A5077737272- Single-cell and spatial transcriptomics
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Pluripotent Stem Cells Research
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cell Image Analysis Techniques
- Glioma Diagnosis and Treatment
- Mesenchymal stem cell research
- Cancer Cells and Metastasis
- Nuclear Receptors and Signaling
- Glycosylation and Glycoproteins Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- CRISPR and Genetic Engineering
- Microtubule and mitosis dynamics
- Neuroscience and Neuropharmacology Research
- Monoclonal and Polyclonal Antibodies Research
- Transplantation: Methods and Outcomes
- Calcium signaling and nucleotide metabolism
- Neutropenia and Cancer Infections
- Micro and Nano Robotics
- Caveolin-1 and cellular processes
- Hedgehog Signaling Pathway Studies
- 3D Printing in Biomedical Research
- Phagocytosis and Immune Regulation
The University of Queensland
2010-2019
Monash University
2011-2012
Australian National University
2000
Individual tumour cells display diverse functional behaviours in terms of proliferation rate, cell–cell interactions, metastatic potential and sensitivity to therapy. Moreover, sequencing studies have demonstrated surprising levels genetic diversity between individual patient tumours the same type. Tumour heterogeneity presents a significant therapeutic challenge as cell types within can respond differently therapies, inter-patient may prevent development general treatments for cancer. One...
Abstract Inflammasomes are large protein complexes induced by a wide range of microbial, stress, and environmental stimuli that function to induce cell death inflammatory cytokine processing. Formation an inflammasome involves dramatic relocalization the adapter apoptosis-associated speck-like containing caspase recruitment domain (ASC) into single speck. We have developed flow cytometric assay for formation, time flight evaluation, which detects change in ASC distribution within cell. The...
Abstract A critical factor in the successful isolation of new antibodies by phage display is presentation a correctly folded antigen. While this relatively simple for soluble proteins which can be purified and immobilized onto plastic surface, membrane offer significant challenges antibody discovery. Whole cell panning allows protein its native conformation, but complicated low target antigen density, high background irrelevant antigens non-specific binding particles to surfaces. The method...
Brain cancer research has been hampered by a paucity of viable clinical tissue sufficient quality and quantity for experimental research. This driven researchers to rely heavily on long term cultured cells which no longer represent the cancers from they were derived. Resection brain tumors, particularly at interface between normal tumorigenic tissue, can be carried out using an ultrasonic surgical aspirator (CUSA) that deposits liquid (blood irrigation fluid) resected into sterile bottle...
Large-scale proliferation and multi-lineage differentiation capabilities make neural stem cells (NSCs) a promising renewable source of for therapeutic applications. However, the practical application neuronal cell replacement is limited by heterogeneity NSC progeny, relatively low yield neurons, predominance astrocytes, poor survival donor following transplantation potential uncontrolled precursor cells. To address these impediments, we have developed method generation highly enriched...
The activity of neural precursor cells in the adult hippocampus is regulated by various stimuli; however, whether these stimuli regulate same or different populations remains unknown. Here, we developed a novel cell-sorting protocol that allows purification to homogeneity neurosphere-forming precursors from mouse and examined responsiveness individual using clonal assay. We show within Hes5-GFP + /Nestin-GFP /EGFR cell population, which comprises majority precursors, there are two distinct...
Human embryonic stem cell (hESC) derivatives show promise as viable therapy options for multiple disorders in different tissues. Recent advances biology have lead to the reliable production and detailed molecular characterisation of a range cell-types. However, role mitochondria during differentiation has yet be fully elucidated. Mitochondria mediate cells response altered energy requirements (e.g. cardiomyocyte contraction) and, such, mitochondrial phenotype is likely change dynamic process...
Abstract Flow cytometry is an important drug discovery tool because it permits high‐content multiparameter analysis of individual cells. A new method dramatically enhanced screening throughput by multiplexing many discrete fixed cell populations; however, this not suited to assays requiring functional cellular responses. HEK293 cells were transfected with unique mutant glycine receptors. Mutant receptor expression was confirmed coexpression yellow fluorescent protein (YFP). Commercially...
Abstract Absolute neutrophil count (ANC) is used clinically to monitor physiological dysfunctions such as myelosuppression or infection. In the research laboratory, ANC a valuable measure evolution of wide range disease states in models. Flow cytometry (FCM) fast, widely approach confidently identify thousands cells within minutes. FCM can be optimised for absolute counting using spiked‐in beads by measuring sample volume analysed. Here we combine 1A8 antibody, specific mouse granulocyte...
Abstract Cell sorting flow cytometers sort cells by applying electrical charges to a stream that forms liquid droplets containing the at set time after sample interrogation. The correct apply these is determined based on calibration beads and automated technology. central tenet of this method accurately indicate yield sorted using same instrument parameters. HEK293T were incubated with Accudrop™ beads. incorporation phagocytosis was confirmed imaging cytometry. Cells analyzed unmodified...
Abstract Flow cytometry based electrostatic cell sorting is an important tool in the separation of populations. Existing instruments can sort single cells into multi‐well collection plates, and keep track origin sorted well location. However currently results reflect population distribution fail to capture diversity. Software was designed that implements a novel approach, “Slice Dice Sorting,” links graphical representation plate logic ensures are sampled from all areas defined by region/s....
Use of flow cytometry to detect pluripotency markers on or in human embryonic stem cells (hESCs) is a powerful analytical tool. However, current staining methodologies for high-content analysis large numbers samples utilize quantities primary and secondary antibodies, are time consuming, may suffer from sample-to-sample variability. To circumvent these issues, we have developed reproducible, quick, cost-effective method 12 populations hESCs grown under different conditions by labeling each...
Antibodies are routinely used to study the activity of transcription factors, using various in vitro and vivo approaches such as electrophoretic mobility shift assay, enzyme-linked immunosorbent genome-wide method analysis coupled with next generation sequencing, or mass spectrometry. More recently, a new application for antibodies has emerged crystallisation scaffolds difficult crystallise proteins, factors. Only few rare cases, have been modulate there is real gap our knowledge on how...
Neurogenesis occurs continuously in two brain regions of adult mammals, underpinned by a pool resident neural stem cells (NSCs) that can differentiate into all cell types. To advance our understanding NSC function and to develop therapeutic diagnostic approaches, it is important accurately identify enrich for NSCs. There are no definitive markers the identification enrichment NSCs present mouse brain. Recently, fluorescent rosamine dye, CDy1, has been identified as label pluripotency...
High morbidity and mortality are common traits of malignant tumours identification the cells responsible is a focus on-going research. Many studies now reporting use antibodies specific to Clusters Differentiation (CD) cell surface antigens identify tumour-initiating (TIC) populations in neural tumours. Medulloblastoma one most brain children despite considerable amount research investigating this tumour, identity TICs, means by which such can be targeted remain largely unknown. Current...