A5070965739- Retinal Development and Disorders
- Ocular Surface and Contact Lens
- RNA Interference and Gene Delivery
- Neuroscience and Neural Engineering
- Lipid Membrane Structure and Behavior
- Neurobiology and Insect Physiology Research
- Ion Channels and Receptors
- Zebrafish Biomedical Research Applications
- Neural dynamics and brain function
- Biochemical effects in animals
- Photoreceptor and optogenetics research
- Circadian rhythm and melatonin
- Adipose Tissue and Metabolism
- CCD and CMOS Imaging Sensors
- Allergic Rhinitis and Sensitization
- Gaze Tracking and Assistive Technology
- Thermoregulation and physiological responses
- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Scientific Computing and Data Management
- Neurological Disease Mechanisms and Treatments
- Advanced Memory and Neural Computing
- Neurogenesis and neuroplasticity mechanisms
- Genetics and Physical Performance
- Misinformation and Its Impacts
Universitat de Miguel Hernández d'Elx
2012-2025
Instituto de Neurociencias
2017-2023
Consejo Superior de Investigaciones Científicas
2021-2023
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2012-2021
Instituto de investigación sanitaria y biomédica de Alicante
2021
University of the Basque Country
2014
Cell-fusion-mediated somatic-cell reprogramming can be induced in culture; however, whether this process occurs mammalian tissues remains enigmatic. Here, we show that upon activation of Wnt/β-catenin signaling, mouse retinal neurons transiently reprogrammed vivo back to a precursor stage. This after their spontaneous fusion with transplanted hematopoietic stem and progenitor cells (HSPCs). Moreover, demonstrate damage is essential for cell-hybrid formation vivo. Newly formed hybrids...
Abstract Light-regulated drugs allow remotely photoswitching biological activity and enable plausible therapies based on small molecules. However, only freely diffusible photochromic ligands have been shown to work directly in endogenous receptors methods for covalent attachment depend genetic manipulation. Here we introduce a chemical strategy covalently conjugate photoswitch the of proteins demonstrate its application kainate receptor channel GluK1. The approach is photoswitchable...
Storytelling is a deeply human practice. Through narrative ‐understood as representation of possible world in linguistic and/or visual medium, at whose center protagonists exist temporal and spatial sense (Fludernik, 2009)‐ the audience can immerse different world. Therefore, storytelling be powerful tool with which to engage any person societal relevance scientific project enhance its visibility credibility. This engagement essential for science, even before actual research done. A...
Aims/Purpose: To electrophysiologically characterize the populations of thermosensitive trigeminal neurons that innervate cornea based on their response to cooling. Methods: The activity ganglion was recorded in anesthetized Wistar rats using epoxylite‐insulated W microelectrodes inserted at specific stereotaxic coordinates. Saline drops different temperatures were instilled change corneal temperature, which constantly with a high‐speed thermal probe. Spontaneous without intended stimulation...
The present study aimed to evaluate the incorporation of protamine into niosome/DNA vectors analyze potential application this novel ternary formulation deliver pCMS-EGFP plasmid rat retina. Binary based on and protamine/DNA/niosomes were prepared physicochemically characterized. In vitro experiments performed in ARPE-19 cells. At 1:1:5 protamine/DNA/niosome mass ratio, resulted had 150 nm size, positive charge, spherical morphology, condensed, released, protected DNA against enzymatic...
Perfluorohexyloctane (F6H8) eyedrops have been recently introduced in Europe as a product to treat dry eye disease, based on its ability reduce tear film instability Meibomian gland dysfunction and evaporative although mechanism of action is still unknown. In the present pilot study, we evaluated effects ocular instillation single drop commercial F6H8 20 healthy humans (9 women/11 men), measuring: (a) Corneal surface temperature (CST) from infrared video images; (b) volume using phenol red...
The retina is a very complex neural structure, which contains many different types of neurons interconnected with great precision, enabling sophisticated conditioning and coding the visual information before it passed via optic nerve to higher centers. encoding one basic questions in computational neuroscience also seminal importance field prostheses. In this framework, essential have artificial systems be able function way as similar possible biological retinas. This paper proposes an...
One of the causes nervous system degeneration is an excess glutamate released upon several diseases. Glutamate analogs, like N-methyl-DL-aspartate (NMDA) and kainic acid (KA), have been shown to induce experimental retinal neurotoxicity. Previous results that NMDA/KA neurotoxicity induces significant changes in full field electroretinogram response, a thinning on inner layers, ganglion cell death. However, not all types neurons experience same degree injury response excitotoxic stimulus. The...
Purpose: To study the characteristics of reflex blink evoked by cold and heat stimulation ocular surface (OS), to define contribution TRPM8 process. Methods: The orbicularis oculi muscle electromyography signal (OOemg) temperature (OST; measured with a small thermoprobe placed on cornea) were recorded in anaesthetised rats. OS changes (Δ T ) induced topical instillation 20 μL drops saline solution at different temperatures (15–50°C). Δ area under curve (AUC) duration OOemg signals analysed...
The role of TRPA1 in the thermosensitivity corneal cold thermoreceptor nerve endings was studied young and aged mice. contribution TRPA1-dependent activity to basal tearing thermally-evoked blink also explored. thermoreceptors’ recorded extracellularly (5-month-old) (18-month-old) C57BL/6WT (WT) TRPA1−/− knockout (TRPA1-KO) mice at temperature (34 °C) during cooling (15 heating (45 ramps. response heat stimulation ocular surface rate were measured animals using orbicularis oculi muscle...
Purpose TRPA 1 and TRPM 8 channels provide cold sensitivity to thermoreceptor neurons, being activated by high‐ low‐intensity cooling stimuli respectively. Corneal sensory nerve terminals ( CSNT s) encode both corneal surface temperature reductions tear osmolarity increases associated with evaporation, acting as `dryness detectors′. 8‐dependent activity of s contribute basal tearing is disturbed ageing, while the role 1‐dependent still unknown. In present work, electrical from 1‐ KO mice was...
Behavior is shaped by actions, and actions necessitate motor skills such as strength, coordination, learning. None of the behaviors essential for sustaining life would be possible without ability to transition from one position another. Unfortunately, can compromised in a wide array diseases. Therefore, investigating mechanisms functions at cellular, molecular, circuit levels, well understanding symptoms, causes, progression disorders, crucial developing effective treatments. Mouse models...
Behavior is shaped by actions, and actions necessitate motor skills such as strength, coordination, learning. None of the behaviors essential for sustaining life would be possible without ability to transition from one position another. Unfortunately, can compromised in a wide array diseases. Therefore, investigating mechanisms functions at cellular, molecular, circuit levels, well understanding symptoms, causes, progression disorders, crucial developing effective treatments. Mouse models...