A5003912510- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- Reproductive System and Pregnancy
- Chromosomal and Genetic Variations
- COVID-19 Impact on Reproduction
- MicroRNA in disease regulation
- Animal Genetics and Reproduction
- Birth, Development, and Health
- Selenium in Biological Systems
- Ovarian function and disorders
- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- Preterm Birth and Chorioamnionitis
Tufts University
2020-2025
Centre National de la Recherche Scientifique
2015-2018
Clermont Université
2015-2018
Inserm
2015-2018
Université Clermont Auvergne
2018
Genetique Reproduction and Developpement
2018
Does a novel antioxidant formulation designed to restore redox balance within the male reproductive tract, reduce sperm DNA damage and increase pregnancy rates in mouse models of oxidative stress? Oral administration significantly reduced glutathione peroxidase 5 (GPX5), knockout mice restored near-normal levels subjected scrotal heat stress. Animal human studies have documented adverse effect on fertilization rates, embryo quality, miscarriage transfer de novo mutations offspring. Semen...
Sperm cells are remarkably complex and highly specialized compared to somatic cells. Their function is deliver the oocyte paternal genomic blueprint along with a pool of proteins RNAs so new generation can begin. Reproductive success, including optimal embryonic development healthy offspring, greatly depends on integrity sperm chromatin structure. It now well documented that DNA damage in linked reproductive failures both natural assisted conception (Assisted Technologies [ART]). This...
The transgenerational effects of exposing male mice to chronic social instability (CSI) stress are associated with decreased sperm levels multiple members the miR-34/449 family that persist after their mating through preimplantation embryo (PIE) development. Here we demonstrate importance these miRNA changes by showing restoring miR-34c in PIEs derived from CSI stressed males prevents elevated anxiety and defective sociability normally found specifically adult female offspring. It also...
The effects of chronically stressing male mice can be transmitted across generations by stress-specific changes in their sperm miRNA content, which induce phenotypes offspring. However, how each stress paradigm alters the levels distinct sets miRNAs is not known. We showed previously that exposure to chronic social instability (CSI) results elevated anxiety and reduced sociability specifically female offspring multiple because it reduces miR-34c stressed males unstressed Here, we describe...
The mammalian epididymis not only plays a fundamental role in the maturation of spermatozoa, but also provides protection against various stressors. foremost among these is threat posed by oxidative stress, which arises from an imbalance reactive oxygen species and can elicit damage to cellular lipids, proteins, nucleic acids. In mice, risk spermatozoa mitigated through expression secretion glutathione peroxidase 5 (GPX5) as major luminal scavenger proximal caput epididymidal segment....
Recent studies have revealed a well-defined higher order of chromosome architecture, named territories, in the human sperm nuclei. The purpose this work was, first, to investigate topology selected number chromosomes murine sperm; second, evaluate whether DNA damage has any consequence on architecture. Using fluorescence situ hybridization, confocal microscopy, and 3D-reconstruction approaches we demonstrate that positioning mouse nucleus is not random. Some tend occupy preferentially...
The effects of chronically stressing male mice can be transmitted across generations by stress-specific changes in their sperm miRNA content that induce phenotypes offspring. But how each stress paradigm alters the levels distinct sets miRNAs is not known. We showed previously exposure to chronic social instability (CSI) results elevated anxiety and reduced sociability specifically female offspring multiple because it reduces miR-34c stressed males unstressed Here we describe evidence
ABSTRACT The effects of chronically stressing male mice can be transmitted across generations by stress-specific changes in their sperm miRNA content that induce phenotypes offspring. But how each stress paradigm alters the levels distinct sets miRNAs is not understood. Here we describe evidence for a strocyte-derived exos omes ( A-Exos ) containing miR-34c mediating chronic social instability (CSI) suppesses sperm, which showed previously contributes to this protocol leads both elevated...