A5061629531- Adipose Tissue and Metabolism
- Animal Nutrition and Physiology
- Birth, Development, and Health
- Animal Behavior and Welfare Studies
- Neonatal Respiratory Health Research
- Antioxidant Activity and Oxidative Stress
- Infant Nutrition and Health
- Genetic and phenotypic traits in livestock
- Muscle Physiology and Disorders
- Pharmacogenetics and Drug Metabolism
- Metabolism and Genetic Disorders
- Meat and Animal Product Quality
- Neonatal and fetal brain pathology
- Effects of Environmental Stressors on Livestock
- Zebrafish Biomedical Research Applications
- Pharmacological Effects and Assays
- Diet and metabolism studies
- Agriculture Sustainability and Environmental Impact
- Pharmaceutical studies and practices
- Gestational Diabetes Research and Management
- Animal testing and alternatives
- Cardiovascular Disease and Adiposity
- Cerebral Palsy and Movement Disorders
- Reproductive Physiology in Livestock
- Congenital Diaphragmatic Hernia Studies
University of Antwerp
2017-2024
Universidad Complutense de Madrid
2012-2018
Hospital Universitari de Vic
2004
Dimethyl sulfoxide (DMSO) is a popular solvent for developmental toxicity testing of chemicals and pharmaceuticals in zebrafish embryos. In general, it recommended to keep the final DMSO concentration as low possible embryos, preferably not exceeding 100 μL/L (0.01%). However, higher concentrations are often required dissolve compounds an aqueous medium. The aim this study was determine highest that can be safely used our standardized Zebrafish Embryo Developmental Toxicity Assay (ZEDTA)....
Iberian ham production includes both purebred (IB) and Duroc-crossbred (IBxDU) pigs, which show important differences in meat quality traits, such as muscle growth fatness. This experiment was conducted to investigate gene expression differences, transcriptional regulation genetic polymorphisms that could be associated with the observed phenotypic between IB IBxDU pigs. Nine 10 pigs were slaughtered at birth. Morphometric measures blood samples obtained from Biceps femoris employed for...
The two main genetic types in Iberian pig production show important phenotypic differences growth, fattening and tissue composition since early developmental stages. objective of this work was the evaluation muscle transcriptome profile piglets both types, order to identify genes, pathways regulatory factors responsible for their differences. Contemporary families coming from pure pigs (IB) or crossing with Duroc boars (DU×IB) were generated. Piglets (14 each type) slaughtered at weaning (28...
Iberian pig production includes purebred (IB) and Duroc-crossbred (IBxDU) pigs, which show important differences in growth, fattening tissue composition. This experiment was conducted to investigate the effects of genetic type muscle (Longissimus dorsi (LD) vs Biceps femoris (BF)) on gene expression transcriptional regulation at two developmental stages. Nine IB 10 IBxDU piglets were slaughtered birth, seven four months age (growing period). Carcass traits LD intramuscular fat (IMF) content...
The effects of undernutrition during pregnancy on prenatal and postnatal development the offspring were evaluated in sows with obesity/leptin resistance. Females fed, from day 35 onwards, a diet fulfilling either 100% (group control, n=10) or 50% nutritional requirements underfed, n=10). In control group, maternal body weight increased (P<0.05) while it decreased remained steady underfed group. At days 75 100 gestation, plasma triglycerides lower but urea levels higher restricted than...
The present study aimed to determine, in a swine model of leptin resistance, the effects type and timing maternal malnutrition on growth patterns, adiposity metabolic features progeny when exposed an obesogenic diet during their juvenile development possible concomitant offspring sex. Thus, four groups were considered. A CONTROL group involved pigs born from sows fed with fulfilling daily maintenance requirements for pregnancy. treated females same but either 160% or 50% pregnancy entire...
Maternal energy restriction during pregnancy predisposes to metabolic alterations in the offspring. The present study was designed evaluate phenotypic and consequences following maternal undernutrition an obese pig model define potential role of hypothalamic gene expression programming effects. Iberian sows were fed a control or 50 % restricted diet for last two-thirds gestation. Newborns assessed body organ weights, hormonal status, genes implicated homeostasis, glucocorticoid function...
ABSTRACT Locomotion is one of the most important ecological functions in animals. Precocial animals, such as pigs, are capable independent locomotion shortly after birth. This raises question whether coordinated movement patterns and underlying muscular control these animals fully innate or there still exists a rapid maturation. We addressed this by studying gait development neonatal pigs through analysis spatio-temporal characteristics during at self-selected speed. To end, we made video...
The use of polyphenols is a promising strategy for preventing or alleviating intrauterine growth restriction (IUGR) because polyphenol supplementation increases plasma antioxidant capacity and improves oxidative stress at the feto-placental unit; which are recognized as main issues in IUGR. However, there scarcity experimental data on both realistic benefits potential hazards during gestation. Hence, we aimed to swine model IUGR pregnancy determine possible effects maternal with...
Maternal malnutrition during pregnancy, both deficiency and excess, induces changes in the intrauterine environment metabolic status of offspring, playing a key role growth, fitness/obesity appearance disorders postnatal life. There is increasing evidence that these effects may not be only limited to first generation descendants, offspring directly exposed challenges, but subsequent generations. This study evaluated, swine model obesity/leptin resistance, existence extent transgenerational...
In polytocous species, such as pigs, the growth of an individual fetus is affected by competition from its littermates and sow. This intrauterine greatly influences postnatal traits birth weight vitality (physical strength). A lowered most often observed among low piglets. Since it has been argued that locomotion might be key to unraveling vitality-related differences, we compared gait development in piglets with a (L piglets) normal (N means spatio-temporal analysis during at self-selected...
Abstract Iberian pigs and its crosses are produced to obtain high-quality meat products. The objective of this work was evaluate a wide panel DNA markers, selected by biological functional criteria, for association with traits related muscle growth, fatness, quality metabolism. We used 18 crossbred divergent postnatal growth patterns whole genome sequencing SNP discovery, over 13 million variants being detected. 1023 missense SNPs located on annotated genes showing different allele...
The Göttingen Minipig is gaining ground as nonrodent species in safety testing of drugs for pediatric indications. Due to developmental changes pharmacokinetics and pharmacodynamics, physiologically based pharmacokinetic (PBPK) models are built better predict drug exposure children aid selection nonclinical studies. These PBPK require high quality physiological ADME data such protein abundance metabolizing enzymes. available man rat, but scarce the Minipig. aim this study was assess hepatic...
Abstract Intestinal development is compromised in low birth weight (LBW) pigs, negatively impacting their growth, health, and resilience. We investigated the molecular mechanisms of altered intestinal maturation observed neonatal juvenile LBW female piglets by comparing changes morphology, gene expression, methylation versus normal (NBW) piglets. A total 16 LBW/NBW sibling pairs were sacrificed at 0 hours, 8 10 days, weeks age. The gastrointestinal tract was weighed, measured, small...
Antisense oligonucleotide (ASO) is a therapeutic modality that enables selective modulation of undruggable protein targets. However, dose- and sequence-dependent platelet count reductions have been reported in nonclinical studies clinical trials. The adult Göttingen minipig an acknowledged model for ASO safety testing, the juvenile has recently proposed testing pediatric medicines. This study assessed effects various sequences modifications on platelets using vitro activation aggregometry...
Farmers face difficulties in redeeming their investment larger litter sizes since this comes with heterogenicity, lower resilience and risk of higher mortality. Dietary oligosaccharides, given to the sow, proved beneficial for offspring's performance. However, giving oligosaccharides suckling piglet is poorly explored. Therefore, field trial studied effect dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation low (LBW, quartile), normal (NBW, two intermediate...
The zebrafish (Danio rerio) embryo is gaining interest as a bridging tool between in-vitro and in-vivo developmental toxicity studies. However, cytochrome P450 (CYP)-mediated drug metabolism in this model still under debate. Therefore, we investigated the potential of embryos larvae to bioactivate two known anti-epileptics, carbamazepine (CBZ) phenytoin (PHE), carbamazepine-10,11-epoxide (E-CBZ) 5-(4-hydroxyphenyl)-5-phenylhydantoin (HPPH), respectively. First, were exposed CBZ, PHE, E-CBZ...