A5063870112- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Reproductive Biology and Fertility
- Ovarian function and disorders
- Estrogen and related hormone effects
- Gynecological conditions and treatments
- Prenatal Screening and Diagnostics
- Pluripotent Stem Cells Research
- Uterine Myomas and Treatments
- Reproductive Physiology in Livestock
- Pregnancy and preeclampsia studies
- Reproductive Health and Technologies
- Endometrial and Cervical Cancer Treatments
- Genomics and Phylogenetic Studies
- Regulation of Appetite and Obesity
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Bacteriophages and microbial interactions
- Genomics and Rare Diseases
- Renal and related cancers
- Genomic variations and chromosomal abnormalities
- Pregnancy-related medical research
- Cancer Genomics and Diagnostics
- Preterm Birth and Chorioamnionitis
- Cancer Cells and Metastasis
Universidad Pablo de Olavide
2011-2022
Instituto Valenciano de la Edificación
2010-2021
Centro Oncológico de Galicia
2018
Complexo Hospitalario Universitario A Coruña
2018
Instituto de Investigación Biomédica de A Coruña
2018
CooperSurgical (United States)
2017
Hospital Universitario Miguel Servet
2011-2016
Universitat de València
2004-2015
Instituto Aragonés de Ciencias de la Salud
2015
Valencian Infertility Institute
2004-2014
Elevated serum progesterone levels at the end of follicular phase in controlled ovarian stimulation (COS) leads to a poorer ongoing pregnancy rate IVF cycles due reduced endometrial receptivity. The objective this study was use microarray technology compare gene expression profiles window implantation according circulating progesterone. For prospective cohort study, data were obtained from biopsies 12 young healthy oocyte donors undergoing COS with pituitary suppression by either...
Controlled ovarian hyperstimulation (COH) used in IVF produces lower implantation rates per embryo transferred compared to natural cycles utilized ovum donation, suggesting a suboptimal endometrial development. Endometrial receptivity has recently been investigated menstrual with the aid of microarray technology. The aim this study is investigate impact COH using urinary gonadotrophins long protocol GnRH agonists without progesterone supplementation (similar cycle) on gene expression...
SUMMARY Continuous research spanning more than three decades has made the Bacillus bacteriophage φ29 a paradigm for several molecular mechanisms of general biological processes, such as DNA replication, regulation transcription, phage morphogenesis, and packaging. The genome consists linear double-stranded (dsDNA), which terminal protein (TP) covalently linked to its 5′ ends. Initiation carried out by protein-primed mechanism, been studied in detail is considered be model system replication...
Controlled ovarian stimulation induces morphological, biochemical, and functional genomic modifications of the human endometrium during window implantation.Our objective was to compare gene expression profile in natural vs. controlled cycles throughout early-mid secretory transition using microarray technology.Microarray data from 49 endometrial biopsies obtained LH+1 LH+9 (n=25) chorionic gonadotropin (hCG) +1 hCG+9 (n=24) were analyzed different methods, such as clustering, profiling...
During reproductive life, the human endometrium undergoes around 480 cycles of growth, breakdown and regeneration should pregnancy not be achieved. This outstanding regenerative capacity is basis for women's cycling its dysfunction may involved in etiology pathological disorders. Therefore, endometrial tissue must rely on a remarkable somatic stem cells (SSC) population. Here we explore hypothesis that side population (SP) correspond to cells. We isolated, identified characterized SP...
Successful embryo implantation depends on the quality of embryo, as well receptivity endometrium. The aim this study was to investigate endometrial gene expression profile in women with unexplained infertility comparison fertile controls at time order find potential predictive markers uterine and identify molecular mechanisms infertility. High-density oligonucleotide arrays, comprising 44 000 targets, were used define infertile (n = 4) 5) during mid-secretory phase (day LH + 7). Microarray...
The endometrium, lining of the uterus, is a highly active organ that remodelled periodically during lifespan. Different studies suggest presence an adult or progenitor stem cell (PSC) population in this tissue because its cyclic regenerative capacity.In study, we aim at identifying and localizing putative PSC murine uterus using 5-bromo-2'-deoxyuridine (BrdU) labelling method to detect label-retaining cells (LRCs) cycle slowly. Uteri from BrdU-treated mice were analysed via single double...
There is an urgent need to develop optimized experimental models examine human implantation. These studies aimed (i) establish a endometrium-like three-dimensional (3D) culture system, and (ii) the attachment of trophoblast-like Jar spheroids culture. In present work, 3D endometrial cultures were constructed with fibrin-agarose as matrix scaffold, using epithelial stromal cells from both primary established cell lines. An assay between trophoblast was developed. Epithelial (cytokeratin+)...
Is it possible to determine the receptivity status of an endometrium by combined quantitative reverse transcription PCR (RT-qPCR) expression analysis genes involved in endometrial proliferation and immunity?The new ER Map®/ER Grade® test can predict RT-qPCR using a panel maternal immune response associated embryonic implantation.The human reaches receptive adequate for implantation around Days 19-21 menstrual cycle. During this period, known as window (WOI), shows specific gene profile...
This descriptive study evaluates the impact on endometrial development of standard and high doses a GnRH antagonist in stimulated cycles compared with agonist natural cycles.Thirty-one oocyte donors were treated combination rFSH 0.25 mg/day ganirelix (standard dose), 2 (high dose) or 0.6 buserelin (long protocol). Vaginal progesterone (200 mg/day) was administered luteal phase. Endometrial biopsies performed 7 days after HCG administration. Additional carried out subset 12 subjects,...
New approaches for non-invasive embryo-quality assessment are among the major goals in Reproductive Medicine. We hypothesize that detection of changes protein profile culture media which blastocysts cultured could be a potential indicator viability embryo and, thus, useful tool selecting more appropriate to transferred.Using protein-array technology, we analysed corresponding 24 h conditioned implanted versus those did not implant. A statistical approach was followed compare each these...
Identification of genes involved in trophoblast differentiation is great interest understanding cellular and molecular mechanisms placental development relevant clinically to fetal development, fertility, maternal health. Herein, we investigated human embryonic stem cells (hESCs) down the lineage by culture with bone morphogenetic protein 4 (BMP4) over a 10-day period. Within 2 days, stemness markers POU5F1 NANOG were markedly down-regulated, followed temporally up-regulation CDX2, KRT7,...
Eutopic endometrium in endometriosis has molecular evidence of resistance to progesterone (P4) and activation the PKA pathway stromal compartment. To investigate global temporal responses eutopic P4, we compared early (6-h), intermediate (48-h), late (14-Day) transcriptomes, signaling pathways, networks human endometrial fibroblasts (hESF) from women with (hESFendo) hESF without (hESFnonendo). Endometrial biopsy samples were obtained subjects mild peritoneal (n = 4 per group), isolated...
Intrinsic abnormalities in transplanted eutopic endometrium are believed to contribute the pathogenesis of pelvic endometriosis. Herein we investigated transcriptomic differences human endometrial stromal fibroblasts (hESFs) from women with (hESFendo) vs. without (hESFnonendo) endometriosis, response activation protein kinase A (PKA) pathway 8-bromoadenosine-cAMP (8-Br-cAMP). hESFnonendo (n = 4) and hESFendo were isolated treated ± 0.5 mm 8-Br-cAMP for 96 h. Purified total RNA was subjected...
The leptin system is implicated in the regulation of body weight and reproductive function, acting at endocrine paracrine levels. This ligand-receptor mandatory for embryonic implantation rodents. Here, we investigate expression pattern total receptor (OB-RT), long form (OB-RL) short isoforms HuB219.1 HuB219.3 human endometrium. Furthermore, studied OB-RT mRNA during preimplantation development endometrial OB-RL. Leptin its increase luteal phase peak late part. was localized epithelial...