A5023410987- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genetic factors in colorectal cancer
- Gallbladder and Bile Duct Disorders
- Glioma Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- Lung Cancer Treatments and Mutations
- SARS-CoV-2 detection and testing
- Epigenetics and DNA Methylation
- Renal cell carcinoma treatment
- Pediatric Hepatobiliary Diseases and Treatments
- Cervical Cancer and HPV Research
- Sarcoma Diagnosis and Treatment
- Biosensors and Analytical Detection
- Lung Cancer Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Radiomics and Machine Learning in Medical Imaging
- Fibroblast Growth Factor Research
- Bladder and Urothelial Cancer Treatments
- Esophageal Cancer Research and Treatment
- Medical Imaging and Pathology Studies
- Endometrial and Cervical Cancer Treatments
- Renal and related cancers
- Neuroendocrine Tumor Research Advances
Mayo Clinic in Arizona
2016-2025
Mayo Clinic
2016-2025
Mayo Clinic in Florida
2017-2025
WinnMed
2007-2025
Klinikum Dortmund
2024
Witten/Herdecke University
2024
University of Minnesota Rochester
2014-2022
American Society for Radiation Oncology
2022
Jazz Pharmaceuticals (United States)
2022
PharmaMar (Spain)
2022
BACKGROUND AND AIM: The aim of this study was to assess the relative sensitivities and specificities fluorescence in situ hybridization (FISH) routine cytology for detection malignancy biliary tract strictures. METHODS: Bile duct brushing aspirate specimens were collected from 131 patients being evaluated possible malignant bile Both specimen types assessed by FISH but only cytology. assay used a mixture fluorescently-labeled probes centromeres chromosomes 3, 7, 17 chromosomal band 9p21...
Advanced cholangiocarcinoma continues to harbor a difficult prognosis and therapeutic options have been limited. During the course of clinical trial whole genomic sequencing seeking druggable targets, we examined six patients with advanced cholangiocarcinoma. Integrated genome-wide transcriptome sequence analyses were performed on tumors from advanced, sporadic intrahepatic (SIC) identify potential therapeutically actionable events. Among somatic events captured in our analysis, uncovered...
Purpose Molecular diagnostic testing has become an integral part of the evaluation patients with metastatic colorectal cancer (CRC). Expanded mutational testing, such as next-generation sequencing (NGS), often identifies mutations unclear clinical or prognostic implications. One example is BRAF that occur outside codon 600 (non-V600 mutations). Methods We conducted this multicenter, retrospective cohort study to characterize clinical, pathologic, and survival implications non-V600 in CRC....
Small intestine neuroendocrine tumors (SI-NETs) are the most common malignancy of small bowel. Several clinical trials target PI3K/Akt/mTOR signaling; however, it is unknown whether these or other genes genetically altered in tumors. To address underlying genetics, we analyzed 48 SI-NETs by massively parallel exome sequencing. We detected an average 0.1 somatic single nucleotide variants (SNVs) per 106 nucleotides (range, 0-0.59), mostly transitions (C>T and A>G), which suggests that stable...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its resultant clinical presentation, disease 2019 (COVID-19), is an emergent cause of mortality worldwide. Cardiac complications secondary to this infection are common; however, the underlying mechanisms such remain unclear. A detailed cardiac evaluation a series individuals with COVID-19 undergoing postmortem provided, 4 aims: (1) describe pathological spectrum myocardium; (2) compare alternate viral illness; (3) investigate...
BACKGROUND AND AIMS Standard techniques for evaluating bile duct strictures have poor sensitivity detection of malignancy. Newer imaging modalities, such as intraductal ultrasound (IDUS), and advanced cytologic techniques, digital image analysis (DIA) fluorescence in situ hybridization (FISH), identify chromosomal abnormalities, may improve while maintaining high specificity. Our aim was to prospectively evaluate the accuracy these patients with indeterminate biliary strictures. METHODS...
Mucinous ovarian tumors represent a distinct histotype of epithelial cancer. The rarest (2-4 % carcinomas) the five major histotypes, their genomic landscape remains poorly described. We undertook hotspot sequencing 50 genes commonly mutated in human cancer across 69 mucinous tumors. Our goals were to establish overall frequency cancer-hotspot mutations large cohort, especially those previously thought be “RAS-pathway alteration negative”, using highly-sensitive next-generation as well...
Distinguishing independent primary tumors from intrapulmonary metastases in non-small-cell carcinoma remains a clinical dilemma with significant implications. Using next-generation DNA sequencing, we developed chromosomal rearrangement-based approach to differentiate multiple metastasis.Tumor specimens patients known and metastatic lesions were used for lineage test development, which was then applied multifocal tumors. Laser capture microdissection performed separately each tumor. Genomic...
// Ferga C. Gleeson 1 , Sarah E. Kerr 2 Benjamin R. Kipp Jesse S. Voss Douglas M. Minot Zheng Jin Tu 3 Michael Henry Rondell P. Graham George Vasmatzis 4 John Cheville Konstantinos N. Lazaridis 1, J. Levy Division of Gastroenterology & Hepatology, Mayo Clinic Rochester, MN, USA Department Laboratory Medicine Pathology, Biomedical Statics Informatics, Health Sciences Research, Center for Individualized Medicine, Clinic, Correspondence to: Gleeson, email: gleeson.ferga@mayo.edu Keywords:...
Circulating tumor cells (CTCs) in blood are associated with poor survival of patients breast, prostate, or colon cancer. We hypothesized that CTCs cholangiocarcinoma (CCA). Eighty‐eight CCA were prospectively enrolled at Mayo Clinic Rochester between June 2010 and September 2014. The CellSearch system by Veridex was used for detection peripheral blood. Associations CTC, patient characteristics, examined using the Cox's proportional hazards model. Fifteen (17%) positive CTC ≥2 8 (9%) ≥5....
COVID-19 is a multi-system disorder with high variability in clinical outcomes among patients who are admitted to hospital. Although some cytokines such as interleukin (IL)-6 believed be associated severity, there no early biomarkers that can reliably predict more likely have adverse outcomes. Thus, it crucial discover predictive markers of serious complications.
BACKGROUNDA patient-derived organoid (PDO) platform may serve as a promising tool for translational cancer research. In this study, we evaluated PDO's ability to predict clinical response gastrointestinal (GI) cancers.METHODSWe generated PDOs from primary and metastatic lesions of patients with GI cancers, including pancreatic ductal adenocarcinoma, colorectal cholangiocarcinoma. We compared PDO the observed donor same treatments.RESULTSWe report an approximately 80% concordance rate between...
We evaluated fluorescence in situ hybridization (FISH) for assessing the response to therapy patients with superficial bladder cancer receiving bacillus Calmette-Guerin or other intravesical therapies.A total of 37 were enrolled this study. Urine specimens collected FISH analysis just prior first 31 cases and within 2 months following last 37. was done using UroVysion probe set (Abbott Laboratories, Abbott Park, Illinois) results considered positive if 5 more cells demonstrated polysomy....
A polysomy fluorescence in situ hybridization (FISH) result a pancreatobiliary brushing from patient with primary sclerosing cholangitis (PSC) is very worrisome for carcinoma. However, treatment not recommended unless verified by corroborative evidence of malignancy because less than perfect test specificity this population. The aim study was to evaluate the clinical outcome PSC patients serial FISH results.Patients underwent endoscopic retrograde cholangiopancreatography brushings when...
It is broadly accepted that the false positive (FP) rate for endoscopic ultrasound fine needle aspiration (EUS FNA) 0-1%. was hypothesised FP and suspicious (FS) rates EUS FNA are greater than reported. A study undertaken to establish root cause of discordant interpretation.Using a prospectively maintained database, cytohistological examinations from 30 July 1996 31 December 2008 were identified retrospectively.Tertiary referral centre.Discordant defined by or cytology in absence malignancy...