A5027864929- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Cholesterol and Lipid Metabolism
- Lipid metabolism and biosynthesis
- Drug Transport and Resistance Mechanisms
- Selenium in Biological Systems
- Hormonal and reproductive studies
- Reproductive System and Pregnancy
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ovarian function and disorders
- Neuroendocrine regulation and behavior
- Immunotherapy and Immune Responses
- Cancer, Lipids, and Metabolism
- Insect and Pesticide Research
- Effects and risks of endocrine disrupting chemicals
- Male Reproductive Health Studies
- Sexual Differentiation and Disorders
- Reproductive biology and impacts on aquatic species
- Reproductive Health and Technologies
- Lymphatic System and Diseases
- Circadian rhythm and melatonin
- Hypothalamic control of reproductive hormones
- Tryptophan and brain disorders
- Stress Responses and Cortisol
- Lymphatic Disorders and Treatments
Université Clermont Auvergne
2009-2024
Inserm
2012-2023
Centre National de la Recherche Scientifique
2012-2023
Genetique Reproduction and Developpement
2000-2023
Clermont Université
2011-2023
Institut Pascal
2013
Laboratoire d'Informatique, de Modélisation et d'Optimisation des Systèmes
2013
Saint Petersburg State Pediatric Medical University
2010
Tokushima University
2010
Université Laval
2003-2005
The mammalian epididymis provides sperm with an environment that promotes their maturation and protects them from external stresses. For example, it harbors array of antioxidants, including non-conventional glutathione peroxidase 5 (GPX5), to protect oxidative stress. To explore the role GPX5 in epididymis, we generated mice lack epididymal expression enzyme. Histological analyses Gpx5-/- epididymides cells revealed no obvious defects. Furthermore, there were apparent differences...
The epididymal epithelium secretes membranous vesicles, called epididymosomes, with which a complex mixture of proteins is associated. These vesicles transfer to spermatozoa selected involved in sperm maturation. Epididymosomes the human excurrent duct have been described, but their protein composition and possible functions are unknown. were collected during vasovasostomy procedures, purified submitted liquid chromatography hybrid quadrupole time-of-flight mass spectrometry. From all...
Prostasomes are human-specific lipid vesicles originating from the prostate and present in seminal plasma. They involved a number of biological functions such as sperm motility immunomodulation by The aim our study was to investigate whether prostasomes play role antioxidant status In cell suspensions obtained after elimination plasma centrifugation, reactive oxygen species (ROS) measured luminol-enhanced chemiluminescence were mainly produced polymorphonuclear neutrophils (PMN). addition...
During epididymal transit, mammalian spermatozoa acquire new proteins involved in the acquisition of motility and male gamete fertilising ability. We have previously shown that membranous vesicles called epididymosomes are transfer epididymal-originating to spermatozoa. The cytokine macrophage migration inhibitory factor (MIF) is one these but role played by MIF relation sperm maturation still remains unclear. As this protein has already been bear different functions depending on its...
Cholesterol is a key molecule in the mammalian physiology of especial particular importance for reproductive system as it common precursor steroid hormone synthesis. also recognized modulator sperm functions, not only at level gametogenesis. homeostasis regulation crucial posttesticular maturation, and imbalanced cholesterol levels may particularly affect these events. Metabolic lipid disorders (dyslipidemia) male fertility but are most time studied from angle endocrine/testicular...
Indoleamine 2,3-dioxygenase (IDO) is the first and rate-limiting enzyme of tryptophan catabolism through kynurenine pathway. Intriguingly, IDO constitutively highly expressed in mammalian epididymis contrast to most other tissues where induced by proinflammatory cytokines, such as interferons. To gain insight into role physiology epididymis, we studied both wild type Ido1(-/-)-deficient mice. In caput Ido1(-/-) animals, lack activity was not compensated tryptophan-catabolizing enzymes led...
We report here that spermatozoa of mice lacking both the sperm nucleus glutathione peroxidase 4 (snGPx4) and epididymal 5 (GPx5) activities display structural abnormalities including delayed defective nuclear compaction, instability DNA damage. show to counteract GPx activity losses, epididymis double KO animals mounted an antioxydant response resulting in a strong increase global H(2)O(2)-scavenger especially cauda epididymis. Quantitative RT-PCR data together with up-regulation scavengers...
This work shows that an overload of dietary cholesterol causes complete infertility in dyslipidemic male mice (the Liver X Receptor-deficient mouse model). Infertility resulted from post-testicular defects affecting the fertilizing potential spermatozoa. Spermatozoa cholesterol-fed lxr-/- animals were found to be dramatically less viable and motile, highly susceptible undergo a premature acrosome reaction. We also provide evidence, this lipid-induced is associated with accelerated appearance...
Abstract Testes produce spermatozoa that transit through and are stored in the epididymis where they acquire their fertilising capacities. Spermatozoa appear genital tract at puberty, long after immune system was trained to self-antigens. As a consequence, this organ has set strategies tolerate sperm antigens avoid autoimmune responses would specifically target destroy them. A recent study pointed Transforming Growth Factor-beta (TGF-β) signalling dendritic cells as crucial mechanism for...
Prostasomes are particular lipid vesicles secreted by the prostate in human semen and involved several physiological functions such as improvement of sperm motility or immunomodulation. We have previously shown that they reduced overall reactive oxygen species (ROS) production seminal polymorphonuclear neutrophils (PMN). The present study was conducted to define mechanism which prostasomes inhibit ROS blood PMN. luminol chemiluminescence measuring total PMN stimulated either a phorbol ester...
Mammalian spermatozoa undergo important plasma membrane maturation steps during epididymal transit. Among these, changes in lipids and cholesterol are of particular interest as they necessary for fertilization. However, molecular mechanisms regulating these transformations inside the epididymis still poorly understood. Liver X receptors (LXRs), nuclear oxysterols, major importance intracellular homeostasis, LXR−/−-deficient male mice have already been shown to reduced fertility at an age 5...
In this study we looked at the epididymides and spermatozoa of mice knocked-out for nuclear oxysterol receptors (LXR). We have shown that LXR-deficient exhibited upon ageing a severe disruption their caput associated with abnormal accumulation neutral lipids. The epididymis defaults were correlated sperm head fragility infertility. agreement observed defect in transgenic animals which both LXRα LXRβ isoforms disrupted, here are expressed cauda regions. was predominantly throughout mouse...
The epididymis maintains a state of immune tolerance towards spermatozoa while also protecting them and itself against infection acute inflammation. immunosuppressive enzyme indoleamine 2,3-dioxygenase 1 (Ido1) participates in this delicate local equilibrium. Using the mouse Ido1−/− model, we show here that absence IDO1 expression leads but not serum to (1) an increase inflammatory as evidenced by changes content cytokines chemokines, (2) engagement Th1-driven response Th17/Treg well Th1/Th2...