We have previously shown that, while the intrinsic quality of oocyte is main factor affecting blastocyst yield during bovine embryo development in vitro, postfertilization culture conditions. Therefore, any improvement blastocysts produced vitro likely to derive from modification The objective this study was examine effect presence or absence serum and concentration BSA period on 1) cleavage rate, 2) kinetics development, 3) yield, 4) quality, as assessed by cryotolerance gene expression...

A high intramuscular fat content characterizes Wagyu (WY) cattle breed. Our objective was to compare beef from WY, WY-by-Angus, or Wangus (WN) steers with European, Angus-by-Charolais-Limousine crossbred (ACL), considering metabolic biomarkers pre-slaughtering and nutritional characteristics, including health-related indexes of the lipid fraction. The fattening system olein-rich diets no exercise restriction included 82 steers, 24 29 WN, ACL. slaughter ages weights were (median interquartile...

ABSTRACT The influence of estrus and double ovulations Holstein heifers on a 5d‐Co‐synch 72 h protocol during summer was explored. Cycling the 5d‐Co‐72 synchronisation were randomised to carry progesterone device (116) from day 0 5 or not (97), them 100 84 underwent fixed‐time artificial insemination (FTAI). Another 70 showed before first GnRH inseminated. Heifers pregnant 34% (33/97) without device, 44.0% (51/116) with 44.3% (31/70) inseminated after estrus. Pregnancy per (P/AI) tended be...

The aim of this study was to explore how some reproductive methodologies may affect the sex ratio. We first confirmed association between maturation stage bovine oocytes at time in vitro fertilisation (IVF) and ratio vitro-derived embryos. Secondly, we studied whether insemination, prior or after ovulation, could alter sheep. In experiment, were matured for 16 h; then either fertilised immediately extrusion polar body IVF delayed 8 h. proportion cleaving embryos their development 8-cell...

Abstract The effect of glucose in the medium used during vitro culture on both cell death by apoptosis and sex ratio bovine blastocysts derived from vitro‐matured vitro‐fertilized oocytes was evaluated. Oocytes were matured, inseminated, cultured mSOF with 10% FCS or without supplementation. Exposure to high concentrations (10, 20, 30 mM) embryo development zygote blastocyst resulted a decrease number cells per an increase frequency apoptotic cells. A significantly higher proportion females...

Effects of hyaluronan (HA), BSA, and FCS on in vitro development, ultrastructure, mRNA transcription four developmentally important genes: apoptosis (Bax), oxidative stress (SOX), growth factor (IGF-II), cell-to-cell adhesion (Ecad) were examined. Two biological origin HA, Hylartil Hyonate one produced by fermentation (f-HA) MAP-5 tested. Embryos cultured SOF medium with 0.4% BSA or 10% FCS. HA was added 96 hr post insemination (pi) to half the embryos from each culture group. Embryo...

Offspring gender preselection has applications of considerable economic, health and ecological interest. In this study we analysed modifications the percentages spermatozoa bearing Y X chromosomes when semen samples are submitted to a double swim-up technique as possible method for producing embryos known sex with in vitro fertilisation protocols. As an initial experiment provide accurate evaluation determined incidence natural deviations primary ratio between bulls or ejaculates, analysing...

Hyaluronan (HA) is glycosaminoglycan that present from the start of embryonic development and its role concentration increases with embryo development. The objective this study was to evaluate if presence HA in TCM-199 culture medium had an effect on quality bovine embryos. There no total number zygotes developing blastocysts day 7, however more expanded hatched blastocyst stages were observed days 8 9 group supplemented (p<0.05). Following freeze/thawing, significantly (p<0.05) embryos...

CASA kinetic parameters are often evaluated in a diagnostic centre. How storage conditions affect ejaculates up to evaluation is unclear. We assessed, 25 commercial bulls electroejaculated the field, impact of time until (0–2 h, 4–6 and 24 h post-ejaculation), holding temperature (5 °C vs. room temperature), extender (AndroMed®, BIOXcell® or INRA96®) on parameters. Total progressive motility, VCL, VAP, ALH, BCF, STR, LIN, WOB were assessed. preserved for post-ejaculation, except AndroMed®....

Seminal parameters can be evaluated in situ, or samples delivered to a diagnostic centre. How storage conditions affect ejaculates up evaluation is unclear. We assessed, 25 commercial bulls electroejaculated the field, impact of time until (0–2 h, 4–6 and 24 h post-ejaculation), holding temperature (5 °C vs. room temperature), extender (AndroMed®, BIOXcell® INRA96®) on semen quality. Acrosome integrity, sperm viability morphology, CASA-total progressive motility, pH, colony-forming units...

Abstract The aim of this study was to assess whether vulvar morphometric changes occurring in female pigs during proestrus and oestrus could be objective, accurate predictive indicators the onset thus performed artificial inseminations at most appropriate time. For that purpose, pictures vulvas from 60 hyperprolific females (30 gilts 30 sows) were taken once a day. Vulva measurements (area, perimeter, length width) on these using image processing ImageJ software. Gilts sows showed...

Basic characteristics of European bison (Bison bonasus) semen were described and the efficacies two extenders—Triladyl, containing egg yolk, a synthetic extender, soybean lipids—were tested for cryopreservation. Seven ejaculates collected by electroejaculation from 10-yr-old, bull. Each ejaculate was diluted at 37°C to final concentration 200 × 106 sperm/ml with Triladyl or extender. Extended samples frozen according standard bull freezing protocol. After 2 wk storage, one straw each...

Contents The main aim of this study was to document the prevalence chromosomal aberrations found date on pig population in Spain, a country which production sector has critical role, being fourth world and second one within European Union. total number animals studied 849, founded frequency carrier pigs with alterations 3.8%. When only structural were considered, males 3.3%. This percentage is far from 0.5% boars that been estimated France, where there systematic cytogenetic screening future...

Abstract When Chu‐Lin, a male giant panda (studbook #249), died at Madrid Zoo, his reproductive tract was removed 4 hr postmortem and the epididymal spermatozoa were collected. Extended sperm kept 5°C for hr, loaded into straws, frozen 7 min in liquid nitrogen vapor before straws plunged nitrogen. Two thawed evaluated. Sperm motility assessed fresh, refrigerated, (75%, 60%, 35%, respectively). viability acrosome status estimated using triple‐stain technique (TST). The results showed 33% live...

Japanese Black (Wagyu) cattle produce high-quality beef. However, whether Wagyu steers can be profitably raised under conditions different than the traditional ones remains unclear. From 2018 to 2020, we 262 purebred steers, 103 Wagyu-by-Angus (Wangus) crossbred and 43 Angus-by-European (ACL) on a Spanish farm with high welfare standards locally sourced, high-olein diet. Factors factors’ interactions impacting steer growth were analyzed using generalized linear models. ACL grew faster other...

Fecal 17β-estradiol and progestogens excretion was monitored in adult, female cheetahs (Acinonyx jubatus; n = 2), ZGG-12301 (born 3 April 1993), gonadotrophin treated ZGT-3301, 19 August nontreated, for 120 days using commercially available plate enzyme immunoassay kits prepared human serum or plasma. There were significant differences (P < 0.001) between baseline peak concentrations of both hormone measures. Female ZGG-12301, which conceived, but this pregnancy resulted an unobserved...

Analysis of reproductive hormones in fecal samples is necessary for the noninvasive monitoring status free-ranging species. The aim present study was to establish an easy method monitor wild ungulate females. Feces were collected daily, weekly, or three four times a week directly from soil period ranging 1 9.8 mo. Fecal estradiol and progestagens monitored nine females (Barbary sheep, Ammotragus lervia [n = 3]; European bison, Bison bonasus 1]; auroch, Bos taurus primigenius 2]; sitatunga,...

The aim was to assess the reproductive efficiency of different techniques used preserve spermatozoa in artificial insemination semen doses (AI-doses) by evaluating refrigeration at 15°C, cryopreservation and encapsulation. Forty-two hyperprolific sows were treated with buserelin inseminated once a single fixed time. fertility rate, embryonic vesicles viability early mortality (arrested conceptuses) evaluated post-mortem 24th day pregnancy, analysed order effectiveness each proposed...

Sperm quality decreases over time, so bull semen may need to be preserved after field collection. However, the effect of handling such samples from commercial farms and placing them in very short-term storage has not been elucidated. Therefore, ejaculate 25 bulls 1 dairy 14 beef cattle were collected under farm conditions evaluated for during first two hours Two extenders (AndroMed® BIOXcell®) different temperatures (5 °C room temperature) used evaluate influence on sperm kinetics ejaculates...

This experiment was designed to test the efficacy of 2 different preparation protocols zwitteronic soyabean-origin lipids for production lipidsglycerol liposomes use in bull semen cryopreservation. Lipids were prepared at 10% concentration Tris buffer by 1. highpressure homogenization (Panda 2K, Parma, Italy) and then 8% glycerol added, extender-1 (E-1); homogenized together with glycerol, extender-2 (E-2). Bioxcell extender (E-3) used as control. Semen collected 3 times from endangered...

Multiple ovulation and embryo transfer (MOET) systems have been intensively implemented in Japanese Black cattle Japan to create herds out of these areas. Environmental conditions influence MOET efficiency. Thus, we describe results 137 vivo, non-surgical flushings performed between 2016-2020, a full-blood herd kept Spain the possible effects heat, year, bull, donor genetic value, metabolic condition. Additionally, 687 transfers were studied for conception rate (CR) recipient related...

We compared the effects of milk-feeding in 288 Holstein calves (72 per group) which were fed twice (2F) or thrice (3F) daily, with without addition hydrogenated fat-embedded calcium gluconate (G) supplemented starter food and daily diet up to age 9 months, on calves’ metabolism, growth, health, reproductive efficiency first pregnancy. The received 6 L milk replacer (130 g/L) had ad libitum access water textured calf gluconate. Gluconate supplementation promoted a “catch-up” growth their...