A5085885589- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Neuroinflammation and Neurodegeneration Mechanisms
- Amino Acid Enzymes and Metabolism
- Immune Response and Inflammation
- Memory and Neural Mechanisms
- Vitamin D Research Studies
- Neurogenesis and neuroplasticity mechanisms
- Lipid Membrane Structure and Behavior
- Ion channel regulation and function
- Dental Health and Care Utilization
- Oral microbiology and periodontitis research
- Cell Adhesion Molecules Research
- Antimicrobial Peptides and Activities
- Diet and metabolism studies
- Stress Responses and Cortisol
- Receptor Mechanisms and Signaling
- Neonatal and fetal brain pathology
- S100 Proteins and Annexins
- Mitochondrial Function and Pathology
- Ultrasound and Hyperthermia Applications
- Anesthesia and Neurotoxicity Research
- Reproductive Biology and Fertility
- Chronic Lymphocytic Leukemia Research
The University of Queensland
2015-2024
Women's Resource Center
2024
Park Centre for Mental Health
2016-2023
Washington University in St. Louis
2023
SUNY Downstate Health Sciences University
2022
Kings County Hospital Center
2022
State University of New York
2022
Creative Commons
2020
eHealth Initiative
2020
Cornell University
2020
The medial nucleus of the amygdala (MeA) plays a key role in innate emotional behaviors by relaying olfactory information to hypothalamic nuclei involved reproduction and defense. However, little is known about neuronal components this region or their olfactory-processing circuitry amygdala. Here, we have characterized neurons posteroventral division (MePV) using <i>GAD67-GFP</i> mouse. Based on electrophysiological properties GABA expression, unsupervised cluster analysis divided MePV into...
Abstract We studied the ability of recombinant human-active hemopoietic growth factors granulocyte-macrophage colony-stimulating factor (GM-CSFrh) and granulocyte (G-CSFrh) to activate receptor-mediated transduction pathways which have been implicated in stimulation cytotoxic functions granulocytes. With use a panel fluorescent probes, we found that these two exerted no detectable immediate effect on resting transmembrane electrical potential, intracellular concentration free calcium ions,...
The epididymal epithelium secretes membranous vesicles, called epididymosomes, with which a complex mixture of proteins is associated. These vesicles transfer to spermatozoa selected involved in sperm maturation. Epididymosomes the human excurrent duct have been described, but their protein composition and possible functions are unknown. were collected during vasovasostomy procedures, purified submitted liquid chromatography hybrid quadrupole time-of-flight mass spectrometry. From all...
In Alzheimer disease and related disorders, the microtubule-associated protein tau aggregates forms cytoplasmic lesions that impair neuronal physiology at many levels. addition to affecting host neuron, also spread neighboring, recipient cells where misfolded aggregates, in a manner similar prions, actively corrupt proper folding of soluble tau, thereby cellular functions. One vehicle for transmission are secretory nanovesicles known as exosomes. Here, we established simple model circuit...
It is now widely accepted that hippocampal neurogenesis underpins critical cognitive functions, such as learning and memory. To assess the behavioral importance of adult-born neurons, we developed a novel knock-in mouse model allowed us to specifically reversibly ablate neurons at an immature stage. In these mice, diphtheria toxin receptor (DTR) expressed under control doublecortin (DCX) promoter, which allows for specific ablation DCX-expressing after administration while leaving neural...
The basolateral amygdala (BLA) and prefrontal cortex (PFC) are partners in fear learning extinction. Intercalated (ITC) cells inhibitory neurons that surround the BLA. Lateral ITC (lITC) provide feed-forward inhibition to BLA principal neurons, whereas medial (mITC) form an interface between central (CeA). Notably, infralimbic (IL) input mITC is thought play a key role Here, using targeted optogenetic stimulation, we show lITC receive auditory from cortical thalamic regions. IL inputs...
The reduced pathogenicity of the omicron BA.1 sub-lineage compared to earlier variants is well described, although whether such attenuation retained for later like BA.5 and XBB remains controversial. We show that isolates were significantly more pathogenic in K18-hACE2 mice than a isolate, showing increased neurotropic potential, resulting fulminant brain infection mortality, similar seen original ancestral isolates. also infected human cortical organoids greater extent In brains mice,...
Fertility of frozen-thawed bull sperm is reduced by cryopreservation. Freezing-thawing procedures can result in as much a sevenfold fertility decrease. Sperm mortality and loss motility do not fully explain the cryopreserved semen; they may be partially explained surface proteins, which are necessary for fertilization. We have previously identified P25b, protein, associated with index bulls used artificial insemination. Using Western blotting techniques, we evaluated P25b levels before after...
Abstract D ‐Serine is a co‐agonist at the NMDA receptor glycine‐binding site. Early studies have emphasized glial localization for ‐serine. However nature of cells has not been fully resolved, because previous ‐serine antibodies needed glutaraldehyde‐fixation, precluding co‐localization with fixation‐sensitive antigens. We raised new antibody optimized formaldehyde‐fixation. Light and electron microscopic observations indicated that was concentrated into vesicle‐like compartments in...
Clostridium difficile, a common enteric pathogen, mediates tissue damage and intestinal fluid secretion by release of two protein exotoxins: toxin A, an enterotoxin, B, cytotoxin. Because A elicits intense inflammatory reaction in vivo, we studied the effects highly purified C. difficile toxins on activation human granulocytes. Toxin at concentrations 10(-7) to 10(-6) M, but not elicited significant chemotactic chemokinetic response granulocytes that was comparable with induced factor N-FMLP...
It is well established that the coordinated regulation of activity-dependent gene expression by histone acetyltransferase (HAT) family transcriptional coactivators crucial for formation contextual fear and spatial memory, hippocampal synaptic plasticity. However, no studies have examined role this epigenetic mechanism within infralimbic prefrontal cortex (ILPFC), an area brain essential consolidation extinction memory. Here we report a postextinction training infusion combined p300/CBP...
New neurons are continually generated from the resident populations of precursor cells in selective niches adult mammalian brain such as hippocampal dentate gyrus and olfactory bulb. However, whether present amygdala, their neurogenic capacity, is not known. Using neurosphere assay, we demonstrate that a small number cells, majority which express Achaete-scute complex homolog 1 (Ascl1), basolateral amygdala (BLA) mouse. neuron-specific Thy1-YFP transgenic mice, show YFP+ BLA-derived...
The medial amygdala (MeA) is a central hub in the olfactory neural network. It receives vomeronasal information directly from accessory bulb (AOB) and main largely via odor-processing regions such as cortical (CoA). How these inputs are processed by MeA neurons poorly understood. Using GAD67-GFP mouse, we show that principal receive convergent AOB CoA inputs. Somatically recorded synaptic had slower kinetics than inputs, suggesting they electrotonically more distant. Field potential...
Deep-sea fishes evolved an unconventional visual system to optimize vision in twilight conditions.
Abstract Vitamin D deficiency may exacerbate adverse neurocognitive outcomes in the progression of diseases such as Parkinson's, Alzheimer's, and other dementias. Mild cognitive impairment (MCI) is prodromal for these disorders neuroimaging studies suggest that, elderly, this associated with a reduction hippocampal volume white matter structural integrity. To test whether vitamin neuroanatomical correlates MCI, we analyzed an existing diffusion MRI dataset elderly patients MCI. Based on...
The blood-brain barrier (BBB) is a dynamic diffusional regulating the molecular and chemical flux between blood brain, thereby preserving cerebral homeostasis. Endothelial cells form core anatomical component of BBB based on properties such as specialized junctional complexes cells, which restricts paracellular transport, extremely low levels vesicular restricting transcytosis. In performing its protective function, also constrains entry therapeutics into hampering treatment various...
Abstract Advanced physiological aging is associated with impaired cognitive performance and the inability to induce long-term potentiation (LTP), an electrophysiological correlate of memory. Here, we demonstrate in physiologically aged, senescent mouse brain that scanning ultrasound combined microbubbles (SUS +MB ), by transiently opening blood–brain barrier, fully restores LTP induction dentate gyrus hippocampus. Intriguingly, SUS treatment without only i.e., uptake blood-borne factors,...
Abstract Polyunsaturated free fatty acids (FFAs) such as arachidonic acid, released by phospholipase activity on membrane phospholipids, have long been considered beneficial for learning and memory are known modulators of neurotransmission synaptic plasticity. However, the precise nature other FFA phospholipid changes in specific areas brain during is unknown. Here, using a targeted lipidomics approach to characterise FFAs phospholipids across rat brain, we demonstrated that highest...
Abstract It is generally assumed that rodent brains can be used as representative models of neurochemical function in other species, such humans. We have compared the distributions predominant glial glutamate transporters rodents, rabbits, cats, pigs, monkeys, and identify similarities but also significant differences between species. GLT‐1v, which abundantly expressed by astrocytes, only a rare subset astrocytes cats humans, appears to absent from rabbits monkeys. Conversely, pig brain...
purpose. To determine whether structural plasticity is evident in human retinal tissues response to age-related macular degeneration (AMD). Remodeling events such as sprouting of neuronal processes and the reconnection synapses are essential elements repairing any damage adult nervous might occur insults strokes or AMD. methods. The anatomic architecture normal AMD-affected retinas was examined central, midperipheral, far-peripheral regions. retina, by virtue its well-organized laminar...
<i>Background/Aims:</i><i>Streptococcus mutans</i>, the major etiological agent of dental caries, has a measurable impact on domestic and global health care costs. Though persistent in oral cavity despite conventional hygiene, <i>S. mutans</i> can be excluded from intact biofilms through competitive exclusion by other microorganisms. This suggests that therapies capable selectively eliminating while limiting damage to normal flora might effective long-term...