A5030683512- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Cardiovascular Disease and Adiposity
- Circular RNAs in diseases
- Liver Disease Diagnosis and Treatment
- Porphyrin Metabolism and Disorders
- Neonatal Health and Biochemistry
- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA Interference and Gene Delivery
- Liver Diseases and Immunity
- Fish Biology and Ecology Studies
- Plant nutrient uptake and metabolism
- Pregnancy and preeclampsia studies
- Lipid Membrane Structure and Behavior
- RNA modifications and cancer
- Metabolism and Genetic Disorders
- Urologic and reproductive health conditions
- Heme Oxygenase-1 and Carbon Monoxide
- Nanopore and Nanochannel Transport Studies
- Ichthyology and Marine Biology
- Reproductive System and Pregnancy
- Field-Flow Fractionation Techniques
- Frailty in Older Adults
- Plant Micronutrient Interactions and Effects
- Cardiac Arrhythmias and Treatments
CIC bioGUNE
2016-2025
Hospital San Agustin
2024-2025
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2014-2024
Universidad de Bogotá Jorge Tadeo Lozano
2008-2023
Hospital de Basurto
2021
Centro de Investigación Biomédica en Red
2017-2019
Centro Nacional de Biotecnología
2005-2011
Consejo Superior de Investigaciones Científicas
2004-2011
Weatherford College
2010
Harris Birthright Research Centre for Fetal Medicine
2010
Cholangiocarcinoma (CCA) includes a heterogeneous group of biliary cancers with poor prognosis. Several conditions, such as primary sclerosing cholangitis (PSC), are risk factors. Noninvasive differential diagnosis between intrahepatic CCA and hepatocellular carcinoma (HCC) is sometimes difficult. Accurate noninvasive biomarkers for PSC, CCA, HCC not available. In the search novel biomarkers, serum extracellular vesicles (EV) were isolated from (n = 43), PSC 30), or 29) patients healthy...
Abstract In Arabidopsis thaliana, the PHOSPHATE TRANSPORTER1 (PHT1) family encodes high-affinity phosphate transporters. They are transcriptionally induced by starvation and require TRANSPORTER TRAFFIC FACILITATOR (PHF1) to exit endoplasmic reticulum (ER), indicating intracellular traffic as an additional level of regulation PHT1 activity. Our study revealed that PHF1 acts on PHT1, upstream vesicle coat protein COPII formation, regulatory events occur during trafficking determine its ER...
BackgroundFibromyalgia is a complex, relatively unknown disease characterised by chronic, widespread musculoskeletal pain. The gut-brain axis connects the gut microbiome with brain through enteric nervous system (ENS); its disruption has been associated psychiatric and gastrointestinal disorders. To gain an insight into pathogenesis of fibromyalgia identify diagnostic biomarkers, we combined different omics techniques to analyse serum composition.MethodsWe collected faeces blood samples...
PHOSPHATE TRANSPORTER1 (PHT1) genes encode phosphate (Pi) transporters that play a fundamental role in Pi acquisition and remobilization plants. Mutation of the Arabidopsis thaliana TRANSPORTER TRAFFIC FACILITATOR1 (PHF1) impairs transport, resulting constitutive expression many starvation-induced genes, increased arsenate resistance, reduced accumulation. PHF1 was detected all tissues, particularly roots, flowers, senescing leaves, induced by starvation, thus mimicking patterns whole PHT1...
Extracellular vesicle (EV)-mediated glia-to-neuron communication has been recognized in a growing number of physiological and pathological situations. They transport complex sets molecules that can be beneficial or detrimental for the receiving cell. As other areas biology, their analysis is revolutionizing field neuroscience, since fundamental signalling processes are being re-evaluated, applications neurodegenerative disease therapies have emerged. Using human astrocytic differentiated...
Urine contains extracellular vesicles (EVs) that concentrate molecules and protect them from degradation. Thus, isolation characterisation of urinary EVs could increase the efficiency biomarker discovery. We have previously identified proteins RNAs with differential abundance in prostate cancer (PCa) patients compared to benign hyperplasia (BPH). Here, we focused on analysis metabolites contained collected PCa BPH. Targeted metabolomics was performed by ultra-high-performance liquid...
Extracellular vesicles (EVs) are important mediators of cell-cell communication in a broad variety physiological contexts. However, there is ambiguity around the fundamental mechanisms by which these effects transduced, particularly relation to their uptake recipient cells. Multiple modes cellular entry have been suggested and we further explored role glycans as potential determinants uptake, using EVs from murine hepatic cell lines AML12 MLP29 independent yet comparable models. Lectin...
There is a compelling clinical imperative to identify discerning molecular biomarkers of hepatic disease in order inform the diagnosis, prognosis and treatment.We have investigated proteome urinary vesicles present urine samples obtained from experimental models for study liver injury, as an approach identifying potential disease.The biochemical proteomic characterization highly purified exosome-like has identified 28 proteins previously unreported these vesicles, many that been associated...
: Cholangiocarcinoma (CCA) comprises a group of heterogeneous biliary cancers with dismal prognosis. The etiologies most CCAs are unknown, but primary sclerosing cholangitis (PSC) is risk factor. Non-invasive diagnosis CCA challenging and accurate biomarkers lacking. We aimed to characterize the transcriptomic profile serum urine extracellular vesicles (EVs) from patients CCA, PSC, ulcerative colitis (UC), healthy individuals. Serum EVs were isolated by serial ultracentrifugations...
Salivary extracellular vesicles (EVs) represent an attractive source of biomarkers due to the accessibility saliva and its non-invasive sampling methods. However, lack comparative studies assessing efficacy different EV isolation techniques hampers use salivary EVs in clinical settings. Moreover, effects age on are largely unknown, hindering identification EV-associated across lifespan. To address these questions, we compared concentration, size mode, protein purity using eight before after...
Abstract Hepatocytes release extracellular vesicles (EVs) loaded with signaling molecules and enzymes into the bloodstream. Although importance of EVs in intercellular communication is already recognized, metabolic impact carried by these still unclear. We evaluated global effect enzymatic activities performing untargeted metabolomic profiling serum samples after their exposure to EVs. This approach revealed a significant change abundance 94 signals. Our study shows that modify concentration...
// Felix Royo 1, 2, * , Patricia Zuñiga-Garcia Verónica Torrano Ana Loizaga 3, Pilar Sanchez-Mosquera 1 Aitziber Ugalde-Olano 4 Esperanza González R. Cortazar Laura Palomo Sonia Fernández-Ruiz Isabel Lacasa-Viscasillas 3 Maria Berdasco 5 James D. Sutherland Rosa Barrio Amaia Zabala-Letona Natalia Martín-Martín Arruabarrena-Aristorena Lorea Valcarcel-Jimenez Alfredo Caro-Maldonado Jorge Gonzalez-Tampan Guido Cachi-Fuentes Manel Esteller M. Aransay 2 Miguel Unda Juan Falcón-Pérez 6, Ω Arkaitz...
Abstract Extracellular vesicles (EVs) are secreted nanostructures that play various roles in critical cancer processes. They operate as an intercellular communication system, transferring complex sets of biomolecules from cell to cell. The concentration EVs is difficult decipher, and there unmet technological need for improved (faster, simpler, gentler) approaches isolate matrices. Herein, acoustofluidic extracellular (ACEV) presented, based on a thin‐film printed circuit board with...
The discovery that the cells communicate through emission of vesicles has opened new opportunities for better understanding physiological and pathological mechanisms. This also provides a novel source non-invasive disease biomarker research. Our group previously reported hepatocytes release extracellular with protein content reflecting cell-type origin. Here, we show released by carry RNA. We report messenger RNA composition in two non-tumoral hepatic models: primary culture rat progenitor...
Bladder cancer is one of the most common cancers and, together with prostate carcinoma, accounts for majority malignancies genitourinary tract. Since prognosis ameliorates early detection, it will be beneficial to have a repertoire diagnostic markers that could complement current diagnosis protocols. Recently, cell-secreted extracellular vesicles received great interest as source low invasive disease biomarkers because they are found in many body fluids, including urine. The work describes...