A5014291973- Cannabis and Cannabinoid Research
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- Tryptophan and brain disorders
- Diet, Metabolism, and Disease
- Sleep and Wakefulness Research
- Alcohol Consumption and Health Effects
- Pancreatic function and diabetes
- Neuroinflammation and Neurodegeneration Mechanisms
- Sphingolipid Metabolism and Signaling
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Neuroscience of respiration and sleep
- Regulation of Appetite and Obesity
- Neurogenesis and neuroplasticity mechanisms
- Stress Responses and Cortisol
- Diet and metabolism studies
- Substance Abuse Treatment and Outcomes
- Receptor Mechanisms and Signaling
- Forensic Toxicology and Drug Analysis
- Memory and Neural Mechanisms
- Prenatal Substance Exposure Effects
- Birth, Development, and Health
- Alzheimer's disease research and treatments
- Adipokines, Inflammation, and Metabolic Diseases
Hospital Regional Universitario de Málaga
2016-2025
Instituto de Investigación Biomédica de Málaga
2016-2025
Hospitais da Universidade de Coimbra
2018-2025
University of Coimbra
2025
Universidad de Málaga
2015-2024
Andalusian Centre for Nanomedicine and Biotechnology
2022-2024
Red de Investigación en Actividades Preventivas y Promoción de la Salud
2023-2024
European Monitoring Centre for Drugs and Drug Addiction
2024
Universidade Municipal de São Caetano do Sul
2024
Instituto de Salud Carlos III
2013-2023
Previous research has suggested a role for corticotropin-releasing factor (CRF) in the anxiogenic effects of stressful stimuli and ethanol withdrawal. This hypothesis was explored series experiments using intracranial microdialysis to monitor CRF-like immunoreactivity (CRF-IR) extracellular compartment rat amygdala. The synaptic origin CRF-IR release amygdala determined vitro by assessing Ca2+ dependency 4-aminopyridine stimulated from tissue preparations In vivo were performed awake rats...
Corticotropin-releasing factor (CRF) has been implicated in the mediation of stress-like and negative affective consequences withdrawal from drugs abuse, such as alcohol, cocaine, opiates. This study sought to determine whether brain CRF systems also have a role cannabinoid dependence. Rats were treated daily for 2 weeks with potent synthetic HU-210. Withdrawal, induced by antagonist SR 141716A, was accompanied marked elevation extracellular concentration distinct pattern Fos activation...
Recent studies suggest that the endocannabinoid system modulates feeding. Despite existence of central mechanisms for regulation food intake by endocannabinoids, evidence indicates peripheral may also exist. To test this hypothesis, we investigated (1) effects feeding on intestinal anandamide accumulation; (2) (intracerebroventricular) and (intraperitoneal) administration agonist anandamide, synthetic cannabinoid...
The long-term developmental and behavioral consequences of mammalian embryo culture are unknown. By altering the medium with addition FCS, we wanted to determine whether mouse embryos cultured under suboptimal conditions develop aberrant mRNA expression imprinting genes at blastocyst stage fetal development, growth, behavior adult mice affected. One-cell obtained from superovulated female B6CBAF 1 were for 4 days in K + -modified simplex optimized presence either 10% FCS or g/liter BSA....
Genetic and environmental factors produce different levels of DNA damage in spermatozoa. Usually, DNA-fragmented spermatozoa (DFS) are used with intracytoplasmic sperm injection (ICSI) treatments human reproduction, use DFS is still a matter concern. The purpose the present study was to investigate long-term consequences on development behavior mice generated by ICSI DFS. Using CD1 B6D2F1 mouse strains, oocytes were injected fresh or frozen-thawed without cryoprotector. This treatment...
The present study was designed to explore the relationship between cannabinoid and opioid receptors in animal models of opioid-induced reinforcement. acute administration SR141716A, a selective central CB1 receptor antagonist, blocked heroin self-administration rats, as well morphine-induced place preference morphine mice. Morphine-dependent animals injected with SR141716A exhibited partial opiate-like withdrawal syndrome that had limited consequences on operant responses for food induced...
ANIMAL models have revealed that psychoactive cannabinoids induce both anxiolytic and anxiety-like reactions which are dose- context-dependent. In the present study we examined acute actions of CB1 cannabinoid receptor antagonist SR 141716A in defensive withdrawal test elevated plus-maze rats. Acute administration (0.1, 1 3 mg kg−1) induced responses anxiety tests, at a dose kg−1. This had no effect on horizontal locomotor activity did not activate hypothalamus–pituitary–adrenal axis,...
THE present study demonstrates the presence of cannabinoid receptors in brain from early postnatal ages. Specific and saturable binding was observed forebrain remaining ages (2 5 days after birth). Female neonate exhibited a higher receptor density at 2 birth than males, but this trend inverted days. From day 10, could be measured more defined areas, i.e. striatum, limbic ventral mesencephalon. The ontogeny these three areas relatively similar, exhibiting progressive increase which maximised...
Abstract: This study was designed to determine the possible role of brain glucagon‐like peptide‐1 (GLP‐1) receptors in feeding behavior. In situ hybridization showed colocalization mRNAs for GLP‐1 receptors, glucokinase, and GLUT‐2 third ventricle wall adjacent arcuate nucleus, median eminence, supraoptic nucleus. These areas are considered contain glucose‐sensitive neurons mediating Because GLUT‐2, glucokinase proteins involved multistep process glucose sensing pancreatic β cells, specific...
We characterized the pharmacological properties of anandamide transport inhibitor N-(4-hydroxyphenyl)-arachidonamide (AM404) in rats and investigated effects this drug on behavioral responses associated with activation dopamine D(2) family receptors. Rat brain slices accumulated [(3)H]anandamide via a high-affinity mechanism that was blocked by AM404. When administered alone vivo, AM404 caused mild slow-developing hypokinesia significant 60 min after intracerebroventricular injection...