticles Synthesis and Applications
- Neurotransmitter Receptor Influence on Behavior
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Neural dynamics and brain function
- Diffusion and Search Dynamics
- Lipid Membrane Structure and Behavior
- Neurological disorders and treatments
- Glycogen Storage Diseases and Myoclonus
- Biochemical Analysis and Sensing Techniques
- Free Radicals and Antioxidants
- Cancer Immunotherapy and Biomarkers
Universitat de Barcelona
2024
Universitat Pompeu Fabra
2017-2021
Columbia University
2018
Institute for Bioengineering of Catalonia
2012-2016
Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision tissue penetration two-photon stimulation have never been investigated these molecules. Here we demonstrate excitation azobenzene-based protein switches versatile strategies enhance photochemical responses. This enables new applications activation neurons astrocytes with cellular subcellular resolution.
TRPV4 cation channel activation by cytochrome P450-mediated derivatives of arachidonic acid (AA), epoxyeicosatrienoic acids (EETs), constitute a major mechanisms endothelium-derived vasodilatation. Besides, mechano/osmosensitivity depends on phospholipase A2 (PLA2) and subsequent production AA EETs. However, the lack evidence for direct interaction EETs with together claims EET-independent mechanical has cast doubts validity this mechanism. We now report: 1) The identification an EET-binding...
Abstract Light-regulated drugs allow remotely photoswitching biological activity and enable plausible therapies based on small molecules. However, only freely diffusible photochromic ligands have been shown to work directly in endogenous receptors methods for covalent attachment depend genetic manipulation. Here we introduce a chemical strategy covalently conjugate photoswitch the of proteins demonstrate its application kainate receptor channel GluK1. The approach is photoswitchable...
Essential for eukaryotic organisms, multiple copies of the exocyst complex tether each secretory vesicle to plasma membrane (PM) in constitutive exocytosis. The higher-order structure (ExHOS) that coordinates action these exocysts remains unexplored. We integrated particle-tracking, super-resolution microscopy and cryo-electron tomography a model time-resolves continuum conformational landscape ExHOS functionally annotates its different conformations. found 7 form flexible ring-shaped...
Stroke-like episodes (SLE) occur in phosphomannomutase deficiency (PMM2-CDG), and may complicate the course of channelopathies related to Familial Hemiplegic Migraine (FHM) caused by mutations CACNA1A (encoding CaV2.1 channel). The underlying pathomechanisms are unknown. We analyze clinical variables detect risk factors for SLE a series 43 PMM2-CDG patients. explore hypothesis abnormal function due aberrant N-glycosylation as potential novel pathomechanism ataxia using whole-cell...
Abstract Optical activation of neurons requires genetic manipulation or the use chemical photoactivators with undesirable side effects. As a solution to these disadvantages, here, we demonstrate optically evoked neuronal activity in mouse cortical acute slices and vivo by nonlinear excitation gold nanoparticles. In addition, this approach stimulate individual epitheliomuscular cells evoke body contractions Hydra vulgaris . To achieve this, low-power pulsed near-infrared at double-wavelength...
A new azobenzene-based photoswitch, 2, has been designed to enable optical control of ionotropic glutamate receptors in neurons via sensitized two-photon excitation with NIR light. In order develop an efficient and versatile synthetic route for this molecule, a modular strategy is described which relies on the use linear fully protected derivative stable basic media. The resulting compound undergoes one-photon trans-cis photoisomerization two different mechanisms: direct its azoaromatic unit...
The development of optical methods to activate neurons with single-cell resolution has enabled systematic mapping inhibitory connections. In contrast, excitatory connections between pyramidal (PCs) been a major challenge due their high densities in cortical tissue and weak stochastic connectivity. Here we present an optogenetic two-photon method mouse neocortical slices by activating PCs the red-shifted opsin C1V1 while recording postsynaptic responses whole-cell configuration. Comparison...
A wide range of light-activated molecules (photoswitches and phototriggers) have been used to the study computational properties an isolated neuron by acting pre postsynaptically. However, new tools are being pursued elicit a presynaptic calcium influx that triggers release neurotransmitters, most them based in calcium-permeable Channelrhodopsin-2 mutants. Here we describe method control exocytosis synaptic vesicles through use light-gated glutamate receptor (LiGluR), which has recently...
The CACNA1A gene encodes the pore-forming α1A subunit of voltage-gated CaV2.1 Ca2+ channel, essential in neurotransmission, especially Purkinje cells. Mutations result great clinical heterogeneity with progressive symptoms, paroxysmal events or both. During infancy, and neuroimaging findings may be unspecific, no dysmorphic features have been reported. We present clinical, radiological evolutionary three patients congenital ataxia, one them carrying a new variant. report structural...
TRPP3 (also called PKD2L1) is a nonselective, cation-permeable channel activated by multiple stimuli, including extracellular pH changes. had been considered candidate for sour sensor in humans, due to its high expression subset of tongue receptor cells detecting sour, along with membership the TRP family known function as sensory receptors. Here, we describe functional consequences two non-synonymous genetic variants (R278Q and R378W) found be under strong positive selection an Ethiopian...