A5063976363- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurobiology and Insect Physiology Research
- Tryptophan and brain disorders
- Memory and Neural Mechanisms
- Axon Guidance and Neuronal Signaling
- Stress Responses and Cortisol
- Epigenetics and DNA Methylation
- Nerve injury and regeneration
- Olfactory and Sensory Function Studies
- RNA modifications and cancer
- Developmental Biology and Gene Regulation
- Vitamin D Research Studies
- Anesthesia and Neurotoxicity Research
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Hormonal Regulation and Hypertension
- RNA Interference and Gene Delivery
- Growth Hormone and Insulin-like Growth Factors
- Animal testing and alternatives
- Invertebrate Immune Response Mechanisms
The University of Queensland
2014-2024
Mater Research
2017-2024
Translational Research Institute
2021-2023
Queensland Eye Institute
2014-2017
Tata Institute of Fundamental Research
2000-2005
Recent advances in next-generation sequencing technology allow high-throughput cDNA (RNA-Seq) to be widely applied transcriptomic studies, particular for detecting differentially expressed genes between groups. Many software packages have been developed the identification of (DEGs) treatment groups based on RNA-Seq data. However, there is a lack consensus how approach an optimal study design and choice suitable analysis. In this comparative we evaluate performance three most frequently used...
Adult hippocampal neurogenesis is a critical form of cellular plasticity that greatly influenced by neural activity. Among the neurotransmitters are widely implicated in regulating this process serotonin and norepinephrine, levels which modulated stress, depression clinical antidepressants. However, studies to date have failed address direct role for either neurotransmitter precursor Here we show norepinephrine but not directly activates self-renewing multipotent precursors, including stem...
Abstract Retrotransposons are mobile DNA sequences duplicated via transcription and reverse of an RNA intermediate. Cis -regulatory elements encoded by retrotransposons can also promote the adjacent genes. Somatic LINE-1 (L1) retrotransposon insertions have been detected in mammalian neurons. It is, however, unclear whether L1 only some neuronal lineages or therein neurodevelopmental gene expression. Here we report programmed activation SOX6, a factor critical for parvalbumin (PV)...
Slow-onset adaptive changes that arise from sustained antidepressant treatment, such as enhanced adult hippocampal neurogenesis and increased trophic factor expression, play a key role in the behavioral effects of antidepressants. α 2 -Adrenoceptors contribute to modulation mood are potential targets for development faster acting We investigated influence -adrenoceptors on neurogenesis. Our results indicate -adrenoceptor agonists, clonidine guanabenz, decrease through selective effect...
Abstract Within the two neurogenic niches of adult mammalian brain, i.e., subventricular zone lining lateral ventricle and subgranular hippocampus, there exist distinct populations proliferating neural precursor cells that differentiate to generate new neurons. Numerous studies have suggested epigenetic regulation by histone‐modifying proteins is important in guiding differentiation during development; however, role these regulating activity remains poorly understood. Here we examine an NAD...
New neurons are continually generated from the resident populations of precursor cells in selective niches adult mammalian brain such as hippocampal dentate gyrus and olfactory bulb. However, whether present amygdala, their neurogenic capacity, is not known. Using neurosphere assay, we demonstrate that a small number cells, majority which express Achaete-scute complex homolog 1 (Ascl1), basolateral amygdala (BLA) mouse. neuron-specific Thy1-YFP transgenic mice, show YFP+ BLA-derived...
Activity-dependent changes in the number of AMPA-type glutamate receptors (AMPARs) at synapse underpin expression LTP and LTD, cellular correlates learning memory. Post-translational ubiquitination has emerged as a key regulator trafficking surface AMPARs, with GluA1 subunit Lys-868 controlling post-endocytic sorting into late endosome for degradation, thereby regulating their stability synapses. However, physiological significance remains unknown. In this study, we generated mice knock-in...
The activity of neural precursor cells in the adult hippocampus is regulated by various stimuli; however, whether these stimuli regulate same or different populations remains unknown. Here, we developed a novel cell-sorting protocol that allows purification to homogeneity neurosphere-forming precursors from mouse and examined responsiveness individual using clonal assay. We show within Hes5-GFP + /Nestin-GFP /EGFR cell population, which comprises majority precursors, there are two distinct...
Norepinephrine regulates latent neural stem cell activity and adult hippocampal neurogenesis, has an important role in modulating functions such as learning, memory mood. Adult neurogenesis is a multi-stage process, spanning from the activation proliferation of cells, to their differentiation into neurons. However, stage-specific effects noradrenergic receptors regulating remain poorly understood. In this study, we used transgenic Nestin-GFP mice neurosphere assays show that modulation α2-...
Abstract Recent advances in next-generation sequencing technology allow high-throughput cDNA (RNA-Seq) to be widely applied transcriptomic studies, particular for detecting differentially expressed genes between groups. Many software packages have been developed the identification of (DEGs) treatment groups based on RNA-Seq data. However, there is a lack consensus how approach an optimal study design and choice suitable analysis. In this comparative we evaluate performance three most...
Olfactory receptor neurons and the interneurons of olfactory lobe are organized in distinct units called glomeruli. We have used expression patterns genetic analysis to demonstrate that a combinatorial code Roundabout(Robo) receptors act position sensory terminals within lobe. Groups possess blends Robo Robo3 disruption levels by loss-of-function or ectopic results aberrant targeting. In wild type, most send collateral branches contralateral Our data suggests guidance axons across brain...
Hippocampal function is critical for spatial and contextual learning, its decline with age contributes to cognitive impairment. Exercise can improve hippocampal function, however, the amount of exercise mechanisms mediating improvement remain largely unknown. Here, we show reverses learning deficits in aged (24 months) female mice but only when it occurs a specific duration, longer or shorter periods proving ineffective. A spike levels growth hormone (GH) corresponding increase neurogenesis...
While it is now well-established that resident populations of stem and progenitor cells drive neurogenesis in the adult brain, a growing body evidence indicates these new neurons play pivotal role spatial learning, memory, mood regulation. As such, interest gathering to develop strategies harness brain's endogenous reservoir cells, with view newborn may help overcome loss neural cognitive function occurs during neurodegenerative disease psychiatric illness. Here we review for presence cell...
The first centers for processing of odor information by animals lie in the olfactory lobe. Sensory neurons from periphery synapse with interneurons anatomically recognizable units, termed glomeruli, seen both insects and vertebrates. mechanisms that underlie formation functional maps odor-world glomeruli within lobe remains unclear. We address basis sensory targeting fruitfly Drosophila show one class neurons, those Atonal lineage, plays a crucial role glomerular patterning. Atonal-dependent...
The regulation of neural precursor cell (NPC) activity is the major determinant rate neuronal production in neurogenic regions adult brain. Here, we show that Oncostatin M (Osm) and its receptor, OsmRβ, are both expressed subventricular zone (SVZ) contradistinction to leukemia inhibitory factor ciliary neutrophic factor, Osm directly inhibits proliferation NPCs as measured by a decreased level neurosphere formation vitro. Similarly, intraventricular infusion dramatically decreases pool SVZ...
The hippocampal dentate gyrus (DG) is a major region of the adult rodent brain in which neurogenesis occurs throughout life. EphA4 receptor, regulates and boundary formation developing brain, also expressed DG, but whether it not known. Here, we show that, mouse inhibits precursor cell proliferation does affect differentiation or survival. Genetic deletion pharmacological inhibition significantly increased vivo vitro, by blocking forward signaling. was mature DG neurons neural cells, an...
Abstract Background The development of nervous systems involves reciprocal interactions between neurons and glia. In the Drosophila olfactory system, peripheral glial cells arise from sensory lineages specified by basic helix-loop-helix transcription factor, Atonal. These glia wrap around developing axons early during pattern three distinct fascicles as they exit antenna. moth Manduca sexta , an additional set central migrate to base antennal nerve where sort their glomerular targets. this...
Enhanced adult hippocampal neurogenesis and increased trophic factor expression mediate the behavioral effects of antidepressants. We investigated influence alpha2‐adrenoceptors on neurogenesis. found that alpha2 agonists selectively decrease progenitor proliferation. These persist in Dbh−/− mice lacking norepinephrine, suggesting an action through alpha2‐heteroceptors. Adult progenitors express all alpha2‐adrenoceptor subtypes, decreased neurosphere frequency BrdU incorporation indicate...