A5100660130- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- Reproductive System and Pregnancy
- Genetic and phenotypic traits in livestock
- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Prenatal Screening and Diagnostics
- Sperm and Testicular Function
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Multiple Myeloma Research and Treatments
- Effects of Environmental Stressors on Livestock
- Neuroblastoma Research and Treatments
- Extracellular vesicles in disease
- Animal Genetics and Reproduction
- MicroRNA in disease regulation
- Hematological disorders and diagnostics
- Ruminant Nutrition and Digestive Physiology
- Genomic variations and chromosomal abnormalities
- Adipose Tissue and Metabolism
- Genetic Syndromes and Imprinting
- Congenital Anomalies and Fetal Surgery
- Medicine and Dermatology Studies History
Andalusian Institute of Agricultural and Fisheries Research and Training
2023-2025
University College Dublin
2016-2025
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2021-2025
Institute of Agrifood Research and Technology
2021-2024
Consejo Superior de Investigaciones Científicas
2022-2024
Hospital Universitario de Canarias
2002-2024
Hospital Clínico San Carlos
2024
Hospital Universitario 12 De Octubre
2024
Hospital San Pedro de Alcántara
2024
Biomar Microbial Technologies (Spain)
2023
To improve endometrial quality and implantation rates after the administration of platelet-rich plasma in patients with refractory endometrium.19 undergoing vitro fertilization, aged between 33 45 years a history endometrium, to whom platelet rich was given by infusion catheter into uterine cavity on tenth day hormone replacement therapy, then 72 hours first administration.Endometrial thicknesses >7mm reported use; all cases, >9mm were evident second administration. The entire study group...
Over 60% of relapsed/refractory (R/R) large B-cell lymphoma (LBCL) patients who receive chimeric antigen receptor (CAR) T cells will experience disease progression. There is no standard next line therapy and information in this setting scarce heterogeneous. We analyzed 387 R/R LBCL progressed after CAR from July 2018 until March 2022 Spain the United Kingdom. Median overall survival (OS) was 5.3 months, with significant differences according to interval between infusion progression (<2...
This study investigated bovine conceptus-induced modifications to the endometrial transcriptome related effects of interferon tau (IFNT), conceptus origin (in vivo vs. in vitro), and sex. In vitro (IVF) or (superovulation artificial insemination, AI) produced blastocysts were transferred into recipient heifers on day 7 estrous cycle. On 15, IVF- AI-derived conceptuses obtained by uterine flushing individually placed explants media for 6 h. Explants also cultured with alone as a control...
The objectives of this study were (i) to determine whether blastocyst-induced responses in endometrial explants detectable after 6- or 24-h co-culture vitro ; (ii) test if direct contact is required between embryos and the surface order stimulate gene expression; (iii) establish number blastocysts elicit a response; (iv) investigate upregulation five interferon-stimulated genes (ISGs) endometrium was specific blastocyst stage (v) alterations expression can be induced by...
Abstract This study combined in vitro production of bovine blastocysts, multiple embryo transfer techniques, and a conceptus-endometrial explant co-culture system to test the hypothesis that endometrium exposed long vs. short day 15 conceptuses would exhibit different transcriptome profile reflective potential for successful pregnancy establishment. Bovine endometrial explants collected at late luteal stage estrous cycle were cultured RPMI medium 6 h with nothing (control), 100 ng/mL...
Abstract Background Bovine respiratory disease (BRD) is the main cause of mortality among 1-to-5 month old calves in Ireland, accounting for approximately one-third deaths. Despite widespread use clinical signs diagnosing BRD, lung lesions are detected, using thoracic ultrasonography (TUS) or following post-mortem, showing no signs. This highlights limitation as a method detecting sub-clinical BRD. Using 53 purchased artificially-reared male dairy calves, objectives this study were to: (i)...
<h3>OBJECTIVES</h3> Several lines of evidence suggest that the endothelial constitutive nitric oxide synthase (ecNOS) and angiotensin converting enzyme (ACE) may have a role in Alzheimer9s disease. ACE is widely expressed brain, DNA polymorphism at gene has been linked to risk for late onset Nitric (NO) production by microglial cells, astrocytes, brain microvessels enhanced patients with There growing NO involved neuronal death disease, oxidative stress caused could be pathogenic mechanism...
Pregnancy establishment in cattle is contingent on conceptus elongation—a fundamental developmental event coinciding with the time during which most pregnancies fail. Elongation vivo directly driven by uterine secretions, indirectly influenced systemic progesterone concentrations, and has yet to be recapitulated vitro. To better understand microenvironment evolved facilitate this phenomenon, amino acid carbohydrate composition of fluid was interrogated using high-throughput metabolomics days...
Successful bovine pregnancy establishment hinges on conceptus elongation, a key reproductive phenomenon coinciding with the period during which most pregnancies fail. Elongation is yet to be recapitulated in vitro, whereas vivo it directly driven by uterine secretions and indirectly influenced prior circulating progesterone levels. To better understand microenvironment evolved facilitate this fundamental developmental event, fluid was recovered Days 12-14 of oestrous cycle - window...
<h2>ABSTRACT</h2> The objective was to compare pregnancy per service event (P/S) in lactating dairy cows following timed artificial insemination (<b>AI</b>) or embryo transfer (<b>ET</b>) using either fresh frozen vitro produced (<b>IVP</b>) embryos. Oocytes were collected once week for up 9 weeks transvaginal ovum pick-up from elite donors (ET-DAIRY; n=40; Holstein-Friesian and Jersey) beef (ET-ELITE-BEEF; n=21; Angus). Both ET-DAIRY ET-ELITE-BEEF comprised of heifers cows. In addition,...
The study of early maternal-embryonic cross-talk remains one the most challenging topics in reproductive biology. Understanding physiological mechanisms involved interactions between maternal tract and developing embryo is essential for enhancing bovine efficiency. This complex communication starts within oviduct, where modulation biological processes important ensuring quality partially facilitated through extracellular vesicles (EVs). Utilizing a combination vivo vitro models this had...
The objective of this study was to determine the effect timing presumptive conceptus attachment (pCA) and its relationship with subsequent pregnancy loss in lactating dairy cows following timed artificial insemination conventional (TAI-C) or X-sorted (TAI-S) semen embryo transfer (TET) frozen-thawed vitro-produced (IVP) embryos. Lactating (n = 417, predominantly Holstein-Friesian) located 3 herds were blocked based on parity, calving date economic breeding index randomly assigned receive...
Suboptimal concentrations of circulating progesterone (P4) in the early postovulatory period have been associated with low fertility observed high-producing lactating dairy cows. The administration human chorionic gonadotropin (hCG) increases P4 cattle by stimulating its endogenous production corpus luteum (CL) and creating an accessory CL if administered at appropriate stage cycle. aim this study was to evaluate effect a single hCG on d 2 estrus cycle pregnancy per artificial insemination...
The role played by a sustained angiogenesis in cancer and other diseases stimulates the interest search for new antiangiogenic drugs. In this manuscript, we provide evidence that 1,8- dihydroxy-9,10-anthraquinone (danthron), isolated from fermentation broth of marine fungus Chromolaenicola sp. (HL-114-33-R04), is inhibitor angiogenesis. results obtained with vivo CAM assay indicate danthron potent compound. vitro studies human umbilical endothelial cells (HUVEC) reveal anthraquinone inhibits...
Abstract Conceptus elongation coincides with one of the periods greatest pregnancy loss in cattle and is characterized by rapid trophectoderm expansion, commencing ~ Day 13 pregnancy, i.e . before maternal recognition. The process has yet to be recapitulated vitro does not occur absence uterine gland secretions vivo Moreover, conceptus rates are positively correlated systemic progesterone circulation. It is, therefore, a maternally-driven progesterone-correlated developmental phenomenon....
Abstract Conceptus elongation is a fundamental developmental event coinciding with period of significant pregnancy loss in cattle. The process has yet to be recapitulated vitro, whereas vivo it directly driven by uterine secretions and indirectly influenced systemic progesterone. To better understand the environment facilitating this critical reproductive phenomenon, we interrogated biochemical composition luminal fluid from heifers high vs physiological circulating progesterone on days...
A growing body of evidence suggests that paternal factors have an impact on offspring development. These studies been mainly carried out in mice, where seminal plasma (SP) has shown to regulate endometrial gene expression and embryo development subsequent health. In cattle, infusion SP into the uterus also induces changes expression, however, for effect early is lacking. addition, during natural mating, bull ejaculates vagina; hence, it not clear whether any reaches this species. Thus, aim...
We report our experience in a cytogenetic study of 93 spontaneous abortions. Specimens were obtained by transabdominal chorionic villus sampling (TACVS) women requesting prenatal diagnosis (CVS) but whom an arrested pregnancy had been diagnosed during the ultrasound examination. Our success rate, i.e. percentage cases where we results, was 91.4 per cent, and rate abnormalities—mostly aneuploidies polyploidies—was 62.3 cent. In normal cases, masculine:feminine ratio 1:1. These results confirm...