Abstract This paper presents an efficient approach for a computer-based solution to the problem of selection 'optimum robot' specifically aid industries. The breaks new ground by applying Multiple Attribute Decision Making (MADM) ideology equipment (robots) which is consequence because implicit aspect design. Indeed, also applicable robot design problem. procedure allows rapid convergence from very large number manageable shortlist potentially suitable robots using 'elimination search'...

Previous studies have suggested that embryonic vascular endothelial, endocardial and myocardial lineages originate from multipotential cardiovascular progenitors. However, their existence in vivo has been debated molecular mechanisms regulate specification of different are poorly understood. An ETS domain transcription factor Etv2/Etsrp/ER71 recently established as a crucial regulator endothelial differentiation zebrafish mouse embryos. In this study, we show etsrp-expressing...

Membrane surgery and nanosurgical cell isolation using high-intensity femtosecond laser pulses is reported. We demonstrate the applicability of ultrashort (femtosecond) for performing on live mammalian cells. When sub-10 were focused onto cell, precise sub-micron surgical cuts made biological membrane. Traversing cells relative to spot, we report localized ablation focal adhesion adjoining epithelial In each study, was conducted without morphologically compromising © 2005 Wiley-Liss, Inc.

Non-invasive manipulation of multicellular systems is important for medical and biological research. The ability to introduce, remove, or modify molecules in the intracellular environment pivotal our understanding cellular structure function. Herein, we report on an alternative method introducing foreign material into developing embryos using application femtosecond (fs) laser pulses. When intense fs pulses are focused a sub-micron spot, transient pores formed, providing transport pathway...

Femtosecond (fs) laser pulses have recently received wide interest as an alternative tool for manipulating living biological systems. In various model organisms the excision of cellular components and intracellular delivery foreign exogenous materials been reported. However, effect applied fs on cell viability development has yet to be determined. Using zebrafish (Danio rerio) our animal system, we address both short- long-term developmental changes following surgery embryonic cells.An...

Abstract Non‐invasive manipulation of live cells is important for cell‐based therapeutics. Herein we report on the uniqueness using high‐intensity femtosecond laser pulses reversibly permeabilizing mammalian biopreservation applications. When were suspended in a impermeable hyperosmotic cryoprotectant sucrose solution, used to transiently permeabilize cytoplasmic solute uptake. The kinetics exposed 0.2, 0.3, 0.4, and 0.5 M sucrose, following permeabilization, measured video microscopy,...

Mutations in cardiac actin (ACTC) have been associated with different abnormalities humans, including dilated cardiomyopathy and septal defects. However, it is still poorly understood how altered ACTC structure affects cardiovascular physiology results the development of distinct congenital disorders. A zebrafish mutant (s434 mutation) was identified that displays blood regurgitation a heart lacks endocardial cushion (EC) formation. We mutation as single nucleotide change alpha-cardiac 1a...

Abstract Pre-labour uterine contractions, occurring throughout pregnancy, are an important phenomenon involving the placenta in addition to myometrium. They alter environment and thus potentially blood supply fetus may provide crucial insights into processes of labour. Assessment in-vivo is however restricted due their unpredictability inaccessible nature utero-placental compartment. While clinical cardiotocography (CTG) only allows global, pressure-based assessment, functional magnetic...

Background : The lateral ganglionic eminence (LGE) in the ventral telencephalon is a diverse progenitor domain subdivided by distinct gene expression into dorsal region (dLGE) that gives rise to olfactory bulb and amygdalar interneurons (vLGE) striatal projection neurons. homeobox gene, Gsx2 , an enriched marker of LGE expressed high low gradient ventricular zone (VZ) as development proceeds. Aside from Gsx2, markers restricted VZ dLGE and/or vLGE remain largely unknown. Results Here, we...

Progenitors in the dorsal lateral ganglionic eminence (dLGE) are known to give rise olfactory bulb (OB) interneurons and intercalated cells (ITCs) of amygdala. The dLGE enriched transcription factor Sp8 is required for normal generation ITCs as well OB interneurons, particularly calretinin (CR)-expressing subtype. In this study, we used a genetic gain-of-function approach mice examine roles plays controlling development dLGE-derived neuronal subtypes. Misexpression throughout ventral...

The ability to differentially label single cells has important implications in developmental biology. For instance, determining how hematopoietic, lymphatic, and blood vessel lineages arise developing embryos requires fate mapping lineage tracing of undifferentiated precursor cells. Recently, photoactivatable proteins which include: Eos1, 2, PAmCherry3, Kaede4-7, pKindling8, KikGR9, 10 have received wide interest as cell probes. fluorescence spectrum these photosensitive can be easily...

Reducing X-ray dose increases safety in cardiac electrophysiology procedures but also image noise and artifacts which may affect the discernibility of devices anatomical cues. Previous denoising methods based on convolutional neural networks (CNNs) have shown improvements quality low-dose fluoroscopy images compromise clinically important details required by cardiologists.

The ability to differentially label single cells has important implications in developmental biology. For instance, determining how hematopoietic, lymphatic, and blood vessel lineages arise developing embryos requires fate mapping lineage tracing of undifferentiated precursor cells. Recently, photoactivatable proteins which include: Eos1, 2, PAmCherry3, Kaede4-7, pKindling8, KikGR9, 10 have received wide interest as cell probes. fluorescence spectrum these photosensitive can be easily...

Herein, we demonstrate the application of high-intensity femtosecond (fs) laser pulses for performing surgery on embryonic cells developing zebrafish (<i>Danio rerio</i>). When fs were focused onto individual blastomeres, transient pores formed exposing extracellular space to intracellular environment. Utilizing as a pathway delivery exogenous material, both chorionated and dechorionated embryos successfully loaded with fluorescent reporter molecule (fluorescein isothiocyanate (FITC))....

Non-invasive manipulation of live cells is important for cell-based therapeutics. Herein, we report on the application femtosecond laser pulses cellular manipulation, and generation optical pores cytoplasmic delivery non-reducing cryoprotectants. Under precise focusing, demonstrate membrane surgery mammalian cells, ablation focal adhesions adjoining fibroblast cells. In both studies, morphology cell post-laser treatment was maintained with no visible collapse or disassociation. When were...

We report the application of high-intensity femtosecond laser pulses as a novel tool for manipulating biological specimens. When were focused to near diffraction-limited focal spot, cellular material within volume was surgically ablated. Several dissection cuts made in membrane live mammalian cells, and surgery accomplished without inducing cell collapse or disassociation. By altering how applied, adhesions joining epithelial cells removed, resulting single isolation. To further examine...