A5080789214- Ocular Surface and Contact Lens
- Corneal surgery and disorders
- Glaucoma and retinal disorders
- Ion Channels and Receptors
- Pain Mechanisms and Treatments
- Neurobiology and Insect Physiology Research
- Olfactory and Sensory Function Studies
- Advancements in Transdermal Drug Delivery
- Corneal Surgery and Treatments
- Ocular and Laser Science Research
- Bee Products Chemical Analysis
- Circadian rhythm and melatonin
- Biochemical effects in animals
- Allergic Rhinitis and Sensitization
- Connexins and lens biology
- Laser Applications in Dentistry and Medicine
- Infrared Thermography in Medicine
- Thermoregulation and physiological responses
- Ergonomics and Musculoskeletal Disorders
- Vestibular and auditory disorders
- Microencapsulation and Drying Processes
- Skin Protection and Aging
- Photoreceptor and optogenetics research
- Urticaria and Related Conditions
- Neuropeptides and Animal Physiology
Universitat de Miguel Hernández d'Elx
2014-2025
Instituto de Neurologia Y Neurocirugia
2025
Universidad Europea
2024
Instituto de Neurociencias
2012-2023
Consejo Superior de Investigaciones Científicas
2001-2023
University of St. Gallen
2015
Hospital Clínic de Barcelona
1991
An alteration in corneal innervation has been described dry eye associated with diabetes mellitus, contact lens use, and LASIK. The purpose of this study was to evaluate whether not related Sjögren's syndrome (NSDE) primary (PSDE) are an the nerves sensation.Twenty-one patients (10 NSDE 11 PSDE) 20 healthy volunteers were studied. Healthy divided into two groups: one younger than 60 years (N<60) other age or older (N> =60). epithelium, stroma, subbasal performed a confocal microscope....
To explore changes in corneal sensitivity that develop patients with dry eye and the relationship between sensibility severity of disease.Experiments were performed 44 42 healthy individuals. Corneal was measured Belmonte noncontact gas esthesiometer. Mechanical (air jets at flow rates from 0 to 200 mL/min, reaching surface 34 degrees C), thermal (cold or warm air subthreshold changing basal temperature +/-1 chemical stimuli containing 0% 50% CO2 rate cornea C) applied center determine...
Eye pain is an unpleasant sensory and emotional experience including sensory-discriminative, emotional, cognitive, behavioral components supported by distinct, interconnected peripheral central nervous system elements. Normal or physiological results of the stimulation noxious stimuli axons trigeminal ganglion (TG) neurons innervating eye. These are functionally heterogeneous. Mechano-nociceptors only excited mechanical forces. Polymodal nociceptors also respond to heat, exogenous irritants,...
Transient receptor potential melastatin 8 (TRPM8) is the best molecular candidate for innocuous cold detection by peripheral thermoreceptor terminals. To dissect out contribution of this cold- and menthol-gated, nonselective cation channel to transduction, we identified BCTC [ N -(4- tert -butylphenyl)-4-(3-chloropyridin-2-yl)piperazine-1-carboxamide] as a potent full blocker recombinant TRPM8 channels. In cold-sensitive trigeminal ganglion neurons mice guinea pig, responses menthol were...
In Brief Dry eye disease (DED) affects >10% of the population worldwide, and it provokes an unpleasant sensation ocular dryness, whose underlying neural mechanisms remain unknown. Removal main lachrymal gland in guinea pigs caused long-term reduction basal tearing accompanied by changes architecture density subbasal corneal nerves epithelial terminals. After 4 weeks, ongoing impulse activity responses to cooling cold thermoreceptor endings were enhanced. Menthol (200 μM) first excited then...
The cornea of human subjects and anaesthetised cats was stimulated with a jet air controlled flow, temperature CO 2 concentration delivered by gas aesthesiometer. In humans, the intensity magnitude various components sensory experience (intensity sensation, degree irritation, burning stinging pain, cold warm sensation) were measured using separate visual analog scales. cats, impulse response to same stimuli recorded from single mechanosensory, polymodal cold‐sensitive corneal fibres in...
purpose. To measure the increase in tear secretion evoked by selective stimulation of different populations sensory receptors cornea and conjunctiva using moderate intense mechanical, chemical, cold stimuli. methods. Six healthy subjects participated study. Tear was measured both eyes Schirmer’s test conducted under control conditions after center temporal with a gas esthesiometer. Mechanical consisted three pulses 3 seconds’ duration warmed air (at 34°C on eye surface) applied at (170...
Summary Ocular discomfort sensations accompanying experimental allergic keratoconjunctivitis are caused by increased responsiveness of corneal polymodal nociceptors, while cold thermoreceptor activity is reduced. Peripheral neural mechanisms underlying the irritation, discomfort, and itch eye response have not been hitherto analyzed. We explored this question recording changes in electrical corneoconjunctival sensory nerve fibers guinea pig after an ocular challenge. Sensitization was...
Purpose.: To produce in guinea pigs a UV-induced keratitis, to analyze the effects of this pathology on corneal nerve activity. Methods.: In anesthetized animals, one eye was exposed 254 nm UV-C radiation (500–1000 mJ/cm2), excised 24 48 hours later and superfused vitro. Nerve impulse activity recorded ciliary filaments or sensory terminals intact UV-irradiated eyes. Impulse response mechanical (von Frey hairs), chemical (98.5% CO2 gas jets), thermal stimulation (cooling from 34°C 20°C;...
Sensory nerve fibers innervating the ocular anterior surface detect external stimuli producing innocuous and painful sensations. Protons are among first mediators released by damaged cells during inflammation, tissue injury, or other chronic ophthalmic conditions. We studied whether acid-sensing ion channels (ASICs) expressed in corneal sensory neurons their roles response to moderate acidifications of pathologies inflammation. Moderate acidic pH (6.6) activated ASIC-like currents neurons,...
Axonal regeneration represents a pivotal aspect of the adult peripheral nervous system (PNS). When an injury occurs, axons are able to regenerate and reestablish connections with their original targets. Although several precision techniques for severing in various non-mammalian model organisms have allowed study dynamics after injury, similar axonal models yet be fully developed mammals. In this study, we introduced novel experimental method laser-induced axotomy single corneal sensory...
Aims/Purpose: To electrophysiologically characterize the populations of thermosensitive trigeminal neurons that innervate cornea based on their response to cooling. Methods: The activity ganglion was recorded in anesthetized Wistar rats using epoxylite‐insulated W microelectrodes inserted at specific stereotaxic coordinates. Saline drops different temperatures were instilled change corneal temperature, which constantly with a high‐speed thermal probe. Spontaneous without intended stimulation...
Background: Treating psychiatric illnesses or influencing mental states with neurofeedback is challenging, likely due to the limited spatial specificity of EEG and complications arising from inadequate signal-to-noise ratio reduction single-trial EEG. Objective: This pilot study aimed investigate feasibility employing a binaural pulse mode-modulation (BPM) device reduce anxiety by self-regulation. We desired determine whether could be significantly reduced regulated using BPM-type systems....
The 5th CORONIS Symposium, held during the 2023 Congress of European Association for Vision and Eye Research (EVER), highlighted growing importance ocular surface innervation in eye disorders. This article summarises insights perspectives shared symposium, which focused on clinical relevance innervation, as well development innovative diagnostic therapeutic approaches pathologies linked to disturbed sensory innervation. Through robust interdisciplinary collaborations, these developments hold...
To determine corneal sensitivity to selective mechanical, chemical, and thermal (heat cold) stimulation in patients with a history of herpes simplex virus (HSV) keratitis.Corneal different modalities stimulus was determined both eyes 16 unilateral HSV keratitis diagnosed 1 12 months before the study. On slit lamp examination, 13 HSV-affected showed scarring or opacities, three had no signs previous keratitis. Corneal Belmonte gas esthesiometer. Mechanical, heat, cold stimuli were applied on...
FOLLOWING nerve injury, modified somatic ion channels may underlie ectopic activity in axotomized A-type neurones dorsal root ganglia (DRGs) leading to abnormal pain signalling. Using intracellular microelectrodes both vivo and vitro, action potentials (APs) were recorded rat DRG classified by axonal conduction velocity. After lesions L5 spinal or sciatic nerves, APs Aα/β Aδ cells wider, those more frequently showed inflections during repolarization, than undamaged ganglia. AP amplitudes...