A5100343769- Cancer-related molecular mechanisms research
- Microbial Metabolic Engineering and Bioproduction
- X-ray Diffraction in Crystallography
- Advanced biosensing and bioanalysis techniques
- Crystallization and Solubility Studies
- Ubiquitin and proteasome pathways
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- DNA and Nucleic Acid Chemistry
- Cancer, Hypoxia, and Metabolism
- Natural product bioactivities and synthesis
- Enzyme Catalysis and Immobilization
- Cancer, Lipids, and Metabolism
- Ferroptosis and cancer prognosis
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- RNA Research and Splicing
- Metabolomics and Mass Spectrometry Studies
- Cholesterol and Lipid Metabolism
- Lipoproteins and Cardiovascular Health
- Circular RNAs in diseases
- Skin and Cellular Biology Research
- Dermatological and Skeletal Disorders
Shanghai University
2025
Shanghai University of Traditional Chinese Medicine
2025
California Environmental Protection Agency
2021-2025
Heze University
2025
Beijing Botanical Garden
2020-2025
Xuzhou Medical College
2022-2025
Tsinghua University
2018-2024
Tianjin University of Traditional Chinese Medicine
2023-2024
University of Science and Technology of China
2023-2024
Jilin University
2016-2024
UV−vis and CD spectroscopy reveal that a tricationic cyanine dye spontaneously assembles into helical J-aggregate in the presence of double-helical DNA template. The stability is strongly dependent on concentration length manner reflects high degree cooperativity formation aggregate. Slight changes environmental conditions such as temperature ionic strength result interconversion between J- H-aggregates. aggregate likely consists dimeric units assembled an offset, face-to-face orientation...
3,3'-Diethylthiadicarbocyanine (DiSC2(5)) is a symmetrical cationic cyanine dye consisting of two N-ethylated benzothiazole groups linked by pentamethine bridge. Spectroscopic analysis indicates dimerization the in presence duplex DNA sequences alternating adenine/thymine (A/T) or inosine/cytosine (I/C) residues, based on following observations: (i) absorption maximum shifts from 647 to 590 nm, (ii) exciton splitting observed induced circular dichroism spectrum, and (iii) fluorescence...
Incorporation of a guanidine functional group into the PNA backbone facilitates cellular uptake mammalian cells with efficiency comparable to that TAT transduction domain. The modified recognizes and binds complementary DNA strand in accordance Watson−Crick recognition rules. However, unlike polypyrimidine which 2:1 stoichiometry, 1:1 ratio form highly stable duplex.
Abstract Endonuclease G (ENDOG), a mitochondrial nuclease, is known to participate in many cellular processes, including apoptosis and paternal elimination, while its role autophagy remains unclear. Here, we report that ENDOG released from mitochondria promotes during starvation, which find be evolutionally conserved across species by performing experiments human cell lines, mice, Drosophila C. elegans . Under Glycogen synthase kinase 3 beta-mediated phosphorylation of at Thr-128 Ser-288...
Quantum dots are a new class of fluorophores, whose more prominent characteristics include size-tunable, narrow, fluorescence emission bands and broad overlapping excitation spectra multiple color dots. Here, we present an efficient, versatile, gentle approach for intracellular delivery quantum that is easily extended to multicolor optical coding mammalian cells. In this method, nine residue biotinylated l-arginine peptide used enhance streptavidin conjugated into
The incidence of bladder urothelial carcinoma (UC), a common malignancy the urinary tract, is approximately three times higher in men than women. High expression mitotic kinase BUB1 associated with occurrence and development several cancers, although relationship between tumorigenesis remains unclear.Using microarray approach, we found increased human BCa. association STAT3 phosphorylation was determined through molecular cell biological methods. We evaluated impact pharmacologic inhibition...
Recent evidence suggests that metabolic shutdown alone does not fully explain how bacteria exhibit phenotypic antibiotic tolerance. In an attempt to investigate the range of starvation-induced physiological responses underlying tolerance development, we found active maintenance transmembrane proton motive force (PMF) is essential for prolonged expression in bacteria. Eradication tolerant sub-population could be achieved by disruption PMF using ionophore CCCP, or through suppression...
Parathyroid hormone (PTH) and its related peptide (PTH-related 1–34) are two of the Food Drug Administration-approved bone-promoting drugs for age-related osteoporosis. Treatment with PTH stimulates bone formation. However, molecular mechanisms PTH-mediated osteoblast differentiation cell proliferation still not completely understood. In this study, we showed that induced endoplasmic reticulum (ER) stress in osteoblasts through PKR-like kinase (PERK)-eukaryotic initiation factor 2α...
Claudin 6 (CLDN6), a member of tight junction protein claudin (CLDN) family, inhibits proliferation and induces apoptosis MCF-7 breast cancer cells. However, these molecular mechanisms CLDN6-induced remain largely elusive. We previously found that restoration human CLDN6 gene expression was correlated with the level signal-regulating kinase 1 (ASK1) using cDNA array bioinformatics analysis. ASK1, mitogen-activated kinase, is involved in environmental stress-activation c-jun N-terminal (JNK)...
Although DNA damage repair plays a critical role in cancer chemotherapy, the function of lncRNAs this process remains largely unclear. In study, silico screening identified H19 as an lncRNA that potentially response and sensitivity to PARP inhibitors. Increased expression is correlated with disease progression poor prognosis breast cancer. cells, forced promotes resistance inhibition, whereas depletion diminishes increases exerted its functional roles via direct interaction ILF2 cell...
Reversible Nε-lysine acetylation has emerging as an important metabolic regulatory mechanism in microorganisms. Herein, we systematically investigated the site-specific and kinetic characterization of enzymatic (lysine acetyltransferase) nonenzymatic (AcP-dependent or Acyl-CoA-dependent), well their different effect on activity enzyme (AMP-forming acetyl-CoA synthetase, Acs). It was found that Bacillus subtilis synthetase (BsAcsA) can be acetylated vitro either catalytically by lysine...
Sustainable renewable energy is being hotly debated globally because the continued use of finite fossil fuels now widely recognized as unsustainable. Microalgae potentially offer great opportunities for resolving this challenge. Abscisic acid (ABA), jasmonic (JA) and salicylic (SA) are involved in regulating many physiological properties have been used higher plants. To test if phytohormones an impact on accumulating lipid microalgae, ABA, JA SA were to induce two Chlorella strains present...
The antisense RNA (asRNA) strategy is commonly used to block protein expression and downregulate the contents of metabolites in several microorganisms. Here, we show that asRNA can also be gfp Bacillus subtilis TS1726, which could further utilized identification new genes functions. Via application this strategy, biotin carboxylase II encoded by yngH (GeneID 939474) was identified play a more significant role maintaining acetyl-CoA (ACCase) activity enhancing surfactin synthesis compared...
Summary Saccharopolyspora erythraea has three AMP‐forming acetyl‐CoA synthetases (Acs) encoded by acsA1 , acsA2 and acsA3 . In this work, we found that nitrogen response regulator GlnR can directly interact with the promoter regions of all genes activate their transcription in to availability. The typical GlnR‐binding boxes were identified regions. Moreover, activities Acs enzymes modulated reversible lysine acetylation (RLA) acetyltransferase AcuA NAD + ‐dependent deacetylase SrtN....