A5025449322- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Cholinesterase and Neurodegenerative Diseases
- Extracellular vesicles in disease
- Hereditary Neurological Disorders
- Autophagy in Disease and Therapy
- RNA Interference and Gene Delivery
- Endoplasmic Reticulum Stress and Disease
- Galectins and Cancer Biology
- Botulinum Toxin and Related Neurological Disorders
- Wnt/β-catenin signaling in development and cancer
- Lipid Membrane Structure and Behavior
- Hypothalamic control of reproductive hormones
- Curcumin's Biomedical Applications
- Coffee research and impacts
- Advanced Nanomaterials in Catalysis
- Plant responses to water stress
- Calcium signaling and nucleotide metabolism
- Signaling Pathways in Disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Dementia and Cognitive Impairment Research
- Aluminum toxicity and tolerance in plants and animals
- Metabolism, Diabetes, and Cancer
- Tea Polyphenols and Effects
- Cancer Immunotherapy and Biomarkers
Huazhong University of Science and Technology
2018-2024
Zhejiang Provincial People's Hospital
2024
Hangzhou Normal University
2024
Peking University
2024
Peking University Third Hospital
2024
Zhejiang University
2024
Northeast Forestry University
2023
Park Centre for Mental Health
2015-2018
The University of Queensland
2015-2018
Wuhan University
2010-2013
In Alzheimer disease and related disorders, the microtubule-associated protein tau aggregates forms cytoplasmic lesions that impair neuronal physiology at many levels. addition to affecting host neuron, also spread neighboring, recipient cells where misfolded aggregates, in a manner similar prions, actively corrupt proper folding of soluble tau, thereby cellular functions. One vehicle for transmission are secretory nanovesicles known as exosomes. Here, we established simple model circuit...
Global epidemic studies have suggested that coffee consumption is reversely correlated with the incidence of type 2 diabetes mellitus (T2DM), a metabolic disease. The misfolding human islet amyloid polypeptide (hIAPP) regarded as one causative factors T2DM. Coffee extracts three major active components: caffeine, caffeic acid (CA), and chlorogenic (CGA). In this study, effects these components, well dihydrocaffeic (DHCA) (a metabolite CGA CA), on amyloidogenicity hIAPP were investigated by...
Abstract The microtubule-associated protein tau has a critical role in Alzheimer’s disease and other tauopathies. A proposed pathomechanism the progression of tauopathies is trans-synaptic spreading seeds, with for exosomes which are secretory nanovesicles generated by late endosomes. Our previous work demonstrated that brain-derived isolated from transgenic rTg4510 mice encapsulate seeds ability to induce aggregation recipient cells. We had also shown can hijack endosomal pathway spread...
Abstract Endonuclease G (ENDOG), a mitochondrial nuclease, is known to participate in many cellular processes, including apoptosis and paternal elimination, while its role autophagy remains unclear. Here, we report that ENDOG released from mitochondria promotes during starvation, which find be evolutionally conserved across species by performing experiments human cell lines, mice, Drosophila C. elegans . Under Glycogen synthase kinase 3 beta-mediated phosphorylation of at Thr-128 Ser-288...
Efficient therapeutic strategies that concurrently target both Aβ aggregation and oxidative stress in the Alzheimer's disease (AD) microenvironment emerge as a cutting-edge tool to combat intricate pathogenesis of AD. Here, multivalent nanobody conjugate with rigid, reactive oxygen species (ROS) scavenging scaffold is developed achieve simultaneous amyloidogenesis mitigation, ROS elimination, plaque clearance. Grafting segment (33-GLMVGGVVIA-42) into third complementary-determining region...
Background Neuron loss, glial activation and vascular degeneration are common sequelae of ischemia-reperfusion (I/R) injury in ocular diseases. The present study was conducted to explore the ability curcumin inhibit retinal I/R injury, investigate underlying mechanisms drug effects. Methodology/Principal Findings Different dosages were administered. induced by elevating intraocular pressure for 60 min followed reperfusion. Cell bodies, brn3a stained cells TUNEL positive apoptotic ganglion...
Alzheimer's disease is characterized by the accumulation of amyloid-β (Aβ) and Tau in brain. In mature neurons, concentrated axon found at lower levels dendrite where it required for targeting Fyn to spines. Here mediates Aβ toxicity, which vastly abrogated when either deleted or a truncated form (Tau(1-255)) co-expressed. Interestingly, MAP2, microtubule-binding protein with mainly dendritic localization that shares Fyn-binding motifs Tau, does not mediate Aβ's synaptic toxicity absence...
Endoplasmic reticulum (ER) stress has been demonstrated to contribute neurodegeneration in multiple ocular diseases. However, whether ER can induce vascular degeneration the retina remains unknown. We investigated possible role of retinal vivo, and effects resveratrol on tunicamycin ischemia reperfusion (I/R)-induced degeneration.Different dosages tunicamycin, an inducer, were injected into vitreous mouse eyes. Retinal I/R injury was induced by elevating intraocular pressure for 60 minutes...
Bisphenol A (BPA) is a chemical compound widely used in manufacturing plastic products. Recent epidemiological studies suggest BPA exposure positively associated with the incidence of type 2 diabetes mellitus (T2DM), however mechanisms underlying this link remain unclear. Human islet amyloid polypeptide (hIAPP) hormone synthesized and secreted by pancreatic β-cells. Misfolding hIAPP into toxic oligomers mature fibrils can disrupt cell membrane lead to β-cell death, which regarded as one...
Hepatocellular carcinoma (HCC) is one of the most prevalent cancers worldwide and accounts for more than 90% primary liver cancer. The advent immune checkpoint inhibitor (ICI)-related therapies combined with angiogenesis inhibition has revolutionized treatment HCC in late-stage unresectable HCC, as ICIs alone were disappointing treating HCC. In addition to altered microenvironment, abnormal lipid metabolism been extensively characterized various types Stains are known their...
The protein tyrosine kinase Pyk2 is encoded by PTK2B, a novel Alzheimer’s disease (AD) susceptibility variant, with the PTK2B risk allele being associated increased mRNA levels, suggestive of levels. However, role Pyk2, member focal adhesion (FAK) family, in AD pathology and its regulation are largely unknown. To address this, we generated mice neuronal expression human Pyk2. Because had previously reported an association hyperphosphorylated tau (a hallmark feature AD) transgenic pR5 mice,...
Glycogen is a highly branched glucose polymer functioning as buffer in animals. Multiple-detector size exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis were used to examine the structure of undegraded native liver glycogen (both whole enzymatically debranched) function molecular size, isolated from livers healthy db/db mice (the latter type 2 diabetic model). Both fully debranched levels showed fundamental differences between mice. Healthy had greater population...
Of 10 variation sites between sequences of amyloid-resistant porcine islet amyloid polypeptide (pIAPP) and amyloid-prone human IAPP (hIAPP), seven locate within residues 17-29, the most amyloidogenic fragment hIAPP. To investigate how these variations affect amyloidogenicity, 26 IAPP(17-29) or IAPP(20-29) variants were synthesized their secondary structures, oligomerization cytotoxicity studied. Our results indicated that pIAPP fragments are refractory to formation significantly less...
Abstract Background Glioma stem cells (GSCs) are the root cause of relapse and treatment resistance in glioblastoma (GBM). In GSCs, hypoxia microenvironment is known to facilitate maintenance cells, evolutionally conserved autophagy regulates cell homeostasis control population. The precise involvement regulation hypoxic conditions maintaining stemness GSCs remains unclear. Methods association was first assessed by silico analysis validation vitro. databases clinical specimens were used...
Abstract DNA methylation can serve as proxies of (past) exposures and disease progression, well provide clues to reveal the pathogenesis amyotrophic lateral sclerosis (ALS) from perspective gene-environment interactions. Here, we conducted an epigenome-wide analysis in peripheral blood cells 480 cases ALS (203 had survival data) 207 health controls Han Chinese ancestry (Peking University Third Hospital cohort, PUTH-ALS), whose genomic patterns were quantified using Illumina Infinium Human...
Background Mutations in the presenilin-1 ( PSEN1 ), presenilin-2 PSEN2 and amyloid precursor protein APP ) genes have been commonly identified early-onset Alzheimer's disease (EOAD). Some of mutations three causative genes, especially gene, result variable phenotypes exhibit clinical heterogeneity among EOAD families. Methods Using next-generation sequencing (NGS), we performed genetic screening a Chinese cohort 18 patients with EOAD, consisting five familial 13 sporadic cases. Results We...