Deep RNA sequencing (RNAseq) has opened a new horizon for understanding global gene expression. The functional annotation of non-model mammalian genomes including bovines is still poor compared to that human and mouse. This particularly applies tissues without direct significance milk meat production, like skin, in spite its multifunctional relevance the individual. Thus, applying an RNAseq approach, we performed whole transcriptome analysis pigmented nonpigmented bovine skin describe...

Endometrial receptivity is a prerequisite for successful embryo implantation and pregnancy. Receptivity involves complex processes promoted by many transcripts that are key components of molecular pathways depend on ovarian hormones contribute to shaping structural, metabolic, communication properties endometrial cells toward reception embryos. MicroRNAs (miRNAs) important regulators the expression these encoding effector molecules. We acquired miRNA mRNA signatures, miRNA-mRNA pairs,...

The pikeperch (Sander lucioperca) is a fresh and brackish water Percid fish natively inhabiting the northern hemisphere. This species emerging as promising candidate for intensive aquaculture production in Europe. Specific traits like cannibalism, growth rate meat quality require genomics based understanding, an optimal husbandry domestication process. Still, community lacking annotated genome sequence to facilitate genome-wide studies on pikeperch. Here, we report first highly contiguous...

Physiological processes aiding the conversion of muscle to meat involve many genes associated with structure and metabolic processes. MicroRNAs regulate networks orchestrate cellular functions, in turn regulating phenotypes.We applied weighted gene co-expression network analysis identify modules that correlated quality phenotypes were highly enriched for involved glucose metabolism, response wounding, mitochondrial ribosome, mitochondrion, extracellular matrix. Negative correlation miRNA...

Water temperature is doubtlessly one of the most important factors affecting well-being fish. For pikeperch Sander lucioperca an ambient water >20 °C has been suggested to achieve optimal growth in aquaculture farms, although natural habitats seldom reach such temperatures. We used multiplex qPCR arrays assess impact between 15 and 25 based on expression 38 potential biomarker genes characterising heat shock hypoxia response, immune activation, energy supply development liver gills...

Decades of artificial selection have markedly enhanced egg production efficiency, yet the epigenetic underpinnings, notably DNA methylation dynamics in gut, remain largely unexplored. Here, we investigate how breeds and developmental stages influence profiles laying hens, their potential relationship to performance gut health. We compared two highly selected hen strains, Lohmann Brown-Classic (LB) LSL-Classic (LSL), which exhibited similar but divergent physiological, metabolic,...

Epigenetic variation may result from selection for complex traits related to metabolic processes or appear in the course of adaptation mediate responses exogenous stressors. Moreover epigenetic marks, particular DNA methylation state, specific loci are driven by genetic variation. In this sense, polymorphism with major gene effects on and cell signaling processes, like ryanodine receptors skeletal muscle, affect methylation. DNA-Methylation profiles were generated applying Reduced...

Abstract Iberian pigs and its crosses are produced to obtain high-quality meat products. The objective of this work was evaluate a wide panel DNA markers, selected by biological functional criteria, for association with traits related muscle growth, fatness, quality metabolism. We used 18 crossbred divergent postnatal growth patterns whole genome sequencing SNP discovery, over 13 million variants being detected. 1023 missense SNPs located on annotated genes showing different allele...

MicroRNAs (miRNAs) are small non-coding RNAs that play critical roles in diverse biological processes via regulation of gene expression including skeletal muscles. In the current study, miRNA profile was investigated longissimus muscle biopsies malignant hyperthermia syndrome-negative Duroc and Pietrain pigs with distinct metabolic properties order to explore regulatory role miRNAs related mitochondrial respiratory activity enzyme muscle. A comparative analysis between performed, followed by...

Intrauterine growth restriction (IUGR) occurs in 15–20% of pig neonates and poses huge economic losses to the industry. IUGR piglets have reduced skeletal muscle growth, which may persist after birth. Prenatal is regulated by complex molecular pathways that are not well understood. MicroRNAs (miRNAs) emerged as main regulators vital biological processes body. This study was designed identify miRNA–mRNA networks regulating prenatal development pigs. We performed an integrative transcriptomic...

Kirsten rat sarcoma virus (KRAS) mutations are widespread in pancreatic ductal adenocarcinoma (PDAC) and contribute significantly to tumor initiation, progression, relapse/resistance, prognosis of patients. Although inhibitors against KRAS have been developed, this therapeutic approach is not routinely used PDAC We investigated the anti-tumor efficacy two BI-3406 (KRAS::SOS1 inhibitor) sotorasib (KRAS G12C alone or combination with MEK1/2 inhibitor trametinib and/or PI3K buparlisib seven...

Abstract Bovine neonatal pancytopenia (BNP) is a new fatal, alloimmune/alloantibody mediated disease of new-born calves induced by ingestion colostrum from cows, which had been vaccinated with specific vaccine against the Virus Diarrhoea (BVDV). The hypothesis pathogenic MHC class I molecules in put up, but no formal proof causal alleles has provided yet. However, unique features obviously result extremely high antibody titres animals, apparently also further inducing BNP. Thus,...

In the past decade number of studies investigating temperament in farm animals has increased greatly because been shown not only to affect handling but also reproduction, health and economically important production traits. However, molecular pathways underlying linking traits, reproduction have yet be studied full detail. Here we report results metabolite profiling prefrontal cortex serum cattle with distinct types that were performed further explore their divergence response slaughter...

Abstract A key common feature of all but three known mammalian genera is the strict seven cervical vertebrae blueprint, suggesting involvement strong conserving selection forces during radiation. This further supported by reports indicating that children with ribs die before they reach reproductive age. Hypotheses were put up, associating (homeotic transformations) to embryonal cancer (e.g., neuroblastoma) or ascribing constraint in vertebral count development diaphragm. Here, we describe a...

Long noncoding RNAs (lncRNAs) emerged as important regulatory component of mechanisms involved in gene expression, chromatin modification and epigenetic processes, but they are rarely annotated the bovine genome. Our study monitored jejunum transcriptome German Holstein calves fed two different milk diets using sequencing (RNA-seq). To identify potential lncRNAs within pool unknown transcripts, four bioinformatic lncRNA prediction tools were applied. The intersection alignment-free (CNCI,...

Phosphorus is an essential mineral for all living organisms and a limited resource worldwide. Variation heritability of phosphorus utilization (PU) traits were observed, indicating the general possibility improvement. Molecular mechanisms PU, including host microbial effects, are still poorly understood. The most promising molecules that interact between microbiome microRNAs. Japanese quail representing extremes PU selected from F2 population miRNA profiling ileal tissue subsequent...

Abstract Recent advances in the selective breeding of broilers and layers have made poultry production one fastest‐growing industries. In this study, a transcriptome variant calling approach from RNA‐seq data was used to determine population diversity between layers. total, 200 individuals were analyzed three different chicken populations (Lohmann Brown (LB), n = 90), Lohmann Selected Leghorn (LSL, 89), Broiler (BR, 21). The raw RNA‐sequencing reads pre‐processed, quality control checked,...

Genes expressed in mitochondria work concert with those the nucleus to mediate oxidative phosphorylation (OXPHOS), a process that is relevant for muscle metabolism and meat quality. Mitochondrial genome activity can be efficiently studied compared Duroc Pietrain pigs, which harbor different mitochondrial haplotypes distinct fiber types, respiratory activities, fat content. pigs homozygous-positive malignant hyperthermia susceptibility (PiPP) carried only haplotype 8 showed lowest absolute...

Tissue sensitivity to glucocorticoids is a key factor dictating outcome of their homeostatic and therapeutic action, whereby liver represents one the major peripheral targets. Here, we used pigs carrying natural gain-of-function glucocorticoid receptor (GR) variant Ala610Val (GRAla610Val) as model identify genes pathways related differential sensitivity. Animals with different GRAla610Val genotypes were treated either saline or two doses dexamethasone. Genome-wide transcriptional responses...

The “rat-tail” syndrome (RTS) is an inherited hypotrichosis in cattle, which exclusively expressed diluted coloured hair. affected animals also suffer from disturbed thermoregulation, impairs their health and growth performance. Phenotypic features that are similar to RTS observed dogs with black hair follicle dysplasia. We used a resource cross population between German Holstein Charolais cattle breeds prove epistatic interactions at least three independent genetic loci required for the...

The current level of knowledge on transcriptome responses triggered by endotoxins and glucocorticoids in immune cells pigs is limited. Therefore, the present study, we treated porcine peripheral blood mononuclear (PBMCs) with lipopolysaccharide (LPS) dexamethasone (DEX) separately or combined for 2 hours. resultant transcriptional were examined mRNA sequencing. We found that LPS treatment pronounced inflammatory as evidenced upregulation pro-inflammatory cytokines, chemokines, related...

Coliform mastitis is a symptom of postpartum dysgalactia syndrome (PDS), multifactorial infectious disease sows. Our previous study showed gene expression profile change after bacterial challenge porcine mammary epithelial cells (PMECs). These mRNA changes may be regulated through microRNAs (miRNAs) which play critical roles in biological processes. Therefore, miRNA was investigated PMECs. PMECs were isolated from three lactating sows and challenged with heat-inactivated potential...

Abstract Considerable variation in feed efficiency (FE) has been observed indigenous and selected meat-type chicken populations. Although this could be partially linked to extrinsic factors like diet, housing environment microbiota, it further illustrates the existence of strong molecular mechanisms enabling differential allocation resources for various physiological processes. To deepen basis individual capacity male female broilers, an RNA-seq experiment was conducted which based on a...