A5074002784- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Advanced Fluorescence Microscopy Techniques
- Endoplasmic Reticulum Stress and Disease
- Lipid Membrane Structure and Behavior
- Advanced Electron Microscopy Techniques and Applications
- RNA Research and Splicing
- Protein Structure and Dynamics
- Nuclear Structure and Function
- Enzyme Structure and Function
- Diffusion and Search Dynamics
- Health and Medical Education
- Erythrocyte Function and Pathophysiology
- Galectins and Cancer Biology
- RNA modifications and cancer
- Bioinformatics and Genomic Networks
- Higher Education Teaching and Evaluation
- Innovations in Medical Education
Universitat Pompeu Fabra
2020-2024
Essential for eukaryotic organisms, multiple copies of the exocyst complex tether each secretory vesicle to plasma membrane (PM) in constitutive exocytosis. The higher-order structure (ExHOS) that coordinates action these exocysts remains unexplored. We integrated particle-tracking, super-resolution microscopy and cryo-electron tomography a model time-resolves continuum conformational landscape ExHOS functionally annotates its different conformations. found 7 form flexible ring-shaped...
Abstract Multisubunit Tethering Complexes (MTCs) are a set of conserved protein complexes that tether vesicles at the acceptor membrane. Interactions with other components trafficking machinery regulate MTCs through mechanisms partially understood. Here, we systematically investigate interactome regulates MTCs. We report P4‐ATPases, family lipid flippases, interact participate in anterograde and retrograde transport Golgi, such as TRAPPIII. use P4‐ATPase Drs2 paradigm to mechanism biological...
Abstract Summary Structural knowledge of protein assemblies in their physiological environment is paramount to understand cellular functions at the molecular level. Protein interactions from Imaging Complexes after Translocation (PICT) a live-cell imaging technique for structural characterization macromolecular living cells. PICT relies on measurement separation between labelled molecules using fluorescence microscopy and cell engineering. Unfortunately, required computational tools extract...
Abstract Structural knowledge of protein assemblies in their physiological environment is paramount to understand cellular functions at the molecular level. Protein interactions from Imaging Complexes after Translocation (PICT) a live-cell imaging technique for structural characterization macromolecular living cells. PICT relies on measurement separation between labelled molecules using fluorescence microscopy and cell engineering. Unfortunately, required computational tools extract...
The human interactome is composed of around half a million interactions according to recent estimations and it only for small fraction those that three-dimensional structural information available. Indeed, the coverage very low given complexity time-consuming requirements solving protein structures this problem will remain foreseeable future. Structural models, or predictions, complexes can provide valuable when experimentally determined 3D are not Here we present CM2D3, relational database...
ABSTRACT Although the structure of exocyst has been successfully resolved by cryo-electron microscopy, multiple studies showed that function requires transient interaction with additional proteins. Unfortunately, exocyst-interacting network could not be collectively reconstituted, challenging understanding how complex is coordinated within proteins involved in exocytosis. In a previous work, we described an approach combines Protein interactions from Imaging Complexes after Translocation...
El Plan de Acción Tutorial la Facultad Medicina y Ciencias Vida Universidad Pompeu Fabra es un programa consolidado que se inició en el curso 1999-2000. Este facilita adaptación del estudiante al entorno universitario permite seguimiento orientación individualizada alumnado. En contexto actual, con implementación nuevo grado Medicina, surge necesidad actualizar mejorar calidad programa. esta línea, ha evaluado su funcionamiento actual a través una encuesta para estudiantes profesores. Como...
Autophagy requires a tightly controlled and adjustable subcellular distribution of Atg9. Although the machinery that regulates transport Atg9-positive vesicles has attracted much interest, mechanism controlling Atg9 trafficking beyond standard laboratory conditions remains poorly understood. Recently, we demonstrated lipid flippase Drs2 participates in to phagophore assembly site, this function becomes necessary as temperature drops. Drs2, through an I(S/R)TTK motif nested conserved...
Abstract Multisubunit Tethering Complexes (MTCs) are a set of conserved protein complexes that tether transported vesicles at the acceptor membrane. Interactions with other components trafficking machinery regulate MTCs through mechanisms just partially understood. Here we systematically investigate interactome regulates function MTCs. We found P4-ATPases, family lipid transporters involved in biogenesis vesicles, interact participate anterograde and retrograde transport Golgi, such as...