A5039314902- Reproductive Biology and Fertility
- Genetic and phenotypic traits in livestock
- Reproductive Physiology in Livestock
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Effects of Environmental Stressors on Livestock
- Reproductive System and Pregnancy
- Vascular Malformations and Hemangiomas
- Lymphatic System and Diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic Syndromes and Imprinting
- Renal and related cancers
- Lymphatic Disorders and Treatments
- Food Industry and Aquatic Biology
- Genetic Mapping and Diversity in Plants and Animals
- Ovarian function and disorders
- Folate and B Vitamins Research
- Prenatal Screening and Diagnostics
- Ruminant Nutrition and Digestive Physiology
- Agriculture Sustainability and Environmental Impact
- Epigenetics and DNA Methylation
- Congenital heart defects research
University of Nottingham
2021-2023
The University of Queensland
2005-2020
AgResearch
2010-2014
Developmental defects caused by targeted gene inactivation in mice are commonly subject to strain-specific modifiers that modulate the severity of phenotype. Although several genetic modifier loci have been mapped mice, gene(s) residing at these mostly unidentified, and molecular mechanisms action remain poorly understood. Mutations Sox18 cause a variable phenotype human congenital syndrome hypotrichosis-lymphedema-telangiectasia, Sox18-null varies from essentially normal completely devoid...
The objectives of this study were (i) to determine whether blastocyst-induced responses in endometrial explants detectable after 6- or 24-h co-culture vitro ; (ii) test if direct contact is required between embryos and the surface order stimulate gene expression; (iii) establish number blastocysts elicit a response; (iv) investigate upregulation five interferon-stimulated genes (ISGs) endometrium was specific blastocyst stage (v) alterations expression can be induced by...
Approximately one million in vitro produced (IVP) cattle embryos are transferred worldwide each year as a way to improve the rates of genetic gain. The most advanced programmes also apply genomic selection at embryonic stage by SNP genotyping and calculation estimated breeding values (GEBVs). However, high proportion fail establish pregnancy. Here, we demonstrate that further interrogation data collected for GEBVs can effectively remove aneuploid from pool, improving live births per embryo...
One-carbon (1C) metabolism provides methyl groups for the synthesis and/or methylation of purines and pyrimidines, biogenic amines, proteins, phospholipids. Our understanding how 1C pathways operate, however, pertains mostly to (rat) liver. Here we report that transcripts all bar two genes (i.e., BHMT, MAT1A) encoding enzymes in linked methionine-folate cycles are expressed cell types within ovarian follicle, oocyte, blastocyst cow, sheep, pig; as well rat granulosa cells (GCs) human KGN (a...
The aims of this study were (i) to investigate changes in the global transcriptome bovine endometrial explants induced by exposure blastocysts, (ii) if male and female blastocysts elicit a differential response vitro (iii) determine whether endometrium responds presence murine embryos. In Experiment 1, from same uterus cultured for 6 h with or without 20 vitro-produced blastocysts. 2, produced IVF either using sex-sorted semen conventional unsorted followed embryo sexing based on biopsy. 3,...
Experiments were designed to investigate the effect of different doses and timing an eCG treatment given during GnRH-based synchronization protocols on follicular dynamics fertility in cattle. In Exp. 1, Angus heifers (n = 50) received a 7-d Ovsynch + progesterone protocol (on d 0, GnRH insert administered; 7, was removed PGF2α injected; 9.5, injected 56 h after removal) with (0, 300, 500, 700, or 1,000 IU) administered 7. 2, cows 27) same as 1 assigned randomly receive 0 400 IU i.m. 2 3,...
Background In vitro maturation (IVM) of germinal vesicle intact oocytes prior to in fertilization (IVF) is practiced widely animals. human assisted reproduction it generally reserved for fertility preservation or where ovarian stimulation contraindicated. Standard practice incorporates complex proteins (CP), the form serum and/or albumin, into IVM media mimic follicle environment. However, undefined nature CP, together with batch variation and ethical concerns regarding their origin,...
Contents Two experiments were designed to investigate the administration of intravaginal progesterone in protocols for oestrus and ovulation synchronization beef heifers. In Experiment 1, cyclic Black Angus heifers (n = 20) received an Ovsynch protocol randomly assigned receive (CIDR‐Ovsynch) or not (Ovsynch) a device between Days 0 7. Treatment with controlled internal drug release (CIDR) significantly increased size dominant follicle prior (12.8 ± 0.4 CIDR‐Ovsynch vs 11.4 Ovsynch) (p <...
A study was designed to compare the effect of a prostaglandin-based synchronization protocol on ovarian follicular dynamics in sheep with FecB (Booroola) mutation. Forty dry Romney (57.6 ± 7.3 kg; 6.1 1.1 years) were randomly selected from both Invermay Booroola (BB; n = 20) and commercial (non-FecB carriers, ++; flocks. All ewes had their estrous cycles synchronized 2 i.m. injections PGF (150 μg cloprostenol, Estrumate, Schering-Plough Coopers Animal Health Ltd., New Zealand) administered 7...
Contents Increasing use of fixed‐time artificial insemination ( FTAI ) in beef cattle production has presented an opportunity for the fresh or chilled semen as alternative to standard cryopreserved semen. The objective this study was examine vitro sperm function and pregnancy rate electroejaculated semen, stored 48 hr, compared conventionally with optimized protocol Brahman cattle. Semen from three bulls collected, aliquots were extended either (at 5°C) frozen LN 2 a Tris‐egg yolk extender...
The cattle industry primarily employs embryo bisection in order to obtain genetic samples for pre-implantation screening and selection of embryos. Although practical rapid, is invasive adversely affects viability cryopreservation. An alternative biopsy approach aspirate the blastocoele fluid (referred as blastocentesis), which not only provides a sample, but also has potential improve cryopreservation (Palini et al. 2013 Repro. Biomed. 26, 603–610). This study investigates blastocentesis low...
An experiment was designed to evaluate the effect of different doses eCG on ovarian follicular dynamics in heifers treated with a Ovsynch plus progesterone protocol. Twenty-five cyclic yearling Black Angus (373.0 ± 35.7 kg), 2 replicates, received an injection 100 μg GnRH (Ovurelin, Bomac Laboratories Ltd., Auckland, New Zealand) i.m. and intravaginal device (1.38 g progesterone; Eazi-Breed CIDR, Pfizer Animal Health, Day 0 (beginning experiment), followed by 500 cloprostenol (PGF,...
During mammalian spermiogenesis nucleosomal histone proteins bound to DNA are replaced by protamines. The integrity and stability of the protamine-DNA association in sperm can be measured using chromatin structure assay (SCSA). recent emergence compelling information from assisted reproduction humans a relationship between establishment maintenance pregnancy has led considerable interest production recreational animals.1 aim present study was determine incidence instability amongst bulls...