A5057818620- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Chromosomal and Genetic Variations
- Sperm and Testicular Function
- Drug Transport and Resistance Mechanisms
- Sexual Differentiation and Disorders
- Animal Genetics and Reproduction
- Evolution and Paleontology Studies
- Genomics and Chromatin Dynamics
- Multiple Myeloma Research and Treatments
- Liver Disease and Transplantation
- Genetic Mapping and Diversity in Plants and Animals
- Pharmacological Effects and Toxicity Studies
- Reproductive Biology and Fertility
- Neonatal Health and Biochemistry
- Genetic diversity and population structure
- Developmental Biology and Gene Regulation
- Animal Behavior and Reproduction
- Metabolism and Genetic Disorders
- Wildlife Ecology and Conservation
- Folate and B Vitamins Research
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Physiological and biochemical adaptations
- Cancer-related molecular mechanisms research
- Pediatric Hepatobiliary Diseases and Treatments
Universidad de Granada
2013-2024
Universitat Autònoma de Barcelona
2019-2024
Instituto de Investigación Biomédica de Salamanca
2012-2020
Universidad de Salamanca
2003-2018
Universidad de Murcia
2014
University of Aberdeen
2009
Hospital Nacional de Niños
2007
Universidad de Costa Rica
2007
Central University of Venezuela
2007
Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias
2006
The new concept of mammalian sex maintenance establishes that particular key genes must remain active in the differentiated gonads to avoid genetic reprogramming, as described adult ovaries after Foxl2 ablation. Dmrt1 plays a similar role postnatal testes, but mechanism testis remains mostly unknown. Sox9 and Sox8 are required for male fertility, their has not been investigated. Here we show ablation Sertoli cells adult, fertile Sox8-/- mice, testis-to-ovary reprogramming occurs...
Summary In 112 untreated myeloma patients we have analysed the immunophenotype of plasma cells both by immunofluorescence (IF) and immunocytochemistry (APAAP). Both techniques yielded similar results pointing to aH important degree heterogeneity in antigenic expression not only between different but also within same patient. The CD38 Han‐PCl antigens (Ags) was almost constant (> 90% positive cases), while CD9 detected 66% cases. On other hand, less than one third were for CD 10, CD20...
In mammals, sex differentiation depends on gonad development, which is controlled by two groups of sex-determining genes that promote one gonadal and antagonize the opposite one. SOX9 plays a key role during testis development in all studied vertebrates, whereas it kept inactive XX at critical time determination, otherwise, ovary-to-testis reversal occurs. However, molecular mechanisms underlying repression Sox9 beginning ovarian as well other important aspects organogenesis, remain largely...
In this study the incidence of DNA aneuploidy in a large series untreated multiple myeloma (MM) patients was assessed order to determine its clinical and prognostic significance. A total 156 MM were included study. measurements performed all cases at diagnosis using two different flow cytometry methods: (1) propidium iodide (PI) staining on isolated nuclei, (2) CD38/PI double whole cells. The ploidy status correlated with most relevant haematological disease characteristics. From analysed,...
The aim of this study was to analyse the expression NK‐associated antigens in both peripheral blood and bone marrow lymphocytes from a large series newly diagnosed multiple myeloma patients. 112 patients with untreated (MM) were included study. 36 sex‐ age‐matched healthy volunteers used as controls for (PB) studies 14 (BM) studies. Simultaneous stainings CD3/CD56, CD2/CD16 CD8/CD57 monoclonal antibodies systematically performed PB CD3/CD56 BM order their relationship clinical biological...
In an attempt to understand the hepatotoxicity associated with immunosuppressive therapy cyclosporin A, we investigated effects of acute A administration on biliary secretion, serum bile acid and bilirubin levels histological changes in hepatic parenchyma anesthetized male Wistar rats. The animals were divided into three experimental groups that received equal volumes (1 ml, intravenously) physiological saline (controls), vehicle (a fat emulsion, Intralipid, mixed absolute ethanol) or...
Apoptosis and cell proliferation are two important cellular processes known to be involved in the normal functioning of testis nonseasonally breeding mammals, but there is some controversy concerning their roles gonads males from seasonally species. We have studied apoptosis testes Iberian mole (Talpa occidentalis), a species showing strict seasonal reproduction pattern. Both females sexually active during winter completely inactive summer, with transitional periods, autumn spring. Adult...
Abstract Mammalian sex determination is controlled by antagonistic gene cascades operating in embryonic undifferentiated gonads. The expression of the Y-linked SRY sufficient to trigger testicular pathway, whereas its absence XX embryos leads ovarian differentiation. Yet, potential involvement non-coding regulation this process remains unclear. Here we show that deletion a single microRNA cluster, miR-17 ~ 92 , induces complete primary male-to-female reversal XY mice. Sry delayed knockout...
ABSTRACT We investigated the origin of XX sex reversal in insectivorous mole Talpa occidentalis. Cytogenetic, histological and hormonal studies indicate that all individuals analyzed from two different populations are true hermaphrodites, with ovotestes. This suggests may be norm this species. The inter-sexes functional fertile females trait is transmitted maintained population. Intersexes lack Y chromosome gene SRY (sex determining region gene), shown to testis gene. These results suggest...
The zinc-finger transcription factor SLUG (SNAI2) triggers epithelial–mesenchymal transitions (EMTs) and plays an important role in the developmental processes. Here, we show that is expressed white adipose tissue (WAT) humans its expression tightly controlled during adipocyte differentiation. Slug-deficient mice exhibit a marked deficiency WAT size, Slug-overexpressing (Combi-Slug) increase size. Consistent with vivo data, mouse embryonic fibroblasts (MEFs) showed dramatically reduced...
In males of seasonally breeding species, testes undergo a severe involution at the end season, with major volume decrease due to massive germ-cell depletion associated photoperiod-dependent reduced levels testosterone and gonadotropins. Although it has been repeatedly suggested that apoptosis is principal effector testicular regression in vertebrates, recent studies do not support this hypothesis some mammals. The purpose our work discover alternative mechanisms testis these species. paper,...