A5000484003- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Photoreceptor and optogenetics research
- Nerve injury and regeneration
- melanin and skin pigmentation
- RNA Interference and Gene Delivery
- Neuroscience and Neuropharmacology Research
- Ocular Surface and Contact Lens
- Axon Guidance and Neuronal Signaling
- Retinal and Optic Conditions
- Ocular and Laser Science Research
- Aquaculture disease management and microbiota
- Neurogenesis and neuroplasticity mechanisms
- Lipid Membrane Structure and Behavior
- Circadian rhythm and melatonin
- Advanced Fluorescence Microscopy Techniques
- Physiological and biochemical adaptations
- Neurological Disorders and Treatments
- Intraocular Surgery and Lenses
- Invertebrate Immune Response Mechanisms
- Cerebral Venous Sinus Thrombosis
- Adenosine and Purinergic Signaling
- Neuroscience and Neural Engineering
Instituto Murciano de Investigación Biosanitaria
2015-2025
Universidad de Murcia
2015-2025
Instituto de Salud Carlos III
2014
Universitat de Miguel Hernández d'Elx
2002-2003
Ocular hypertension is a major risk factor for glaucoma, neurodegenerative disease characterized by an irreversible decrease in ganglion cells and their axons. Macroglial microglial appear to play important role the pathogenic mechanisms of disease. Here, we study effects laser-induced ocular (OHT) macroglia, microglia retinal (RGCs) eyes with OHT (OHT-eyes) contralateral two weeks after lasering.Two groups adult Swiss mice were used: age-matched control (naïve, n=9); lasered (n=9). In...
Glaucomatous optic neuropathy, a leading cause of blindness, can progress despite control intraocular pressure - currently the main risk factor and target for treatment. Glaucoma progression shares mechanisms with neurodegenerative disease, including microglia activation. In present model ocular hypertension (OHT), we have recently described morphological signs retinal activation MHC-II upregulation in both untreated contralateral eyes OHT eyes. By using immunostaining, sought to analyze...
In the mouse model of unilateral laser-induced ocular hypertension (OHT) microglia in both treated and normotensive untreated contralateral eye have morphological signs activation up-regulation MHC-II expression comparison with naïve. brain, rod-like align to less-injured neurons an effort limit damage. We investigate whether: i) microglial is secondary laser injury or a higher IOP and; ii) presence related OHT. Three groups mice were used: age-matched control (naïve, n=15); two lasered:...
Abstract Microglial activation is associated with glaucoma. In the model of unilateral laser-induced ocular hypertension (OHT), time point at which inflammatory process peaks remains unknown. Different points (1, 3, 5, 8, and 15 d) were compared to analyze signs microglial both in OHT contralateral eyes. eyes, was detected all retinal layers analyzed, including: i) increase cell number outer segment photoreceptor layer plexiform (only eyes) from 3 d onward; ii) soma size 1 iii) retraction...
Purpose.: To analyze the effects of different levels intraocular pressure (IOP) in macroglia ocular hypertension (OHT) and contralateral eyes at 3 weeks after laser photocoagulation compare these with age-matched control rats. Methods.: Adult Sprague-Dawley rats were divided into an (naive) group OHT group. Retinas processed as whole mounts immunostained GFAP for analysis retinal macroglia. Results.: The area retina occupied by astrocytes (AROA) was quantified. immunostaining showed common...
To investigate the long-term effects of laser-photocoagulation (LP)-induced ocular hypertension (OHT) in innermost and outermost (outer-nuclear outer segment)-retinal layers (ORL). OHT was induced left eye adult rats. ganglion cell layer (GCL) wholemounts were examined at 1, 3 or 6 months using Brn3a-immunodetection to identify retinal cells (RGCs) DAPI-staining detect all nuclei this layer. study LP on ORL up months, retinas were: i) fresh extracted quantify levels rod-, S- L-opsin; ii) cut...
Signaling mediated by cytokines and chemokines is involved in glaucoma-associated neuroinflammation the damage of retinal ganglion cells (RGCs). Using multiplexed immunoassay immunohistochemical techniques a glaucoma mouse model at different time points after ocular hypertension (OHT), we analyzed (i) expression pro-inflammatory cytokines, anti-inflammatory BDNF, VEGF, fractalkine; (ii) number Brn3a+ RGCs. In OHT eyes, there was an upregulation IFN-γ days 3, 5, 15; IL-4 1, 7 IL-10 3 5...
To study the effects of ocular hypertension (OHT) on visual system C57BL/6 pigmented mice, limbal and episcleral veins left eye were laser photocoagulated (LP). LP increased intraocular pressure during first five days (d), reaching basal values at 7d. investigate effect OHT retinal ganglion cell (RGC) retrograde axonal transport, hydroxistilbamidine methanesulfonate (OHSt) was applied to both superior colliculi (SCi) retinas dissected 2 or 4 weeks after LP. determine RGC survival, these same...
The present study aimed to evaluate the incorporation of protamine into niosome/DNA vectors analyze potential application this novel ternary formulation deliver pCMS-EGFP plasmid rat retina. Binary based on and protamine/DNA/niosomes were prepared physicochemically characterized. In vitro experiments performed in ARPE-19 cells. At 1:1:5 protamine/DNA/niosome mass ratio, resulted had 150 nm size, positive charge, spherical morphology, condensed, released, protected DNA against enzymatic...