A5021811766- Demographic Trends and Gender Preferences
- Birth, Development, and Health
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Animal Genetics and Reproduction
- Nutrition, Genetics, and Disease
- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Assisted Reproductive Technology and Twin Pregnancy
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Congenital heart defects research
- Renal and related cancers
- Biomedical Ethics and Regulation
- Tissue Engineering and Regenerative Medicine
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Sperm and Testicular Function
- Sexual Differentiation and Disorders
- Reproductive System and Pregnancy
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
University of Nottingham
2021-2024
Centro Regional de Selección y Reproducción Animal
2019-2021
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2018-2021
University College Dublin
2019
ABSTRACT Despite four decades of effort, robust propagation pluripotent stem cells from livestock animals remains challenging. The requirements for self-renewal are unclear and the relationship cultured to resident in embryo uncertain. Here, we avoided using feeder or serum factors provide a defined culture microenvironment. We show that combination activin A, fibroblast growth factor Wnt inhibitor XAV939 (AFX) supports establishment continuous expansion cell lines porcine, ovine bovine...
Abstract Cell-fate decisions during mammalian gastrulation are poorly understood outside of rodent embryos. The embryonic disc pig embryos mirrors humans, making them a useful proxy for studying gastrulation. Here we present single-cell transcriptomic atlas gastrulation, revealing cell-fate emergence dynamics, as well conserved and divergent gene programs governing early porcine, primate, murine development. We highlight heterochronicity in extraembryonic cell-types, despite the broad...
Highlights•ZRSR1mu produces severe defects in erythrocytes, muscle stretch, and spermatogenesis•Spermatogenesis of ZRSR1mu are due to splicing alteration U12-type intron•ZRSR1mu causes retention most the introns examined•ZRSR1 is implicated also removal U2 positioned adjacent a U12 intronSummaryThe U2AF35-like ZRSR1 has been recognition 3′ splice site during spliceosome assembly, but knockout mice do not show abnormal phenotypes. To analyze function its precise role RNA splicing, we...
Alternative splicing (AS) may play an important role in gonadal sex determination (GSD) mammals. The present study was designed to identify differentially expressed isoforms and AS modifications accompanying GSD mice.Using deep RNA-sequencing, we performed a transcriptional analysis of XX XY gonads during on embryonic days 11 (E11) 12 (E12). Analysis genes (DEG) identified hundreds related early differentiation that represent good candidates for reversal. Expression at time point E11 males...
ZRSR2 is a splicing factor involved in recognition of 3'-intron splice sites that frequently mutated myeloid malignancies and several tumors; however, the role mutations
Abstract Background Prior work in mice has shown that some retrotransposed elements remain substantially methylated during DNA methylation reprogramming of germ cells. In the pig, however, information about this process is scarce. The present study was designed to examine profiles porcine cells time course epigenetic reprogramming. Results Sows were artificially inseminated, and their fetuses collected 28, 32, 36, 39, 42 days later. At each point, genital ridges dissected from mesonephros...
Abstract Early mammalian gastrulation’s cell-fate decisions are poorly understood due to difficulties obtaining non-rodent embryos. The bilaminar disc of pig embryos mirrors humans, making them a useful proxy for studying gastrulation. Here we present single-cell transcriptomic atlas gastrulation, revealing emergence dynamics, as well conserved and divergent gene programs governing early porcine, primate, murine development. We highlight heterochronicity in extraembryonic cell-type...
Abstract Most current knowledge of sex determination in mammals has emerged from mouse and human studies. To investigate the molecular regulation process cattle, we used an RNA sequencing strategy to analyze transcriptome landscape male female bovine fetal gonads collected vivo at key developmental stages: before, during, after SRY gene activation on days D35 (bipotential gonad formation), D39 (peak expression), D43 (early differentiation). Differentially expressed genes (DEGs) were...
Abstract We hypothesized that sexually dimorphic differences exist in the expression of miRNAs amniotic fluid (AF) and maternal blood plasma (MP) association with process sex determination gonad differentiation cattle. Amniotic MP were collected from six pregnant heifers (three carrying a single male three female embryo) following slaughter on Day 39 postinsemination, coinciding peak SRY expression. Samples (six AF MP) profiled using an miRNA Serum/Plasma Focus PCR Panel. Differentially...
In most eutherian mammals, sex determination is the process through which a bipotential gonad (also known as genital ridges) develops into testis or ovary depending on chromosome content of embryo, specifically by presence SRY/Sry gene (sex-determining region Y chromosome). MicroRNAs (miRNAs) are short noncoding RNAs that regulate expression and involved in diverse functional roles including development, differentiation, apoptosis, immunity. We hypothesised miRNAs amniotic fluid (AF)...
Zrsr2 has been attributed a role in splice site recognition of U12 and U2 introns. To better understand the part played by minor splicing during oogenesis early embryo development, we produced mutant mice (Zrsr2mu). In Zrsr2mu female was blocked at secondary follicle stage, similar way as Zrsr1mu affects spermatogenesis. follicles showed altered expression genes related to gonad DNA replication cell cycle regulation, events involving enrichment U12-type intron retention (IR). Embryo...