A5019291510- Reproductive biology and impacts on aquatic species
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Aquaculture Nutrition and Growth
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Genetics and Reproduction
- Fish Ecology and Management Studies
- Fish Biology and Ecology Studies
- Physiological and biochemical adaptations
- Aquaculture disease management and microbiota
- Zebrafish Biomedical Research Applications
- Reproductive Health and Technologies
- CRISPR and Genetic Engineering
- Reproductive System and Pregnancy
- Fish biology, ecology, and behavior
- nanoparticles nucleation surface interactions
- Renal and related cancers
- Epigenetics and DNA Methylation
- Aquatic life and conservation
- MicroRNA in disease regulation
- Pluripotent Stem Cells Research
- Assisted Reproductive Technology and Twin Pregnancy
- Water Quality Monitoring Technologies
- Biocrusts and Microbial Ecology
- Human-Animal Interaction Studies
Universidad de León
2014-2024
Vall d'Hebron Institut de Recerca
2022
Universidad de La Sabana
2019-2022
Instituto Español de Oceanografía
2003-2021
Institut Français de la Mer
2016
Universidad de León
2013-2015
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2013
Center of Regenerative Medicine in Barcelona
2008-2011
University of Algarve
2005-2007
Cryopreservation is of interest not only for fish farming but also the conservation and genetic improvement resources. This technique has been well established in some freshwater species mainly, salmonid, sturgeons carps, however, last decade research was focused marine species. The benefits sperm cryopreservation include: (i) synchronization gamete availability both sexes, (ii) economy; (iii) simplification broodstock management, (iv) transport gametes from different farms, (v) germplasm...
The objective of this study is to analyse the effect ingestion two selected antioxidant probiotics strains ( Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347) on sperm quality parameters in asthenozoospermic males after three six weeks administration. Nine men without any medical treatment under similar diet conditions participated study. individual samples was evaluated before (previous ingestion), during (after 3 6 ingestion) probiotic administration (3 finishing...
Abstract Background Dedifferentiation occurs naturally in mature cell types during epimorphic regeneration fish and some amphibians. also the induction of pluripotent stem cells when a set transcription factors ( Oct4, Sox2, Klf4 c-Myc ) is over expressed types. Results We hypothesised that there are parallels between dedifferentiation or reprogramming somatic to induced natural process regeneration. analysed expression levels most commonly used pluripotency associated regenerating...
Cryopreservation is an important tool routinely employed in Assisted Reproduction Technologies (ARTs) and germplasm banking. For several years, the assessment of global DNA fragmentation seemed to be enough ensure integrity genetic material. However, cryopreservation can produce molecular alterations key genes transcripts undetectable by traditional assays, such modifications could interfere with normal embryo development. We used zebrafish as a model study effect on genes. optimized...
Sperm cryopreservation is widely used in clinic for insemination, vitro fertilization and other procedures such as intracytoplasmic sperm injection. The assessment after freezing/thawing of spermatozoa viability, motility sometimes DNA integrity (mainly using fragmentation assays) has been considered enough to guarantee the safety effectiveness technique. However, it known that, even when absent, a significant damage could be detected some genome regions. This particularly important...
Abstract The effects of stress during early vertebrate development can be especially harmful. Avoiding stressors in fish larvae is essential to ensure the health adult and their reproductive performance overall production. We examined consequences direct exposure successive acute development, including on miR-29a its targets, survival, hatching malformation rates, larval behaviour cartilage eye development. Our aim was shed light pleiotropic early-induced this model species. results showed...
Infertility is a medical concern worldwide and could also have economic consequences in farmed animals. Developing an efficient diet supplement with immediate effects on sperm quality promising tool for human reproduction domesticated animal species. This study aims at elucidating the effect of short-time probiotic supplementation consisting mixture two bacteria proven antioxidant anti-inflammatory activities zebrafish male behavior. For this purpose, three homogeneous groups males terms...
There is increasing evidence that gut microbiome could have effects on neurological processes and behavior. In this study we used the novel tank test (NTT) to analyze zebrafish exploring behavior after four months' supplementation with probiotics probed antioxidant anti-inflammatory properties. Results showed prolonged ingestion of Lactobacillus rhamnosus CECT8361 Bifidobacterium longum CECT7347 significantly alters swimming pattern mean speed in model. After treatment, strongly reduced...
Non-invasive manipulation of multicellular systems is important for medical and biological research. The ability to introduce, remove, or modify molecules in the intracellular environment pivotal our understanding cellular structure function. Herein, we report on an alternative method introducing foreign material into developing embryos using application femtosecond (fs) laser pulses. When intense fs pulses are focused a sub-micron spot, transient pores formed, providing transport pathway...
Targeted genomic manipulation using embryonic stem (ES) cells has not yet been achieved in zebrafish, although methods for zebrafish ES cell culture described literature. The knowledge of pluripotency markers this species is almost nonexistent and a very limiting factor the definition ideal conditions cells. Here, we studied expression several genes associated with versus differentiated some these recorded throughout development. Some commonly accepted are also tested cells, transient...