A5082980693- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Animal Genetics and Reproduction
- Insect Utilization and Effects
- Genetic and phenotypic traits in livestock
- Aquaculture disease management and microbiota
- Animal and Plant Science Education
- Protein Hydrolysis and Bioactive Peptides
- Meat and Animal Product Quality
- Algal biology and biofuel production
- Neurobiology and Insect Physiology Research
- Animal Nutrition and Physiology
- Bee Products Chemical Analysis
- Effects of Environmental Stressors on Livestock
- Pneumocystis jirovecii pneumonia detection and treatment
- Agricultural and Food Production Studies
- HIV/AIDS drug development and treatment
- Aquatic life and conservation
- HIV Research and Treatment
- Seed Germination and Physiology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Rabbits: Nutrition, Reproduction, Health
- Animal Diversity and Health Studies
Technological Institute of Castilla y León
2016-2024
Hospital Reina Sofía de Murcia
2024
Instituto Tecnológico Agrario de Castilla y León
2020-2024
Agricultural Research Service
2019
Consejo Superior de Investigaciones Científicas
2019
Universitat de Miguel Hernández d'Elx
2019
Universitat Politècnica de Catalunya
2019
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2019
Instituto Valenciano de Investigaciones Agrarias
2008-2015
Junta de Castilla y León
2012
The demand of optimal protein for human consumption is growing. Food and Agriculture Organization (FAO) has highlighted aquaculture as one the most promising alternatives this supply gap due to high efficiency fish growth. However, been facing its own sustainability problem, because traditionally satisfied with use fishmeal (FM) main source. Some sustainable substitutes FM come from insects. present manuscript provides insight into an experiment carried out on rainbow trout (Oncorhynchus...
The aquaculture industry is diminishing the amount of fishmeal needed to maintain its protein demand. Alternatives are tested in this way, being insects one most promising. In study, two different insectmeals were at replacement proportions (150 and 300 g·kg-1) diets for Oncorhynchus mykiss. This study covers diverse aspects related growth, utilization, physiological status fish, quality final product. inclusion had no negative effects on utilization fish. At highest level with Tenebrio...
Hermetia illucens and Tenebrio molitor were tested on account of their potential to replace fish protein in feed. Two levels replacement for H. illucens, 30% 50% (H30 H50), one T. molitor, (T50), as well an additional diet with a modified fatty acid fraction (H50M), investigated relation juvenile Sparus aurata growth indices, enzyme activities gut microbiome. A T50 showed similar results control (C) diet, no significant differences regarding morphological indices minor nutritional indices....
Contents Cryopreserved boar sperm is not used extensively for artificial insemination, owing to the poor fertility rates of after freezing and thawing. The membrane damaged as cells are cooled from body temperature 5°C (cold shock), well during freeze–thaw process. Increasing cholesterol content membranes could help them survive cryopreservation, similar other species that cold shock sensitive. aim this study was determine optimal cholesterol‐loaded cyclodextrin (CLC) concentration use...
Rabbits constitute an interesting model to understand gamete interaction and test novel Artificial Reproductive Techniques, but in vitro fertilization (IVF) is particularly problematic this species. We have conducted a series of experiments develop consistent IVF technique. Initially, we checked viability, acrosome integrity, capacitation motility ejaculated sperm purified by density gradient incubated at different times three media: Tyrode's Albumin Lactate Pyruvate (TALP), human tubal...
The conservation of genetic resources in pig breeds, notably the Iberian pig, is crucial for improvement and sustainable production. Prolonged storage liquid nitrogen (LN
The aim of the present study was to determine whether levels reactive oxygen species (ROS) substances production and lipid peroxidation sperm membrane were related quality that ejaculates exhibited after cryopreservation in boars. Ejaculates from 42 healthy boars used this they cryopreserved with lactose-egg yolk extender (LEY). Several parameters assessed by flow cytometry samples incubated for 30 150 min at 37 °C thawing: percentage intact plasma (SIPM), intracellular through mean DCF...
The recovery of proteins from microalgae is gaining special attention for animal feed applications especially fish feed, as the costs aquaculture feeds represent between 40-70% produced. Besides, use pig manure to growth biomass could contribute bioremediation and therefore reduce environmental impacts its storage. objective this study was recover protein concentrates microalgal grown in manure, paying especial quality extracted that can be used source fish, amino acids composition,...
The aim of the present study was to evaluate effect that addition cholesterol-loaded cyclodextrins (CLC) thawing extender has on quality frozen-thawed boar sperm. Pooled semen (n = 5) from three boars used for experiments. cryopreserved with an egg-yolk-based extender, it diluted after in Beltsville solution (BTS) supplemented different concentrations CLC (0, 12.5, 25, 50 or 100 mg/500 × 10(6) sperm), and these samples were incubated at 37°C 150 min. following parameters sperm evaluated 30...
Cholesterol-loaded cyclodextrins (CLC) added to the sperm before cryopreservation enhance quality after freeze-thawing in several cold shock-sensitive species, including cattle and goats. However, all studies conducted date have used conventional protocols, which are cooled slowly 5°C freezing. As cholesterol plays a significant role shock resistance, it is possible that CLC-treated can withstand cooling damage when not In this study, we determined whether goat (1 mg CLC/120×106 sperm) bull...
Egg yolk (EY) and glycerol are common constituents of extenders used for sperm cryopreservation. It has been demonstrated that using cholesterol-loaded cyclodextrins (CLC) improves cryosurvival in several species. However, standard freezing might not be the most appropriate CLC-treated sperm. This study evaluated EY requirements boar spermatozoa. Semen samples from 34 ejaculates coming 4 boars were used. Each ejaculate was split into three aliquots: one untreated (control), other two treated...
The aim of this work was to examine how a pre-freezing treatment with cholesterol-loaded cyclodextrins (CLC) affects boar sperm longevity, capacitation dynamics, ability bind porcine telomerase-immortalised oviductal epithelial cell line (TERT-OPEC) in vitro and DNA integrity dynamics after freeze–thawing. Although the samples treated CLC exhibited lower quality than control (P < 0.05) immediately thawing, these differences disappeared > long-term incubation (26 h at 37 or 16°C)....