A5047351745- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
- Prenatal Screening and Diagnostics
- Genomic variations and chromosomal abnormalities
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- Alzheimer's disease research and treatments
- DNA Repair Mechanisms
- Pluripotent Stem Cells Research
- Cancer-related gene regulation
- FOXO transcription factor regulation
- Birth, Development, and Health
- Liver Disease Diagnosis and Treatment
- Selenium in Biological Systems
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Hippo pathway signaling and YAP/TAZ
- interferon and immune responses
- PI3K/AKT/mTOR signaling in cancer
- Genetic Syndromes and Imprinting
- Cell death mechanisms and regulation
- Wnt/β-catenin signaling in development and cancer
- Tumors and Oncological Cases
Jinan University
2019-2025
First Affiliated Hospital of Jinan University
2016-2024
Tianjin Centers for Disease Control and Prevention
2023
Zhuhai People's Hospital
2020-2022
Sichuan University
2022
Ruijin Hospital
2019
Shanghai Jiao Tong University
2019
Yunnan Maternal and Child Health
2018
Yale University
2014-2017
University of Colorado Boulder
2009-2016
Mitochondria are inherited maternally in most animals, but the mechanisms of selective paternal mitochondrial elimination (PME) unknown. While examining fertilization Caenorhabditis elegans, we observed that mitochondria rapidly lose their inner membrane integrity. CPS-6, a endonuclease G, serves as factor is critical for PME. We found CPS-6 relocates from intermembrane space to matrix after degrade DNA. It acts with maternal autophagy and proteasome machineries promote Loss cps-6 delays...
Abstract Endonuclease G (ENDOG), a mitochondrial nuclease, is known to participate in many cellular processes, including apoptosis and paternal elimination, while its role autophagy remains unclear. Here, we report that ENDOG released from mitochondria promotes during starvation, which find be evolutionally conserved across species by performing experiments human cell lines, mice, Drosophila C. elegans . Under Glycogen synthase kinase 3 beta-mediated phosphorylation of at Thr-128 Ser-288...
Abstract As a major risk factor to human health, obesity presents massive burden people and society. Interestingly, the obese status of parents can cause progeny’s lipid accumulation through epigenetic inheritance in multiple species. To date, many questions remain as how leads signals that are transmitted across generations. In this study, we establish nematode model C. elegans raised on high-fat diet (HFD) measurable accumulation, which transmit signal their multigenerational progeny....
Abstract Mitochondrial dysfunction is becoming one of the main pathology factors involved in etiology neurological disorders. Recently, mutations coiled-coil-helix-coiled-coil-helix domain containing 2 (CHCHD2) and 10 (CHCHD10) which encode two homologous proteins that belong to mitochondrial CHCH protein family, are linked Parkinson’s disease amyotrophic lateral sclerosis (ALS)/frontotemporal dementia (FTD), respectively. However, physiological pathological roles these twin have not been...
Abstract Infertility is a worldwide reproductive health problem and there are still many unknown etiologies of infertility. In recent years, increasing evidence emerged confirmed that epigenetic regulation played leading role in reproduction. However, the function m 6 A modification infertility remains unknown. Here we report METTL3-dependent methylation plays an essential female fertility via balancing estrogen progesterone signaling. Analysis GEO datasets reveal significant downregulation...
Emerging evidence has unveiled heterogeneity in phenotypic and transcriptional alterations at the single-cell level during oxidative stress senescence. Despite pivotal roles of cellular metabolism, a comprehensive elucidation metabolomic cells its connection with senescent status remains elusive. By integrating live imaging mass spectrometry (SCLIMS), we establish cross-modality technique capturing both metabolome individual cells. The SCLIMS demonstrates substantial among diverse levels....
Obesity is a metabolic syndrome worldwide that causes many chronic diseases. Recently, we found an antiobesity effect of flaxseed polysaccharide (FP), but the mechanism remains to be elucidated. In this study, rats were first induced develop obesity by being fed high-fat diet. The obese then control diet, AIN-93M (group HFD), or 10% FP diet FPD). body weight, fat, adipose tissue and liver sections, serous total triglycerides, levels fasting blood glucose in serum, insulin, inflammatory...
Heat stress–induced survival advantages can be transgenerationally transmitted through the epigenetic marks of N 6 -mA and H3K9me3.
Lycium barbarum glycopeptide (LbGp) is the main bioactive compound extracted from traditional Chinese medicine. L. berries and has been proven to have numerous health benefits, including antioxidative, anti-inflammatory, anticancer, cytoprotective activities. However, antiaging effect of LbGp remains unknown.The lifespan body movement C. elegans were used evaluate on span. The thrashing assay was determine role in Parkinson's disease. To investigate mechanisms LbGp-induced effects, we...
Abstract Endonuclease G (ENDOG), a nuclear-encoded mitochondrial intermembrane space protein, is well known to be translocated into the nucleus during apoptosis. Recent studies have shown that ENDOG might enter matrix regulate genome cleavage and replication. However, little about role of in cytosol. Our previous work showed cytoplasmic competitively binds with 14-3-3γ, which released TSC2 repress mTORC1 signaling induce autophagy. Here, we demonstrate could also release Rictor from 14-3-3γ...
Abstract CRISPR-mediated gene activation (CRISPRa) is a promising therapeutic editing strategy without inducing DNA double-strand breaks (DSBs). However, in vivo implementation of these CRISPRa systems remains challenge. Here, we report compact and robust miniCas9 activator (termed miniCAFE) for endogenous target genes. The system relies on recruitment an engineered minimal nuclease-null Cas9 from Campylobacter jejuni potent transcriptional activators to locus by single guide RNA. It enables...
Abstract PIWI-interacting RNAs (piRNAs) are small noncoding that regulate gene expression, yet their molecular functions in neurobiology unclear. While investigating neurodegeneration mechanisms using human α-syn(A53T) Tg and Aβ ;α-syn(A53T) pan-neuronal overexpressing strains, we unexpectedly observed dysregulation of piRNAs. RNAi screening revealed knock down piRNA biogenesis genes improved thrashing behavior; further, a tofu-1 deletion ameliorated phenotypic deficits transgenic strains....
beta-Amyloid peptide (Abeta42) is the core protein of amyloid plaque in Alzheimer disease. The intracellular accumulation Abeta42 endosomal/lysosomal system has been under investigation for many years, but direct link between and dysfunction still largely unknown. Here, we found that both vitro vivo, a major portion was tightly inserted into small peripherally associated with lysosomal membrane, whereas its soluble minimal. We also molecules membrane tended to form multiple oligomeric...
HBx is a multifunctional hepatitis B virus (HBV) protein that crucial for HBV infection and pathogenesis contributing cause of hepatocyte carcinogenesis. However, the host targets mechanisms action are poorly characterized. We show here expression in Caenorhabditis elegans induces both necrotic apoptotic cell death, mimicking an early event liver by HBV. Genetic biochemical analyses indicate interacts directly with B-cell lymphoma 2 (Bcl-2) homolog CED-9 (cell death abnormal) through Bcl-2...
In this study, the toxicity of two one-dimensional (1D) nanoparticles, halloysite nanotubes (HNTs) and chitin whiskers (ChNCs), was investigated in detail. Both vitro vivo models were applied to evaluate by cell viability staining, apoptosis assay, reactive oxygen species generation. Particularly, HNTs ChNCs compared using an model Caenorhabditis elegans; their assessed models, mouse bone marrow mesenchymal stem cells (mBMSCs) rat osteosarcoma (UMR-106). vitro, both exhibited low at...
The pyrimidine metabolism pathway has important biological functions; it not only maintains appropriate pools but also produces bioactive intermediate metabolites. In a previous study, we identified that the is associated with aging regulation. However, molecular mechanism by which regulates remains unclear. Here, investigated longevity effect of intermediates on Caenorhabditis elegans (C. elegans). Our results demonstrated supplementation some could extend lifespan C. elegans. addition,...
Noninvasive prenatal testing of common aneuploidies has become routine over the past decade, but monogenic disorders remains a challenge in clinical implementation. Most recent studies have inherent limitations, such as complicated procedures, lack versatility, and need for prior knowledge parental genotypes or haplotypes. To overcome these robust versatile next-generation sequencing-based cell-free DNA (cfDNA) allelic molecule counting system termed cfDNA barcode-enabled single-molecule...
Uric acid is a common metabolite found in mammals' serum.Recently, several metabolites have been identified that modulate aging, and uric levels are positively correlated with lifespan.However, the molecular mechanisms underlying this largely undefined.Here we show acid, an end product of purine metabolism, enhances resistance oxidative stress extends life span Caenorhabditis elegans (C.elegans).We variety pathways leads to upregulation genes required for acid-mediated extension.We find...