A5039336862- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Retinal Development and Disorders
- Receptor Mechanisms and Signaling
- Molecular Communication and Nanonetworks
- Neuroscience and Neural Engineering
- Light effects on plants
- Neuroscience and Neuropharmacology Research
- Analytical Chemistry and Sensors
- Neural dynamics and brain function
- Polydiacetylene-based materials and applications
- Photosynthetic Processes and Mechanisms
- Oxidative Organic Chemistry Reactions
- Porphyrin and Phthalocyanine Chemistry
University of Göttingen
2022-2023
Universitätsmedizin Göttingen
2022-2023
European Neuroscience Institute Göttingen
2023
Institute for Bioengineering of Catalonia
2016-2023
Universidade Federal de São Paulo
2023
Scripps Research Institute
2023
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2019-2022
German Primate Center
2021-2022
Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103
2022
Barcelona Institute for Science and Technology
2019-2022
Manipulation of neuronal activity using two-photon excitation azobenzene photoswitches with near-infrared light has been recently demonstrated, but their practical use in tissue to photostimulate individual neurons three-dimensional precision hampered by firstly, the low efficacy and reliability NIR-induced photoisomerization compared one-photon excitation, secondly, short cis state lifetime responsive azo switches. Here we report rational design based on theoretical calculations synthesis...
Light-triggered reversible modulation of physiological functions offers the promise enabling on-demand spatiotemporally controlled therapeutic interventions. Optogenetics has been successfully implemented in heart, but significant barriers to its use clinic remain, such as need for genetic transfection. Herein, we present a method modulate cardiac function with light through photoswitchable compound and without manipulation. The molecule, named PAI, was designed by introduction photoswitch...
Abstract Light-regulated drugs allow remotely photoswitching biological activity and enable plausible therapies based on small molecules. However, only freely diffusible photochromic ligands have been shown to work directly in endogenous receptors methods for covalent attachment depend genetic manipulation. Here we introduce a chemical strategy covalently conjugate photoswitch the of proteins demonstrate its application kainate receptor channel GluK1. The approach is photoswitchable...
Photoswitchable neurotransmitters of ionotropic kainate receptors were synthesized by tethering a glutamate moiety to disubstituted C2-bridged azobenzenes, which prepared through novel methodology that allows access diazocines with higher yields and versatility. Because the singular properties these photochromes, photoisomerizable compounds obtained larger thermal stability for their inert cis isomer than biologically activity trans state. This enabled selective neuronal firing upon...
Artificial control of neuronal activity enables the study neural circuits and restoration functions. Direct, rapid, sustained photocontrol intact neurons could overcome limitations established electrical stimulation such as poor selectivity. We have developed fast photoswitchable ligands glutamate receptors (GluRs) to enable in auditory system. The new induced photocurrents untransfected upon covalently tethering endogenous GluRs activating them reversibly with visible light pulses a few...
The ability to control neural activity is essential for research not only in basic neuroscience, as spatiotemporal of a fundamental experimental tool, but also clinical neurology therapeutic brain interventions. Transcranial-magnetic, ultrasound, and alternating/direct current (AC/DC) stimulation are some available means controlled neuromodulation. There light-mediated control, such optogenetics, which has revolutionized neuroscience research, yet its translation hampered by the need gene...
Abstract Optogenetic control of cells is a key life sciences method and promises novel therapies. Here we report on ChReef, an improved variant the channelrhodopsin ChRmine. ChReef offers minimal photocurrent desensitization, unitary conductance 80 fS closing kinetics 30 ms, which together enable reliable optogenetic at low light levels (nano-Joule) with good temporal fidelity allows sustained stimulation. We demonstrate efficient red-light pacing depolarization block ChReef-expressing...
ABSTRACT Artificial control of neuronal activity enables studies neural circuits and restoration function. Direct, rapid, sustained photocontrol intact neurons could overcome shortcomings established electrical stimulation such as poor selectivity. We have developed fast photoswitchable ligands glutamate receptors to establish in the auditory system. The new produced photocurrents untransfected upon covalently tethering endogenous activating them reversibly with visible light pulses few...
Chemical modification with nanometer precision can be used to probe and improve the function of complex molecular entities, from organic materials proteins their assemblies. Using pigment arrangement in photosynthetic light-harvesting as inspiration, we show that photosensitizers located at well-defined distances photoisomerizable units order enhance spectrally shift photoresponses. The approach is demonstrated Channelrhodopsin-2 (ChR2) light-gated ionotropic glutamate receptor (LiGluR), two...
Worldwide 0.5 billion people suffer from disabling hearing loss (HL), mainly caused by a dysfunction of the cochlea. Direct electrical stimulation spiral ganglion neurons (SGNs) enables partial restoration to individuals with severe HL. Yet, wide-spread current each electrode recruits large populations SGNs and limits auditory perception. As light can be better confined in space, Channelrhodopsins (ChRs), utilized optogenetically drive SGNs. For this, it is critical select ChRs fast...
<p>Remote control of physiological functions with light offers the promise unveiling their complex spatiotemporal dynamics in vivo, and enabling highly focalized therapeutic interventions reduced systemic toxicity. Optogenetic methods have been implemented heart, but need genetic manipulation jeopardizes clinical applicability. This study aims at developing, testing validating first light-regulated drug cardiac effects, order to avoid requirement offered by optogenetic methods. A M2...
Remote control of physiological functions with light offers the promise unveiling their complex spatiotemporal dynamics in vivo, and enabling highly focalized therapeutic interventions reduced systemic toxicity. Optogenetic methods have been implemented heart, but need genetic manipulation jeopardizes clinical applicability. This study aims at developing, testing validating first light-regulated drug cardiac effects, order to avoid requirement offered by optogenetic methods. A M2 muscarinic...
Remote control of physiological functions with light offers the promise unveiling their complex spatiotemporal dynamics in vivo, and enabling highly focalized therapeutic interventions reduced systemic toxicity. Optogenetic methods have been implemented heart, but need genetic manipulation jeopardizes clinical applicability. This study aims at developing, testing validating first light-regulated drug cardiac effects, order to avoid requirement offered by optogenetic methods. A M2 muscarinic...
<p>Chemical modification with nanometer precision can be used to probe and improve the function of complex molecular entities, from organic materials proteins their assemblies. Using pigment arrangement in photosynthetic light-harvesting as inspiration, we show that photosensitizers located at well-defined distances photoisomerizable units order enhance spectrally shift photoresponses. The approach is demonstrated Channelrhodopsin-2 (ChR2) light-gated ionotropic glutamate receptor...